北京体育大学学报
北京體育大學學報
북경체육대학학보
Journal of Beijing University of Physical Education
2012年
2期
54~58
,共null页
慢性束缚 慢性疲劳综合征大鼠（CFS大鼠） 运动干预
慢性束縳 慢性疲勞綜閤徵大鼠（CFS大鼠） 運動榦預
만성속박 만성피로종합정대서（CFS대서） 운동간예
chronic restraint; chronic fatigue syndrome rats; motion invention
目的：通过研究游泳运动对大鼠慢性疲劳综合征形成的干预作用及对γ-干扰素、白介素4等指标的影响,为防治CFS提供分子学方面的依据。方法：将50只8周龄Wister雄性大鼠,随机分为实验组和对照组。实验结束24 h后处死。取2-3 mL的血液低温离心分离血清于-20℃冰箱中储存备用待测皮质醇和血睾酮;取3-4 mL的血液低温离心分离血清,置于4℃冰箱中保存待测INF-γ和IL-4。结果：1）采用慢性束缚方法复制的动物模型血清睾酮值、胸腺重量非常显著性降低,皮质酮值非常显著性升高。2）不同时间的游泳对慢性束缚大鼠产生的效果不同,与模型对照组相比：（1）30 min游泳组和60 min游泳组的体重、力竭时间依次为非常显著性升高和显著性升高,血清睾酮及胸腺重量显著性升高,皮质酮值显著降低;（2）慢性束缚＋力竭游泳组体重、力竭时间、血清睾酮和胸腺重量有降低趋势,皮质酮有升高趋势。3）慢性束缚可使大鼠的IFN-γ的含量非常显著性降低;4）慢性束缚可使大鼠的IL-4的含量降低,但效果不显著;各组之间无显著性差异。结论：1）慢性束缚可以成功复制慢性疲劳综合征大鼠模型。2）30min、60 min游泳特别是30 m游泳的干预作用可有效预防CFS的发生。3）30 m、60 m游泳都可以调节大鼠FIN-γ的分泌,使IFN-γ/IL-4、比率正向变化,有效预防大鼠CFS的形成。
目的：通過研究遊泳運動對大鼠慢性疲勞綜閤徵形成的榦預作用及對γ-榦擾素、白介素4等指標的影響,為防治CFS提供分子學方麵的依據。方法：將50隻8週齡Wister雄性大鼠,隨機分為實驗組和對照組。實驗結束24 h後處死。取2-3 mL的血液低溫離心分離血清于-20℃冰箱中儲存備用待測皮質醇和血睪酮;取3-4 mL的血液低溫離心分離血清,置于4℃冰箱中保存待測INF-γ和IL-4。結果：1）採用慢性束縳方法複製的動物模型血清睪酮值、胸腺重量非常顯著性降低,皮質酮值非常顯著性升高。2）不同時間的遊泳對慢性束縳大鼠產生的效果不同,與模型對照組相比：（1）30 min遊泳組和60 min遊泳組的體重、力竭時間依次為非常顯著性升高和顯著性升高,血清睪酮及胸腺重量顯著性升高,皮質酮值顯著降低;（2）慢性束縳＋力竭遊泳組體重、力竭時間、血清睪酮和胸腺重量有降低趨勢,皮質酮有升高趨勢。3）慢性束縳可使大鼠的IFN-γ的含量非常顯著性降低;4）慢性束縳可使大鼠的IL-4的含量降低,但效果不顯著;各組之間無顯著性差異。結論：1）慢性束縳可以成功複製慢性疲勞綜閤徵大鼠模型。2）30min、60 min遊泳特彆是30 m遊泳的榦預作用可有效預防CFS的髮生。3）30 m、60 m遊泳都可以調節大鼠FIN-γ的分泌,使IFN-γ/IL-4、比率正嚮變化,有效預防大鼠CFS的形成。
목적：통과연구유영운동대대서만성피로종합정형성적간예작용급대γ-간우소、백개소4등지표적영향,위방치CFS제공분자학방면적의거。방법：장50지8주령Wister웅성대서,수궤분위실험조화대조조。실험결속24 h후처사。취2-3 mL적혈액저온리심분리혈청우-20℃빙상중저존비용대측피질순화혈고동;취3-4 mL적혈액저온리심분리혈청,치우4℃빙상중보존대측INF-γ화IL-4。결과：1）채용만성속박방법복제적동물모형혈청고동치、흉선중량비상현저성강저,피질동치비상현저성승고。2）불동시간적유영대만성속박대서산생적효과불동,여모형대조조상비：（1）30 min유영조화60 min유영조적체중、력갈시간의차위비상현저성승고화현저성승고,혈청고동급흉선중량현저성승고,피질동치현저강저;（2）만성속박＋력갈유영조체중、력갈시간、혈청고동화흉선중량유강저추세,피질동유승고추세。3）만성속박가사대서적IFN-γ적함량비상현저성강저;4）만성속박가사대서적IL-4적함량강저,단효과불현저;각조지간무현저성차이。결론：1）만성속박가이성공복제만성피로종합정대서모형。2）30min、60 min유영특별시30 m유영적간예작용가유효예방CFS적발생。3）30 m、60 m유영도가이조절대서FIN-γ적분비,사IFN-γ/IL-4、비솔정향변화,유효예방대서CFS적형성。
Objective： This experiment of swimming research on chronic fatigue syndrome can learn the intervention of chronic fatigue syndrome and the influence of interferon-γ and IL-4.It can provide molecular basis for preventing CFS.Method： 50 eight-week-old male Wister rats were randomly divided into experimental group and control group.And they were killed 24h after the experiment finished.Take 2～3ml centrifuge low blood serum stored at-20 ℃ refrigerator spare blood cortisol and testosterone tested;take 3～4mL centrifuge low blood serum,stored at 4 ℃ refrigerator tested INF-γ and IL-4.Result：1） the value of animal models of serum testosterone and thymus weight,which were copied by chronic restraint,is significantly reduced.Whereas,the value of costerone increased remarkably.2） Different time of swimming has different effect on chronic restraint in rats.Compared with the control group： ①body weight and exhaustive time of 30min and 60min swimming group,extraordinarily increased very much and significantly increased.At the same time,the serum testosterone and thymus weight significantly increased while corticosterone were significantly decreased;② chronic restraint and exhaustive swimming body weight,exhaustive time,serum testosterone and thymus weight get lower but corticosterone tended to increase.3） Chronic restraint can make the content of IFN-γ in rats significantly reduce;4） Chronic restraint can make the content of IL-4 decrease,but the effect was not significant;there is no significant difference between each group.Conclusion：1） The chronic restraint stress can copy the MPTP of CFS successfully.2）the 30min、60min intervene effect of swimming,especially the 30m-swimming,can prevent CFS effectively.3）Both 30m and 60m swimming can adjust rats＇ FIN-γ secretion,make IFN-γ/IL-4 rate change forward and prevent the formation of the rats CFS successfully.
