北京体育大学学报
北京體育大學學報
북경체육대학학보
Journal of Beijing University of Physical Education
2011年
11期
47~50
,共null页
石丽君 李珊珊 熊开宇 龚丽景
石麗君 李珊珊 熊開宇 龔麗景
석려군 리산산 웅개우 공려경
有氧运动 功能重塑 胸主动脉 平滑肌
有氧運動 功能重塑 胸主動脈 平滑肌
유양운동 공능중소 흉주동맥 평활기
aerobic exercise; functional remodeling; thoracic aorta; smooth muscle
目的：探讨有氧运动对大鼠心血管和胸主动脉平滑肌的功能重塑作用及可能的钾通道机制。方法：选用2月龄雄性Wistar大鼠20只,随机分为安静对照组和有氧运动组,进行8周跑台运动。8周后,选取部分动物（各组5只）进行股动静脉插管,插管留置体内并从颈部皮肤切口穿出固定。术后恢复1 d后,于在体、清醒状态下测定基础血压、心率,并经股静脉注射Ang II和非特异性钾通道阻断剂TEA,观察其心血管反应。另10只大鼠,开胸取胸主动脉,去内皮,并制备血管环,进行离体血管收缩特性检测。结果：1）有氧运动后大鼠的安静心率显著下降;平均动脉压有下降趋势,但与安静对照组比无显著性差异;2）静脉注射Ang II引起的升压反应幅度减小;3）静脉注射非特异性钾通道阻断剂TEA可诱发升压反应,且运动组较安静对照组显著;4）两组去内皮胸主动脉血管环对于Ang II（10-9~10-4M）均有浓度依赖性收缩,但有氧运动组的最大张力显著低于安静对照组;5）TEA（5×10-3M）可诱发大鼠血管张力增加,运动组升高幅度显著大于安静组。结论：有氧运动可诱导大鼠心血管和胸主动脉平滑肌出现功能重塑,其中钾通道活性的增强可能是重要机制之一。
目的：探討有氧運動對大鼠心血管和胸主動脈平滑肌的功能重塑作用及可能的鉀通道機製。方法：選用2月齡雄性Wistar大鼠20隻,隨機分為安靜對照組和有氧運動組,進行8週跑檯運動。8週後,選取部分動物（各組5隻）進行股動靜脈插管,插管留置體內併從頸部皮膚切口穿齣固定。術後恢複1 d後,于在體、清醒狀態下測定基礎血壓、心率,併經股靜脈註射Ang II和非特異性鉀通道阻斷劑TEA,觀察其心血管反應。另10隻大鼠,開胸取胸主動脈,去內皮,併製備血管環,進行離體血管收縮特性檢測。結果：1）有氧運動後大鼠的安靜心率顯著下降;平均動脈壓有下降趨勢,但與安靜對照組比無顯著性差異;2）靜脈註射Ang II引起的升壓反應幅度減小;3）靜脈註射非特異性鉀通道阻斷劑TEA可誘髮升壓反應,且運動組較安靜對照組顯著;4）兩組去內皮胸主動脈血管環對于Ang II（10-9~10-4M）均有濃度依賴性收縮,但有氧運動組的最大張力顯著低于安靜對照組;5）TEA（5×10-3M）可誘髮大鼠血管張力增加,運動組升高幅度顯著大于安靜組。結論：有氧運動可誘導大鼠心血管和胸主動脈平滑肌齣現功能重塑,其中鉀通道活性的增彊可能是重要機製之一。
목적：탐토유양운동대대서심혈관화흉주동맥평활기적공능중소작용급가능적갑통도궤제。방법：선용2월령웅성Wistar대서20지,수궤분위안정대조조화유양운동조,진행8주포태운동。8주후,선취부분동물（각조5지）진행고동정맥삽관,삽관류치체내병종경부피부절구천출고정。술후회복1 d후,우재체、청성상태하측정기출혈압、심솔,병경고정맥주사Ang II화비특이성갑통도조단제TEA,관찰기심혈관반응。령10지대서,개흉취흉주동맥,거내피,병제비혈관배,진행리체혈관수축특성검측。결과：1）유양운동후대서적안정심솔현저하강;평균동맥압유하강추세,단여안정대조조비무현저성차이;2）정맥주사Ang II인기적승압반응폭도감소;3）정맥주사비특이성갑통도조단제TEA가유발승압반응,차운동조교안정대조조현저;4）량조거내피흉주동맥혈관배대우Ang II（10-9~10-4M）균유농도의뢰성수축,단유양운동조적최대장력현저저우안정대조조;5）TEA（5×10-3M）가유발대서혈관장력증가,운동조승고폭도현저대우안정조。결론：유양운동가유도대서심혈관화흉주동맥평활기출현공능중소,기중갑통도활성적증강가능시중요궤제지일。
Objective： To investigate the aerobic exercise-induced functional remodeling of the rat cardiovascular systmem and thoracic aortic smooth muscle,and to explore the possible potassium channel mechanisms underlying these effects.Methods： 20 male Wistar rats（2M） were randomly divided into sedentary group and exercise group.Training protocol： 8 week treadmill.After 8 weeks,some animals（n=5 in each group） underwent femoral arterial and venous cannulation.Catheters were tunneled subcutaneously,exteriorized at the nape of the neck.Following one day recovery,the basal blood pressure,heart rate and the cardiovascular responses to intravenous（i.v.） injection of Ang II and non-specific K＋ channel blocker TEA were monitored in vivo.In the other 10 animals,the thoracic aorta was carefully exposed after thoracotomy and dissected free.Segments were cut into rings and the endothelial cell lining was removed.The vessel contractility was examined in vitro.Results：1） After the aerobic exercises,the heart rate at rest reduced significantly.The mean arterial pressure of rats had a tendency to decrease but there was no significant difference between the sedentary and the exercise groups at rest.2）The pressor responses induced by i.v.injection of Ang II were attenuated in the exercise group compared with that in the sedentary group;3） The pressor responses induced by i.v.injection of TEA,was significantly enhanced after exercise training;4）Ang II（10-9～10-4M） caused a significantly increase of vascular tone in a concentration-dependent manner in both groups,while the increase amplitude was less in exercise group than in the sedentary group;5） TEA（5×10-3 M） could induce an increase of vessel contractility in two groups,while the amplitude of increase was significantly enhanced after exercise training.Conclusion： Aerobic exercise training can induce functional remodeling of the rat cardiovascular system and thoracic aorta smooth muscle,in which the enhancement of the potassium channel activity is a very possible mechanism.
