西藏大学学报
西藏大學學報
서장대학학보
Journal of Tibet University
2011年
2期
118~122
,共null页
芍药 花粉萌发 培养基 优化
芍藥 花粉萌髮 培養基 優化
작약 화분맹발 배양기 우화
Paeonia lactiflora; Pollen germination; Culture media; Optimization
以芍药花粉为材料，采用正交设计L^9（34）法研究蔗糖、硝酸钙、硼酸对芍药花粉离体萌发和花粉管生长的影响，筛选芍药花粉离体萌发最适宜的培养基，并研究了不同pH值对芍药花粉萌发的影响。结果表明，蔗糖对芍药花粉萌发率和花粉管生长影响最大，硼酸次之，硝酸钙影响最小；芍药花粉离体萌发最适宜培养基为，蔗糖20g·L^-1＋硼酸20mg·L^-1＋硝酸钙60mg·L^-1；其萌发率和花粉管长度分别为63．12％和183．33μm；适合芍药花粉萌发的pH为6～7。
以芍藥花粉為材料，採用正交設計L^9（34）法研究蔗糖、硝痠鈣、硼痠對芍藥花粉離體萌髮和花粉管生長的影響，篩選芍藥花粉離體萌髮最適宜的培養基，併研究瞭不同pH值對芍藥花粉萌髮的影響。結果錶明，蔗糖對芍藥花粉萌髮率和花粉管生長影響最大，硼痠次之，硝痠鈣影響最小；芍藥花粉離體萌髮最適宜培養基為，蔗糖20g·L^-1＋硼痠20mg·L^-1＋硝痠鈣60mg·L^-1；其萌髮率和花粉管長度分彆為63．12％和183．33μm；適閤芍藥花粉萌髮的pH為6～7。
이작약화분위재료，채용정교설계L^9（34）법연구자당、초산개、붕산대작약화분리체맹발화화분관생장적영향，사선작약화분리체맹발최괄의적배양기，병연구료불동pH치대작약화분맹발적영향。결과표명，자당대작약화분맹발솔화화분관생장영향최대，붕산차지，초산개영향최소；작약화분리체맹발최괄의배양기위，자당20g·L^-1＋붕산20mg·L^-1＋초산개60mg·L^-1；기맹발솔화화분관장도분별위63．12％화183．33μm；괄합작약화분맹발적pH위6～7。
The pollen of Paeonia lactiflora as materials, orthogonal experiment L9 （34） was applied to study the effects of sucrose, boric acid and calcium nitrate on in vitro pollen germination and growth in order to select the optimal culture medium for in vitro pollen germination, and the effect of different pH on pollen germination was also studied. The results showed that sucrose had significantly effect on pollen germination and growth and followed by boric acid and calcium nitrate. The optimal culture medium for in vitro pollen germination of Paeonia lactiflora was solid culture media supplemented with sugar20 g·L^-1, boric acid 20mg·L^-1and calcium nitrate 60 mg·L^-1 with pH from 6to 7, the rate of pollen germination and the pollen tube length were 63.12% and 183.33μm respectively.
