CAJ | 학술논문

目的：探讨不同运动强度的耐力运动对糖尿病大鼠肾脏氧化应激及基质金属蛋白酶组织抑制物-1（TIMP-1）的影响。方法：以体重190±15g SD雄性大鼠为研究对象，经6周高脂高糖膳食喂养并腹腔注射链脲佐菌素建立糖尿病模型后，将糖尿病大鼠分为糖尿病对照组（DMC），糖尿病运动I纽（DME1，跑逮为10m／rain），糖尿病运动Ⅱ组（DME2，跑速为15m／min），糖尿病运动3组（DME3，跑速为20m／min），运动组每天1小时跑台运动，每周5天，持续6周。6周末检测血糖、胰岛素，肾脏组织内MDA、SOD、CAT及TIMP-1的含量或活性。结果：与对照组相比，运动组血糖均著下降（P〈0．01），而运动组血清胰岛素均呈上升趋势，且DME3组显著上升（P〈0．05），但运动1组、运动2组胰岛素抵抗指数显著下降（P〈0．05）；而运动1组与运动2组MDA显著下降（P〈0．05，P〈0．01）；同时，各运动组抗氧化酶均呈上升趋势，其中T—SOD含量上升均具有显著性或极显著性差异，低强度、中强度运动组CAT含量显著高于对照组（P〈0．01）；而运动组TIMP—1表达水平显著或极显著下降（P〈0．05，P〈0．01）。结论：10m／min、15m／min强度耐力运动可有效地控制降低血糖，改善胰岛素抵抗，提高糖尿病大鼠抗氧化防御系统，防止肾脏纤维化，有利于控制糖尿病进一步恶化。
목적：탐토불동운동강도적내력운동대당뇨병대서신장양화응격급기질금속단백매조직억제물-1（TIMP-1）적영향。방법：이체중190±15g SD웅성대서위연구대상，경6주고지고당선식위양병복강주사련뇨좌균소건립당뇨병모형후，장당뇨병대서분위당뇨병대조조（DMC），당뇨병운동I뉴（DME1，포체위10m／rain），당뇨병운동Ⅱ조（DME2，포속위15m／min），당뇨병운동3조（DME3，포속위20m／min），운동조매천1소시포태운동，매주5천，지속6주。6주말검측혈당、이도소，신장조직내MDA、SOD、CAT급TIMP-1적함량혹활성。결과：여대조조상비，운동조혈당균저하강（P〈0．01），이운동조혈청이도소균정상승추세，차DME3조현저상승（P〈0．05），단운동1조、운동2조이도소저항지수현저하강（P〈0．05）；이운동1조여운동2조MDA현저하강（P〈0．05，P〈0．01）；동시，각운동조항양화매균정상승추세，기중T—SOD함량상승균구유현저성혹겁현저성차이，저강도、중강도운동조CAT함량현저고우대조조（P〈0．01）；이운동조TIMP—1표체수평현저혹겁현저하강（P〈0．05，P〈0．01）。결론：10m／min、15m／min강도내력운동가유효지공제강저혈당，개선이도소저항，제고당뇨병대서항양화방어계통，방지신장섬유화，유리우공제당뇨병진일보악화。
Objective：To investigate the effects of exercise with different intensities on oxidative stress of the kidneys of diabetic rats. Methods：The diabetic model was induced by 6 weeks high -fat high -sugar diet and injection of streptozotocin （STZ）. The rats were divided into 4 groups： diabetes control group（DMC） ,diabetes exercise intervention group 1 （DME1, the speed 10m/min） , diabetes exercise intervention group 2 （DME2, the speed 15 m/min）and diabetes exercise intervention group 3 （DME3 ,the speed 20m/min）. The mice of exercise intervention groups were exercised for an hour everyday at requirement intensity 5days/week for 6 weeks,following which serum fasting blood glu- cose （FBG） , fasting insulin （ FINS）, renal oxidative stress and TIMP - 1 were evaluated. Results ：Compared with DMC group ： the FBG levels decreased significantly in exercise groups （ P 〈 0.01 ）. FINS of exercise groups increased, and showed significant differences in DME3 group （P 〈 0.05 ）. IRI in DME1 and DME2 have significantly decreased（P 〈0.05）. The renal MDA of DME1, DME2 gourps were significantly and very significantly reduced（ P 〈 0. 05 ,P 〈 0. 01 ）. While the level of an- tioxidant enzymes increased in the three exercise groups;significant differences of CAT in DME1 and DME2 were found（P 〈0.01 ） ; TIMP - 1 expression level of exercise groups had significant or very significant decline （ P 〈 0.05, P 〈 0. 01 ）. Conclusion ： Low and moderate intensity endurance exercise could effectively reduce FBG, improve insulin resistance,increase the level of renal antioxidant defense system and prevent renal fibrosis.