中国体育科技
中國體育科技
중국체육과기
China Sport Science and Technology
2012年
3期
141~144
,共null页
李江华 王智慧 朱小娟 葛耀军
李江華 王智慧 硃小娟 葛耀軍
리강화 왕지혜 주소연 갈요군
代谢组学 氯化消毒 核磁共振 模式识别 游泳 鼠 动物实验
代謝組學 氯化消毒 覈磁共振 模式識彆 遊泳 鼠 動物實驗
대사조학 록화소독 핵자공진 모식식별 유영 서 동물실험
metabolomics; chlorination; NMR; pattern recognition; swimming; rat; animal experiment
目的:了解传统氯化消毒方式产生的副产物对游泳训练效果的影响,并利用组织病理学和基于核磁共振(NMR)的代谢组学方法分析这种影响产生的原因及其生理、生化机制。方法:SD雄性大鼠12只,随机分为对照组与实验组各6只,进行为期10周的渐增负荷游泳训练。实验组游泳池水按照普通游泳池水质标准投入次氯酸钙氯化消毒;对照组游泳池水为纯净水。10周训练结束后,首先收集尿样,做代谢组学分析;然后进行负重耐力游泳能力测试,记录大鼠游泳至力竭的时间;测试完成后,大鼠休息2天(48h),断头处死,取肺进行组织病理切片。结果:实验组大鼠游泳至力竭的时间为28.97±2.55min,明显低于对照组的39.15±9.85min(P〈0.05);大部分大鼠肺泡壁出现了毛细血管扩张充血,肺泡腔有水肿液,但是未见器质性病变,实验组和对照组检测结果基本一致,没有明显差异;实验组大鼠代谢表型(1 H NMR代谢指纹图谱)与对照组相比出现了明显的、有群体性特征的变化,其中主要代谢标志物为:γ-氨基丁酸、丙酮酸和马尿酸。结论:按标准剂量对游泳池水投放含氯消毒剂进行氯化消毒没有引起受训大鼠肺部出现器质性病变,但是对耐力游泳训练效果有一定的不良影响,氯化消毒副产物诱发的肝脏解毒功能亢奋和机体代谢表型的变化可能是这一影响产生的主要原因。
目的:瞭解傳統氯化消毒方式產生的副產物對遊泳訓練效果的影響,併利用組織病理學和基于覈磁共振(NMR)的代謝組學方法分析這種影響產生的原因及其生理、生化機製。方法:SD雄性大鼠12隻,隨機分為對照組與實驗組各6隻,進行為期10週的漸增負荷遊泳訓練。實驗組遊泳池水按照普通遊泳池水質標準投入次氯痠鈣氯化消毒;對照組遊泳池水為純淨水。10週訓練結束後,首先收集尿樣,做代謝組學分析;然後進行負重耐力遊泳能力測試,記錄大鼠遊泳至力竭的時間;測試完成後,大鼠休息2天(48h),斷頭處死,取肺進行組織病理切片。結果:實驗組大鼠遊泳至力竭的時間為28.97±2.55min,明顯低于對照組的39.15±9.85min(P〈0.05);大部分大鼠肺泡壁齣現瞭毛細血管擴張充血,肺泡腔有水腫液,但是未見器質性病變,實驗組和對照組檢測結果基本一緻,沒有明顯差異;實驗組大鼠代謝錶型(1 H NMR代謝指紋圖譜)與對照組相比齣現瞭明顯的、有群體性特徵的變化,其中主要代謝標誌物為:γ-氨基丁痠、丙酮痠和馬尿痠。結論:按標準劑量對遊泳池水投放含氯消毒劑進行氯化消毒沒有引起受訓大鼠肺部齣現器質性病變,但是對耐力遊泳訓練效果有一定的不良影響,氯化消毒副產物誘髮的肝髒解毒功能亢奮和機體代謝錶型的變化可能是這一影響產生的主要原因。
목적:료해전통록화소독방식산생적부산물대유영훈련효과적영향,병이용조직병이학화기우핵자공진(NMR)적대사조학방법분석저충영향산생적원인급기생리、생화궤제。방법:SD웅성대서12지,수궤분위대조조여실험조각6지,진행위기10주적점증부하유영훈련。실험조유영지수안조보통유영지수질표준투입차록산개록화소독;대조조유영지수위순정수。10주훈련결속후,수선수집뇨양,주대사조학분석;연후진행부중내력유영능력측시,기록대서유영지력갈적시간;측시완성후,대서휴식2천(48h),단두처사,취폐진행조직병리절편。결과:실험조대서유영지력갈적시간위28.97±2.55min,명현저우대조조적39.15±9.85min(P〈0.05);대부분대서폐포벽출현료모세혈관확장충혈,폐포강유수종액,단시미견기질성병변,실험조화대조조검측결과기본일치,몰유명현차이;실험조대서대사표형(1 H NMR대사지문도보)여대조조상비출현료명현적、유군체성특정적변화,기중주요대사표지물위:γ-안기정산、병동산화마뇨산。결론:안표준제량대유영지수투방함록소독제진행록화소독몰유인기수훈대서폐부출현기질성병변,단시대내력유영훈련효과유일정적불량영향,록화소독부산물유발적간장해독공능항강화궤체대사표형적변화가능시저일영향산생적주요원인。
Objective: To explore the influence of the chlorinated pool water to swimming training effects and analyze the biological mechanism which makes this influence happen.Method:Twelve male SD rats were randomly separated into experimental group and control group,an increasing load swimming training was carried out for ten weeks.The pool water of the experimental group was disinfected with calcium hypochlorite,while the pool water used for the control group was purified with a water purifier.After ten weeks training,the urine samples were collected for metabolomics analysis,and then a swimming capacity test was taken by recording the swimming time to exhaustion of each rat.Two days(48 h) later,all rats were decapitated and their lungs were collected for histopathology analysis.Results:The exhaustion time of experimental group was 28.97±2.55 minutes,which is significantly lower than the control group's 39.15±9.85 minutes(P0.05);No significant differences were found between two groups in the histopathology analysis.There were no organic disease appearing,but the capillary vessels around alveolus of both groups expanded and congested.Compared to the control group,the metabolic phenotype of experimental group significantly changed,and the main metabolic biomarkers were hippurate,pyruvate,γ-amino-n-butyrate.Conclusion:Chlorination of the swimming pool water with standard dose doesn't induce organic disease to the trained rats,but it does impair the training effects.Liver detoxification function excitement and the changed metabolic phenotype,induced by disinfection byproducts,might be the main factors for this influence.