体育科学
體育科學
체육과학
China Sport Science
2011年
12期
55~61
,共null页
马治云 潘珊珊 申毓军 郝喆 葛珺 王凯
馬治雲 潘珊珊 申毓軍 郝喆 葛珺 王凱
마치운 반산산 신육군 학철 갈군 왕개
氨基末端-脑钠肽前体 运动预适应 心肌保护效应 力竭运动 鼠 动物实验
氨基末耑-腦鈉肽前體 運動預適應 心肌保護效應 力竭運動 鼠 動物實驗
안기말단-뇌납태전체 운동예괄응 심기보호효응 력갈운동 서 동물실험
NT- proBNP ; exercise preconditioning cardioprotective effect exhaustive exer-cise rat
目的：以运动预适应（EP）诱导心肌保护效应为研究基础，以心脏分子标志物NT—proBNP为研究核心，联合应用血清NT—proBNP与血清clhI和心肌HBFP染色，综合评价EP对力竭运动心肌的保护效应；方法：SD大鼠100只，随机分为对照组（C组）、力竭组（EE组）、运动预适应组（EP组）和运动预适应＋力竭组（EP＋EE组）。采用一次间歇性大强度跑台运动建立EP模型，力竭跑台运动建立急性心肌损伤模型。用ELIsA法检测血清NT—proBNP浓度，CLIA法检测血清cTnI浓度，HBFP染色法观察心肌缺血缺氧状况；结果：与C组比较，EE组血清NT—proBNP浓度显著升高（P〈0．05），EP组变化不明显（P〉0．05）；与EP组比较，EP＋EE纽血清NT-proBNP浓度变化不明显（P〉0．05）；与EE组比较，EP＋EE组血清NT—proBNP浓度显著下降（P〈0．05）。各组大鼠血清NT—proBNP浓度与血清cTnI浓度、心肌HBFP染色结果的变化趋势一致；结论：EP是一种非损伤性预适应方式，能明显减轻力竭运动引起的急性心肌损伤和促进心脏功能恢复。血清NT—proBNP与血清cTnI试和心肌聊染色联合应用具有互补性，有助于综合评价EP对力竭运动心肌的保护效应。
目的：以運動預適應（EP）誘導心肌保護效應為研究基礎，以心髒分子標誌物NT—proBNP為研究覈心，聯閤應用血清NT—proBNP與血清clhI和心肌HBFP染色，綜閤評價EP對力竭運動心肌的保護效應；方法：SD大鼠100隻，隨機分為對照組（C組）、力竭組（EE組）、運動預適應組（EP組）和運動預適應＋力竭組（EP＋EE組）。採用一次間歇性大彊度跑檯運動建立EP模型，力竭跑檯運動建立急性心肌損傷模型。用ELIsA法檢測血清NT—proBNP濃度，CLIA法檢測血清cTnI濃度，HBFP染色法觀察心肌缺血缺氧狀況；結果：與C組比較，EE組血清NT—proBNP濃度顯著升高（P〈0．05），EP組變化不明顯（P〉0．05）；與EP組比較，EP＋EE紐血清NT-proBNP濃度變化不明顯（P〉0．05）；與EE組比較，EP＋EE組血清NT—proBNP濃度顯著下降（P〈0．05）。各組大鼠血清NT—proBNP濃度與血清cTnI濃度、心肌HBFP染色結果的變化趨勢一緻；結論：EP是一種非損傷性預適應方式，能明顯減輕力竭運動引起的急性心肌損傷和促進心髒功能恢複。血清NT—proBNP與血清cTnI試和心肌聊染色聯閤應用具有互補性，有助于綜閤評價EP對力竭運動心肌的保護效應。
목적：이운동예괄응（EP）유도심기보호효응위연구기출，이심장분자표지물NT—proBNP위연구핵심，연합응용혈청NT—proBNP여혈청clhI화심기HBFP염색，종합평개EP대력갈운동심기적보호효응；방법：SD대서100지，수궤분위대조조（C조）、력갈조（EE조）、운동예괄응조（EP조）화운동예괄응＋력갈조（EP＋EE조）。채용일차간헐성대강도포태운동건립EP모형，력갈포태운동건립급성심기손상모형。용ELIsA법검측혈청NT—proBNP농도，CLIA법검측혈청cTnI농도，HBFP염색법관찰심기결혈결양상황；결과：여C조비교，EE조혈청NT—proBNP농도현저승고（P〈0．05），EP조변화불명현（P〉0．05）；여EP조비교，EP＋EE뉴혈청NT-proBNP농도변화불명현（P〉0．05）；여EE조비교，EP＋EE조혈청NT—proBNP농도현저하강（P〈0．05）。각조대서혈청NT—proBNP농도여혈청cTnI농도、심기HBFP염색결과적변화추세일치；결론：EP시일충비손상성예괄응방식，능명현감경력갈운동인기적급성심기손상화촉진심장공능회복。혈청NT—proBNP여혈청cTnI시화심기료염색연합응용구유호보성，유조우종합평개EP대력갈운동심기적보호효응。
Objective： On the basis of the cardioprotective effect of exercise preconditioning （EP）, this study was designed to focus cardiac molecular marker NT-proBNP to comprehensively assess the protective effect of EP on the heart undergoing the exhaustive exercise by means of the associated application of NT-proBNP , cTnI and HBFP staining. Methods： One hundred SD rats were randomly assigned to control group, EE group, EP group and EP＋ EE group. The rat model of EP was set up by means of the single bout of intermittent treadmill exercise characterizing the high intensity, and the rat model of acute myocardial injury in the manner of exhaustive treadmill exercise. The serum concentration of NT-proBNP and cTnI and myocardium staining were measured by ELISA, CLIA or HBFP array, respectively. Results ： Compared with Group C, Group EE significantly increased in the serum concentration of NT- proBNP（P〈0.05）, and Group EP appeared to decrease slightly （P〉0.05）;Compared with Group EP,Group EP＋EE showed a little enhancement（P〉0. 05）; Compared with Group EE, Group EP ＋ EE markedly decreased （P〈0.05）. The changing tendency of Serum concen- tration of NT-proBNP and cTnI and myocardium HBFP staining on rats from the different groups was comparably consistent. Conclusions： EP is one of non-injury method of preconditioning, which can significantly attenuate the acute myocardial injury caused by the exhaustive exercise and promote the recovery of the heart function. The associated utilization of Serum concentration of NT-proBNP and cTnI and myocardium staining is well complementary, and is contributed to comprehensively assess the protective effect of EP on the heart undergoing the exhaustive exercise.
