CAJ | 학술논문

为了研究肉碱棕榈酰转移酶（CPT-1β）基因区的rs131758、rs470117多态位点以及由其组成的单体型与有氧耐力训练前后敏感性指标的变化之间的关联性，探讨其作为有氧耐力训练敏感性标记的可行性，对102名中国北方汉族青年进行为期18周的有氧耐力训练。采用活动跑台的逐级递增运动负荷方法测定训练前后“跑节省化”（RE）相关指标；采用卧式蹬车的极限下递增定量负荷运动方式测定训练前后不同负荷状态下（安静时、50W、100W、150W、恢复期）的心脏结构指标；由心脏结构指标获得心脏功能相关指标。采用MALDI-TOF MS方法解析受试者基因组DNArs131758、rs470117多态位点基因型。分析训练前后相关指标的变化与多态位点不同基因型之间的关联性。结果发现：1）rs131758位点的AA型与训练后跑节省化时心率（△HRRE）的训练敏感性相关联；2）rs131758位点的AA型与训练后心脏收缩末期左室后壁厚度△PWS指标的训练敏感性相关联；3）由rs131758-rs470117位点组成的单体型AT与训练后安静时每搏输出量指数（ASVI）、心动周期（△T）、心输出量指数（ACOI）的训练敏感性相关联。说明：rs131758位点的AA型和由rs131758-rs470117位点组成的AT单体型可以作为中国北方汉族人群对有氧耐力训练敏感的分子标记。
위료연구육감종려선전이매（CPT-1β）기인구적rs131758、rs470117다태위점이급유기조성적단체형여유양내력훈련전후민감성지표적변화지간적관련성，탐토기작위유양내력훈련민감성표기적가행성，대102명중국북방한족청년진행위기18주적유양내력훈련。채용활동포태적축급체증운동부하방법측정훈련전후“포절성화”（RE）상관지표；채용와식등차적겁한하체증정량부하운동방식측정훈련전후불동부하상태하（안정시、50W、100W、150W、회복기）적심장결구지표；유심장결구지표획득심장공능상관지표。채용MALDI-TOF MS방법해석수시자기인조DNArs131758、rs470117다태위점기인형。분석훈련전후상관지표적변화여다태위점불동기인형지간적관련성。결과발현：1）rs131758위점적AA형여훈련후포절성화시심솔（△HRRE）적훈련민감성상관련；2）rs131758위점적AA형여훈련후심장수축말기좌실후벽후도△PWS지표적훈련민감성상관련；3）유rs131758-rs470117위점조성적단체형AT여훈련후안정시매박수출량지수（ASVI）、심동주기（△T）、심수출량지수（ACOI）적훈련민감성상관련。설명：rs131758위점적AA형화유rs131758-rs470117위점조성적AT단체형가이작위중국북방한족인군대유양내력훈련민감적분자표기。
In order to study the correlations between polymorphic loci rs 131758 and rs 470117 in the camitine palmityl transferase-1β （CPT-1β） gene region, as well as the haplotype they form, and the changes of sensitivity indexes before and after aerobic endurance training, and to probed into the feasibility to use them as sensitivity mark- ers for aerobic endurance training, the authors carried out 18-week aerobic endurance training on 102 youngsters of the Han nationality in northern China, measured ＂running economization＂（RE） related indexes before and after training by using the method of gradually increasing treadmill exercise load, and cardiac structural indexes under different load conditions （at calm, 50W, 100W, 150W, in the recovery period） before and after training by adopting the way of exercising with gradually increased quantitative load under the limit of layback style riding, acquired cardiac function related indexes from cardiac structural indexes, dissected the genotypes of polymorphic loci rs 131758 and rs 470117 of genome DNA of the testees by using the MALDI-TOF MS method, analyzed the correlations between the changes of relevant indexes before and after training and the different genotypes of polymorphic loci, and revealed the following findings： 1）type AA of locus rs 131758 was correlative to the training sensitivity of heart rate （AHRRE） at running economization after training; 2）type AA of locus rs131758 was correlative to the training sensitivity of left ventricular rear wall thickness （APWS） at the end of systole after training; 3）haplotype AT formed by loci rs 131758 and rs 470117 was correlative to the training sensitivity of stroke output volume index （ASVI）, cardiac cycle （AT） and cardiac output index （ACOI） at calm after training. The said findings indicate that type AA of locus rs 131758 and haplotype AT formed by loci rs 131758 and rs 470117 can be used as molecular markers for aerobic endurance training for people of the Nan nationality in northern China.