体育科学
體育科學
체육과학
China Sport Science
2011年
8期
53~58
,共null页
陈嘉勤 柳维林 张川湘 王茂材 李林
陳嘉勤 柳維林 張川湘 王茂材 李林
진가근 류유림 장천상 왕무재 리림
有氧运动 AS 炎性损伤 TLR4通路
有氧運動 AS 炎性損傷 TLR4通路
유양운동 AS 염성손상 TLR4통로
aerobic exercise; atherosclerosis; inflammation damaged; TLR4 pathway
目的：通过中小强度有氧运动和辛伐他汀药物干预,探讨AS血管炎性损伤的修复及机制。方法：应用ApoE基因敲除和高脂高胆固醇饲料诱发小鼠AS,分为动脉硬化组、运动组、用药组;ELISA测定血清炎性因子及激素水平;免疫组化法阳性标记TLR4、NF-κb、EGF、TGF-β;RT-PCR测定CRP、MCP-1 mRNA表达。结果：12周动脉硬化组ApoE基因敲除小鼠血管可见明显斑块组织;用药组内皮表层细胞肿胀呈泡沫样,运动组则未见以上组织改变;运动和用药组小鼠血清IL-6、TNF-a含量和血管组织TLR4、NF-κb、CRP、MCP-1表达均降低（P〈0.05）,而血清IL-10、ET1、AngⅡ和内皮细胞EGF、TGF-βmRNA表达均升高（P〈0.05）。结论：有氧运动有效防治AS小鼠血管炎性损伤,其可能机制是介导了Toll样受体通路并恢复血管激素分泌与促进内皮细胞再生。
目的：通過中小彊度有氧運動和辛伐他汀藥物榦預,探討AS血管炎性損傷的脩複及機製。方法：應用ApoE基因敲除和高脂高膽固醇飼料誘髮小鼠AS,分為動脈硬化組、運動組、用藥組;ELISA測定血清炎性因子及激素水平;免疫組化法暘性標記TLR4、NF-κb、EGF、TGF-β;RT-PCR測定CRP、MCP-1 mRNA錶達。結果：12週動脈硬化組ApoE基因敲除小鼠血管可見明顯斑塊組織;用藥組內皮錶層細胞腫脹呈泡沫樣,運動組則未見以上組織改變;運動和用藥組小鼠血清IL-6、TNF-a含量和血管組織TLR4、NF-κb、CRP、MCP-1錶達均降低（P〈0.05）,而血清IL-10、ET1、AngⅡ和內皮細胞EGF、TGF-βmRNA錶達均升高（P〈0.05）。結論：有氧運動有效防治AS小鼠血管炎性損傷,其可能機製是介導瞭Toll樣受體通路併恢複血管激素分泌與促進內皮細胞再生。
목적：통과중소강도유양운동화신벌타정약물간예,탐토AS혈관염성손상적수복급궤제。방법：응용ApoE기인고제화고지고담고순사료유발소서AS,분위동맥경화조、운동조、용약조;ELISA측정혈청염성인자급격소수평;면역조화법양성표기TLR4、NF-κb、EGF、TGF-β;RT-PCR측정CRP、MCP-1 mRNA표체。결과：12주동맥경화조ApoE기인고제소서혈관가견명현반괴조직;용약조내피표층세포종창정포말양,운동조칙미견이상조직개변;운동화용약조소서혈청IL-6、TNF-a함량화혈관조직TLR4、NF-κb、CRP、MCP-1표체균강저（P〈0.05）,이혈청IL-10、ET1、AngⅡ화내피세포EGF、TGF-βmRNA표체균승고（P〈0.05）。결론：유양운동유효방치AS소서혈관염성손상,기가능궤제시개도료Toll양수체통로병회복혈관격소분비여촉진내피세포재생。
Objective： Indicated of the effects and mechanisms of atherosclerosis vascular inflammation damaged by medium-intensity aerobic exercise and simvastatin pharmaceutical on ApoE knock out mice.Methods： Induced atherosclerosis model through ApoE gene knockout and high fat and high cholesterol fed in mice,and were divided into atherosclerosis groups,exercise groups,drug groups;ELISA assay viewed of serum inflammations and hormone levels;TLR4,NF-κb,EGF,TGF-βlevels detected by immunohistochemistry assay;RT-PCR assay detection of CRP,MCP-1mRNA expression.Results： The atherosclerosis groups showed obvious plaque tissue endothelial cells on ApoE knock out mice for 12 weeks;the drug groups showed endothelial cell swelling and foamy surface,the exercise groups were no more than organizational changed.The serum of IL-6,TNF-a levels and TLR4,NF-κb,CRP,MCP-1expression in vascular of the exercise and drug groups were lower than the quite groups（P0.05）,while IL-10,ET1,AngⅡ levels and EGF,TGF-β mRNA expression increased（P0.05）.Conclusion： It＇s effectively improved vascular inflammation damaged by aerobic exercise,and the possible mechanism mediated Toll-like receptor signaling pathway,while restored hormone secretion and promoted vascular endothelial cell regeneration.
