北京体育大学学报
北京體育大學學報
북경체육대학학보
Journal of Beijing University of Physical Education
2012年
4期
53~58
,共null页
孙焱 郝选明 耿青青 金焕 张艳艳 任琦
孫焱 郝選明 耿青青 金煥 張豔豔 任琦
손염 학선명 경청청 금환 장염염 임기
apoE基因缺陷小鼠 有氧运动 动脉粥样硬化 细胞因子
apoE基因缺陷小鼠 有氧運動 動脈粥樣硬化 細胞因子
apoE기인결함소서 유양운동 동맥죽양경화 세포인자
apoE-deficient mice; aerobic exercise; atherosclerosis;cytokine
目的:以apoE基因缺陷小鼠建立动脉粥样硬化模型,研究有氧运动对动脉硬化脂质斑块面积和血清相关细胞因子水平的影响。方法:apoE基因缺陷小鼠随机分为安静对照组和运动组(游泳运动,90min/次,6次侗,周末休息,共持续10周),10周后测定和比较两组小鼠的血脂水平、主动脉血管脂质斑块面积以及血清脂联素(adiponectin)、白介素6(IL-6)和肿瘤坏死因子α(TNF-α)的水平。结果:结果表明,与对照组相比,1)有氧运动组小鼠主动脉的脂质斑块面积明显缩小(P〈0.05),血脂状况显著改善。2)10周有氧运动可以显著下调apoE小鼠的TNF-α(P〈0.01)和IL-6(P〈0.01)水平,同时显著上调adiponectin(P〈0.01)的水平。结论:1)10周有氧运动能显著抑制apoE基因缺陷小鼠动脉粥样硬化的发展,并改善动脉粥样硬化小鼠的血脂水平。2)10周有氧运动可以显著提高apoE基因缺陷小鼠血清中抗炎因子的水平,同时显著降低促炎因子的水平,两者综合作用的结果,可有效改善apoE基因缺陷小鼠系统的炎症状态。
目的:以apoE基因缺陷小鼠建立動脈粥樣硬化模型,研究有氧運動對動脈硬化脂質斑塊麵積和血清相關細胞因子水平的影響。方法:apoE基因缺陷小鼠隨機分為安靜對照組和運動組(遊泳運動,90min/次,6次侗,週末休息,共持續10週),10週後測定和比較兩組小鼠的血脂水平、主動脈血管脂質斑塊麵積以及血清脂聯素(adiponectin)、白介素6(IL-6)和腫瘤壞死因子α(TNF-α)的水平。結果:結果錶明,與對照組相比,1)有氧運動組小鼠主動脈的脂質斑塊麵積明顯縮小(P〈0.05),血脂狀況顯著改善。2)10週有氧運動可以顯著下調apoE小鼠的TNF-α(P〈0.01)和IL-6(P〈0.01)水平,同時顯著上調adiponectin(P〈0.01)的水平。結論:1)10週有氧運動能顯著抑製apoE基因缺陷小鼠動脈粥樣硬化的髮展,併改善動脈粥樣硬化小鼠的血脂水平。2)10週有氧運動可以顯著提高apoE基因缺陷小鼠血清中抗炎因子的水平,同時顯著降低促炎因子的水平,兩者綜閤作用的結果,可有效改善apoE基因缺陷小鼠繫統的炎癥狀態。
목적:이apoE기인결함소서건립동맥죽양경화모형,연구유양운동대동맥경화지질반괴면적화혈청상관세포인자수평적영향。방법:apoE기인결함소서수궤분위안정대조조화운동조(유영운동,90min/차,6차동,주말휴식,공지속10주),10주후측정화비교량조소서적혈지수평、주동맥혈관지질반괴면적이급혈청지련소(adiponectin)、백개소6(IL-6)화종류배사인자α(TNF-α)적수평。결과:결과표명,여대조조상비,1)유양운동조소서주동맥적지질반괴면적명현축소(P〈0.05),혈지상황현저개선。2)10주유양운동가이현저하조apoE소서적TNF-α(P〈0.01)화IL-6(P〈0.01)수평,동시현저상조adiponectin(P〈0.01)적수평。결론:1)10주유양운동능현저억제apoE기인결함소서동맥죽양경화적발전,병개선동맥죽양경화소서적혈지수평。2)10주유양운동가이현저제고apoE기인결함소서혈청중항염인자적수평,동시현저강저촉염인자적수평,량자종합작용적결과,가유효개선apoE기인결함소서계통적염증상태。
Objective: ApoE deficient mice was used as AS model to approach the effect and mechanism of aerobic exercise on formation of atherosclerotic lesions and the effect of serum inflammatory - related cytokines. Method: ApoE- deficient mice were randomly divided into control group (CFj) and exercise group (EG, swim- ming, 90rain/d, 6d/w, 10 wks), the lesion area and serum lipid and serum inflammatory - related cytokines were tested after 10 weeks. Result: 1) Compared with CG, the lesion area in the aorta of EG was significantly less than that of the CI3(P〈0.05). And the serum lipid was improved significantly. 2) Compared with CG, the serum levels of TNF -α and IL - 6 were significantly down - regulated in the EG ( P 〈 0.01 ), and the adiponectin level was up - regulated significantly ( P〈 0.01 ). Conclusion: 1 ) The results suggested that aerobic exercise significantly decreased the development of atherosclerotic plaque in apoE deficient mice and simultaneously improved the fat metabolism. 2) Aerobic exercise significantly decreased the proinflammatory cytokine and increased the anti inflammatory cytokine simultaneously in apoE - deficient mice. The systemic inflammation can be effectively improved from aerobic exercise.