CAJ | 학술논문

目的：探讨SD雄性大鼠在一次性力竭运动后，大脑皮质微结构和超微结构的形态改变与神经生长因子NGF、受体TrkA的动态变化关系。方法：随机将大鼠分为对照组（C组）、一次性力竭游泳运动后即刻组（E1）、12h组（E2）、24h组（E3）、48h组（E4），灌注定位取出大脑皮质，运用HE染色、铀铅双染，在光、电镜下观察大脑皮质结构变化，运用免疫组化技术检测大脑皮质中NGF和TrkA表达变化。结果：NGF、TrkA表达水平较C组显著性上升（P〈0．05）；E2组的NGF、TrkA的表达水平较E1组下调，其中TrkA的表达下调显著（P〈0．05）；NGF、TrkA的表达上升最为显著，较C组（P〈0．01）；E4组继续维持着高表达，较C组（P〈0．01）。结论：一次性力竭运动后大脑皮质结构的损伤和重塑与NGF、TrkA动态表达具有一定的联系性。
목적：탐토SD웅성대서재일차성력갈운동후，대뇌피질미결구화초미결구적형태개변여신경생장인자NGF、수체TrkA적동태변화관계。방법：수궤장대서분위대조조（C조）、일차성력갈유영운동후즉각조（E1）、12h조（E2）、24h조（E3）、48h조（E4），관주정위취출대뇌피질，운용HE염색、유연쌍염，재광、전경하관찰대뇌피질결구변화，운용면역조화기술검측대뇌피질중NGF화TrkA표체변화。결과：NGF、TrkA표체수평교C조현저성상승（P〈0．05）；E2조적NGF、TrkA적표체수평교E1조하조，기중TrkA적표체하조현저（P〈0．05）；NGF、TrkA적표체상승최위현저，교C조（P〈0．01）；E4조계속유지착고표체，교C조（P〈0．01）。결론：일차성력갈운동후대뇌피질결구적손상화중소여NGF、TrkA동태표체구유일정적련계성。
Objective： To investigate the dynamic relationship between the morphological changes of the micro - structure and ultrastructural of the cerebral cortex and nerve growth factor（NGF） and TrkA receptor in the SD male rats after one - time exhaustive exercise. Methods ： After a model of one - time exhaustive swimming, rats are randomly di- vided into control group （C group） immediately after exercise group （El） , 12h group （E2） , 24h group （E3） , 48h group （E4）. The cerebral cortex is removed after perfusion and position. By using HE staining, uranium - lead double staining, the ultrastructural and micro - structure change of cerebral cortex are observed in light microscopy and electron microscopy. The expression of NGF and TrkA in cerebral cortex is detected depending on immunohistochemistry and im- age analysis. Result ： Immediately after one time exhaustive exercise, the brain structure shows early cortical ischemia - like changes; NGF, TrkA expression levels are more tex shows apparent structural damage after 12h, but significantly increased than that in the control group; Cerebral cor- the NGF, TrkA expression levels down; compared with E1 the ex- pression of TrkA is significant decreased ; structural damage in cerebral cortex progressively increases after 24h, NGF, TrkA expression are most significantly increased after 24h; the cerebral cortex structure begins to reshape after 48h; NGF, TrkA expression levels have come down but continue to maintain a high expression. Conclusion： There is a certain dynamic association between structural damage and remodeling of cerebral cortex with NGF, TrkA expression after one - time exhaustive exercise.