山东体育学院学报
山東體育學院學報
산동체육학원학보
Journal of Shandong Institute Of Physical Education And Sports
2011年
7期
53~57
,共null页
扇贝肽 运动员停训综合症 骨骼肌萎缩 Caspase-3活性 抗氧化能力 大鼠
扇貝肽 運動員停訓綜閤癥 骨骼肌萎縮 Caspase-3活性 抗氧化能力 大鼠
선패태 운동원정훈종합증 골격기위축 Caspase-3활성 항양화능력 대서
scallop peptides ; athletic detraining syndrome ; muscular atrophy ; activity of caspase - 3 ; antioxidant capacity ; rat
目的：通过测试萎缩骨骼肌细胞Caspase-3、SOD活性和MDA含量，观察补充扇贝肽对运动大鼠停训导致骨骼肌萎缩后肌细胞Caspase-3活性及其抗氧化能力的影响，研究扇贝肽对停训后骨骼肌萎缩的干预作用。方法：40只SD雄性大鼠以15m／min的速度，持续性水平跑台训练30min开始进行递增负荷运动，运动6d／周，休息1d／周，速度递增3m／min／周，时间递增30min／周，运动3周后即刻处死10只大鼠为基础对照组，其余大鼠随机分组为补充安慰剂阴性对照组、补充牛乳阳性对照组和补充扇贝肽实验组，30只大鼠左侧后肢实施石膏固定模拟停训模型，并进行扇贝肽补充实验。实验组和对照组所有动物每天分别进行灌胃15％扇贝肽饮料2mL、等量的安慰剂和牛乳。结果：发现补充扇贝肽可显著减低停训后萎缩骨骼肌细胞MDA含量增加，增加SOD活性，提高细胞内抗氧化能力，可显著抑制停训后萎缩骨骼肌细胞Caspase-3的活性，从而抑制骨骼肌收缩蛋白和结构蛋白的降解，促进萎缩骨骼肌的恢复。
目的：通過測試萎縮骨骼肌細胞Caspase-3、SOD活性和MDA含量，觀察補充扇貝肽對運動大鼠停訓導緻骨骼肌萎縮後肌細胞Caspase-3活性及其抗氧化能力的影響，研究扇貝肽對停訓後骨骼肌萎縮的榦預作用。方法：40隻SD雄性大鼠以15m／min的速度，持續性水平跑檯訓練30min開始進行遞增負荷運動，運動6d／週，休息1d／週，速度遞增3m／min／週，時間遞增30min／週，運動3週後即刻處死10隻大鼠為基礎對照組，其餘大鼠隨機分組為補充安慰劑陰性對照組、補充牛乳暘性對照組和補充扇貝肽實驗組，30隻大鼠左側後肢實施石膏固定模擬停訓模型，併進行扇貝肽補充實驗。實驗組和對照組所有動物每天分彆進行灌胃15％扇貝肽飲料2mL、等量的安慰劑和牛乳。結果：髮現補充扇貝肽可顯著減低停訓後萎縮骨骼肌細胞MDA含量增加，增加SOD活性，提高細胞內抗氧化能力，可顯著抑製停訓後萎縮骨骼肌細胞Caspase-3的活性，從而抑製骨骼肌收縮蛋白和結構蛋白的降解，促進萎縮骨骼肌的恢複。
목적：통과측시위축골격기세포Caspase-3、SOD활성화MDA함량，관찰보충선패태대운동대서정훈도치골격기위축후기세포Caspase-3활성급기항양화능력적영향，연구선패태대정훈후골격기위축적간예작용。방법：40지SD웅성대서이15m／min적속도，지속성수평포태훈련30min개시진행체증부하운동，운동6d／주，휴식1d／주，속도체증3m／min／주，시간체증30min／주，운동3주후즉각처사10지대서위기출대조조，기여대서수궤분조위보충안위제음성대조조、보충우유양성대조조화보충선패태실험조，30지대서좌측후지실시석고고정모의정훈모형，병진행선패태보충실험。실험조화대조조소유동물매천분별진행관위15％선패태음료2mL、등량적안위제화우유。결과：발현보충선패태가현저감저정훈후위축골격기세포MDA함량증가，증가SOD활성，제고세포내항양화능력，가현저억제정훈후위축골격기세포Caspase-3적활성，종이억제골격기수축단백화결구단백적강해，촉진위축골격기적회복。
Objective： The effects of scallop peptides supplementation on the activity of caspase -3 and SOD and the content of MDA in skeletal muscle after detraining were investigated. To study the effects of scallop peptides supplementation on muscular atrophy after detraining in rats. Methods ： 40 male Sprague -Dawley rats ran 30 minutes at speed of 15 m/min , exercise 5 day/week and rest 1 day/week in the first week. In the second week, rats ran 60 minutes at speed of 18 m/min, exercise 5 day/week and rest 1 day/week and ran 90 minutes at speed of 21 m/min, exercise 5 day/week and rest 1 day/week in the third week. After sports training for 3 weeks, Ten rats were killed as baseline group. The 30 rats were randomly divided into 3 groups as placebo, milk and SPs supplemented, Then the left hind limbs of the rats were fixed by plastic gypsum for 3 weeks. Rats in experimental groups were fed 2ml beverage containing 15% SPs, and rats in control were fed 2ml placebo and milk respectively. Results： The results showed that After SPs supplementation, the activity of caspase -3 decreased obviously, and the antioxidant capacity increased obviously in atrophic muscle in raes.
