泉州师范学院学报
泉州師範學院學報
천주사범학원학보
Journal of Quanzhou Normal College
2012年
2期
72~75
,共null页
拟穴青蟹 凝集素 性质
擬穴青蟹 凝集素 性質
의혈청해 응집소 성질
Scylla paramamosain; lectin; properties
采用生物化学的方法研究了拟穴青蟹凝集素的部分性质,结果表明：拟穴青蟹血清的凝集素可凝集兔子、鸽子、鸭子、鸡的动物血红细胞,并且对不同的红细胞的凝集效价有所不同,该凝集素的活性受D-半乳糖、麦芽糖、鼠李糖等3种糖所抑制.在温度为30～40℃,pH为7.0,盐度为24时,拟穴青蟹血清凝集素活性最高.拟穴青蟹血清样品的凝集素受Ca2＋的依赖性较小.
採用生物化學的方法研究瞭擬穴青蟹凝集素的部分性質,結果錶明：擬穴青蟹血清的凝集素可凝集兔子、鴿子、鴨子、鷄的動物血紅細胞,併且對不同的紅細胞的凝集效價有所不同,該凝集素的活性受D-半乳糖、麥芽糖、鼠李糖等3種糖所抑製.在溫度為30～40℃,pH為7.0,鹽度為24時,擬穴青蟹血清凝集素活性最高.擬穴青蟹血清樣品的凝集素受Ca2＋的依賴性較小.
채용생물화학적방법연구료의혈청해응집소적부분성질,결과표명：의혈청해혈청적응집소가응집토자、합자、압자、계적동물혈홍세포,병차대불동적홍세포적응집효개유소불동,해응집소적활성수D-반유당、맥아당、서리당등3충당소억제.재온도위30～40℃,pH위7.0,염도위24시,의혈청해혈청응집소활성최고.의혈청해혈청양품적응집소수Ca2＋적의뢰성교소.
An experimental biochemical method was conducted to investigate the properties of lectin in serum of Scylla paramamosain.The results show that the lectin in serum of Scylla paramamosain can agglutinate erythrocytes of rabbit,dove,duck,chicken,and had a different effects to different erythrocytes.The activity of the agglutinin can be slightly inhibited by D-Galactose,Maltose,Rhamnose.The agglutinin would be highest activity in temperature from 30 to 40 ℃,pH is 7.0,salinity is 24.And the agglutinin is less dependented on Ca2＋.
