CAJ | 학술논문

目的：拟从EDN1及NOS2A基因的多态性位点中筛选出与HiHiLo（高住高练低训）训练效果相关的分子标记，为帮助制定个性化的低氧训练方案提供理论参考。方法：采用Association—Study研究方法，测定、分析了72名我国北方地区汉族健康男性青年30天HiHiLo训练前后有氧运动能力、心功能变化率与EDN1、NOS2A基因多态性的关联性。HiHiLo训练方案包括每晚10h低氧环境（14．3％～14．8％含氧量）暴露、每周3次30min75％VO2max强度的低氧运动和日常的体育锻炼。有氧运动能力指标包括递增负荷功率车运动中测定的最大耗氧量、固定负荷血乳酸以及低氧训练中的血氧饱和度，心功能指标包括彩色多普勒测定的安静及三级固定负荷（50W→100W→150w）功率车运动3min后的心脏结构与功能指标。基因多态性选取了EDN1基因外显子SNP／rs5370、rs1800997和3’端rs4714383，NOS2A基因启动子区蛐{（C（肌）n和内含子孙7P／rs2248814。结果：1）经过30天HiH山）训练后，EDNl基因SNP／rsl800997的3A／3A基因型人群在安静状态下左心室心肌收缩力提高程度显著优于含4A等位基因的基因型人群，SNP／rs4714383的CC基因型人群运动中左心室泵功能改善程度显著优于含T等位基因的基因型人群；2）N082A基因STR（GCTTT）12上2个等位基因n值之和与VO2max提高率显著正相关，SNP／rs2779249的GT基因型人群固定负荷运动中心脏机能节省化水平提高的程度显著大于GG基因型人群。结论：在我国北方汉族男性人群中，EDN1基因卧fP／rs1800997、rs4714383和rs2779249的3A／3A、（℃和GT基因型携带者HiHiLo训练后心功能提高效果较好；NOS2A基因STR（CA肼）n基因座上2个等位基因n值均较大的基因型携带者HiHiLo训练后VO2max提高效果较好。
목적：의종EDN1급NOS2A기인적다태성위점중사선출여HiHiLo（고주고련저훈）훈련효과상관적분자표기，위방조제정개성화적저양훈련방안제공이론삼고。방법：채용Association—Study연구방법，측정、분석료72명아국북방지구한족건강남성청년30천HiHiLo훈련전후유양운동능력、심공능변화솔여EDN1、NOS2A기인다태성적관련성。HiHiLo훈련방안포괄매만10h저양배경（14．3％～14．8％함양량）폭로、매주3차30min75％VO2max강도적저양운동화일상적체육단련。유양운동능력지표포괄체증부하공솔차운동중측정적최대모양량、고정부하혈유산이급저양훈련중적혈양포화도，심공능지표포괄채색다보륵측정적안정급삼급고정부하（50W→100W→150w）공솔차운동3min후적심장결구여공능지표。기인다태성선취료EDN1기인외현자SNP／rs5370、rs1800997화3’단rs4714383，NOS2A기인계동자구곡{（C（기）n화내함자손7P／rs2248814。결과：1）경과30천HiH산）훈련후，EDNl기인SNP／rsl800997적3A／3A기인형인군재안정상태하좌심실심기수축력제고정도현저우우함4A등위기인적기인형인군，SNP／rs4714383적CC기인형인군운동중좌심실빙공능개선정도현저우우함T등위기인적기인형인군；2）N082A기인STR（GCTTT）12상2개등위기인n치지화여VO2max제고솔현저정상관，SNP／rs2779249적GT기인형인군고정부하운동중심장궤능절성화수평제고적정도현저대우GG기인형인군。결론：재아국북방한족남성인군중，EDN1기인와fP／rs1800997、rs4714383화rs2779249적3A／3A、（℃화GT기인형휴대자HiHiLo훈련후심공능제고효과교호；NOS2A기인STR（CA정）n기인좌상2개등위기인n치균교대적기인형휴대자HiHiLo훈련후VO2max제고효과교호。
Objective.. To study the association of 5 polymorphisms in EDN1 and NOS2A genes with HiHiLo （living high, exercise high and training low）training response in order to find genetic markers for predicting HiHiLo training effect. Methods： A cohort of 72 young male amateur athletes was recruited to a 30 days HiHiLo training project. The project included exposing to 14.3 ~ 14.8 ~ 02 enviorment 10 hours per day, 30 minutes 7 5 ~//00 VO2,~x intensity ergometer exercise 3 tin：es per week and routine training at sea level as usual. All of the subjects were North Han nationality of China. The phenotype parameters of aerobic capacity and cardiac function were measured before and after HiHiLo training. The aerobic capacity indexes includ VO2~, lactate during 150W exercise and SpOz during hypoxic training. The cardiac function was detected ander rest and during 3-fixed-loads-exercise. Association was observed between the change of phenotype parameters and polymorphisms including SNP/rs5370, rs1800997 and rs4714383 in EDN1 gene, and STR （CCTTT）n and SNP/rs2248814 in NOS2A gene. Main Results： After 30 days of HiHiLo training, 1） the decrease of ESV at rest in EDN1 SNP/rs1800997 3A/3A genotype carriers was significately more than that of 4A allele carri- ers. The increase of EDV during fixed-load exercise in EDN1 SNP/ rs4714383 CC genotype carriers was s：gnificantely higher than that in T allele carriers. 2） The n value of NOS2A STR （CCTTT） n showed signifieantely positive linear correlation with VO2max improvement. The decrease of CO and COI during fixed-load exercise in NOS2A SNP/rs2779249 GT genotype carriers was significantely more than that in GG genotype carriers. Conclusion： Among men of Han Nationality in North China, 1） the 3A/3A, CC and GT genotypes of SNP/rs1800997,rs4714383 and rs2779249 in EDN1 gene are genetic markers for better cardiac function re- sponse to HiHiLo training, 2） The larger n value of STR （CCTTT） n in NOS2A gene is the genetic markers for better VO2max improvement after HiHiLo training.