CAJ | 학술논문

目的：研究FGFR2IIIc及其突变型腺病毒对小鼠乳腺癌的作用。方法：确定腺病毒颗粒Ad、Ad-FGFR2IIIc及Ad-FGFR2IIIcS252W感染4T1细胞的最佳MOI,并通过RT-PCR检测外源目的基因的表达。建立BALB/c小鼠的4T1乳腺癌模型,在此模型上原位注射重组腺病毒颗粒治疗17天,观察其对小鼠肿瘤的作用。结果：当MOI=60时腺病毒对4T1细胞的感染率可达到95%以上,RT-PCR结果显示,FGFR2IIIc和FGFR2IIIcS252W可在4T1细胞中成功表达。与对照组和Ad组比较,Ad-FGFR2IIIc对小鼠肿瘤无明显作用,Ad-FGFR2IIIcS252W能显著减缓小鼠肿瘤体积和质量的增长。结论：FGFR2IIIc对BALB/c小鼠4T1乳腺癌无显著作用,FGFR2IIIcS252W能够显著抑制BALB/c小鼠4T1乳腺癌的生长。
목적：연구FGFR2IIIc급기돌변형선병독대소서유선암적작용。방법：학정선병독과립Ad、Ad-FGFR2IIIc급Ad-FGFR2IIIcS252W감염4T1세포적최가MOI,병통과RT-PCR검측외원목적기인적표체。건립BALB/c소서적4T1유선암모형,재차모형상원위주사중조선병독과립치료17천,관찰기대소서종류적작용。결과：당MOI=60시선병독대4T1세포적감염솔가체도95%이상,RT-PCR결과현시,FGFR2IIIc화FGFR2IIIcS252W가재4T1세포중성공표체。여대조조화Ad조비교,Ad-FGFR2IIIc대소서종류무명현작용,Ad-FGFR2IIIcS252W능현저감완소서종류체적화질량적증장。결론：FGFR2IIIc대BALB/c소서4T1유선암무현저작용,FGFR2IIIcS252W능구현저억제BALB/c소서4T1유선암적생장。
A study on the impact of FGFR2IIIc and its mutated S252W Adenovirus on mice was conducted by RT-PCR.4T1detecting expression of exogenous target genes of infected 4T1 cells with Ad,Ad-FGFR2IIIc and Ad-FGFR2IIIcS252W respectively.A breast cancer model was established in BALB/c mice,and then recombinant adenoviruses were injected in situ to investigate their impacts on the mice＇s tumor.The results showed that the infection rate of adenovirus on 4T1 cells could reach above 95% as MOI=60 and RT-PCR showed that FGFR2IIIc and FGFR2IIIcS252W could be expressed successfully in 4T1 cells.Ad-FGFR2IIIc had no apparent effect on the mice＇s tumor compare to Ad-FGFR2IIIcS252W which could inhibit growth of mice tumor and growth of 4T1 breast cancer in BALB/c mice obviously.