体育学刊
體育學刊
체육학간
Journal of Physical Education
2012年
3期
140~144
,共null页
运动生物化学 热激蛋白70 高温预处理 海马区 自由基 Ca^2-ATP酶 大鼠
運動生物化學 熱激蛋白70 高溫預處理 海馬區 自由基 Ca^2-ATP酶 大鼠
운동생물화학 열격단백70 고온예처리 해마구 자유기 Ca^2-ATP매 대서
sports biochemistry; heat stress protein 70; high temperature pretreatment; hippocampus area; free radical; Ca^2+-ATPase; rats
探讨高温预处理与不同强度的一次性急性运动共同干预下所诱导的热休克蛋白70,对大鼠海马区自由基以及钙ATP酶的影响。将3月龄SD大鼠48只,分为室温安静组、室温15m/min运动组、室温27m/min运动组,高温安静组、高温15m/min运动组和高温27m/min运动组等6组,每组8只。高温组大鼠经高温预处理24h后,与其他运动组进行一次性急性运动60min后即刻取材检测海马区热休克蛋白Hsp70、丙二醛(MDA)含量,超氧化物歧化酶SOD、Ca^2+-ATPase活性。结果发现室温27m/min运动组较之室温安静组,其大鼠海马区Hsp70的表达水平明显升高(P〈0.05);高温预处理各组的Hsp70表达水平,显著高于室温各组(P〈0.05);与室温运动组比较,高温预处理运动组海马SOD的活性提高极明显(P〈0.01);各高温组MDA含量随运动强度增大而降低,但组间差异无显著性(P〉0.05);高温预处理各组Ca^2+-ATPase活性均显著高于室温各组(P〈0.05)。结果说明:高温预处理诱导Hsp70的大量表达可提高运动时海马的SOD活性,降低MDA含量,提高Ca^2+-ATPase活性。提示Hsp70可能通过提高抗氧化能力,以减少自由基对海马区的损害,对神经系统具有一定的保护意义。
探討高溫預處理與不同彊度的一次性急性運動共同榦預下所誘導的熱休剋蛋白70,對大鼠海馬區自由基以及鈣ATP酶的影響。將3月齡SD大鼠48隻,分為室溫安靜組、室溫15m/min運動組、室溫27m/min運動組,高溫安靜組、高溫15m/min運動組和高溫27m/min運動組等6組,每組8隻。高溫組大鼠經高溫預處理24h後,與其他運動組進行一次性急性運動60min後即刻取材檢測海馬區熱休剋蛋白Hsp70、丙二醛(MDA)含量,超氧化物歧化酶SOD、Ca^2+-ATPase活性。結果髮現室溫27m/min運動組較之室溫安靜組,其大鼠海馬區Hsp70的錶達水平明顯升高(P〈0.05);高溫預處理各組的Hsp70錶達水平,顯著高于室溫各組(P〈0.05);與室溫運動組比較,高溫預處理運動組海馬SOD的活性提高極明顯(P〈0.01);各高溫組MDA含量隨運動彊度增大而降低,但組間差異無顯著性(P〉0.05);高溫預處理各組Ca^2+-ATPase活性均顯著高于室溫各組(P〈0.05)。結果說明:高溫預處理誘導Hsp70的大量錶達可提高運動時海馬的SOD活性,降低MDA含量,提高Ca^2+-ATPase活性。提示Hsp70可能通過提高抗氧化能力,以減少自由基對海馬區的損害,對神經繫統具有一定的保護意義。
탐토고온예처리여불동강도적일차성급성운동공동간예하소유도적열휴극단백70,대대서해마구자유기이급개ATP매적영향。장3월령SD대서48지,분위실온안정조、실온15m/min운동조、실온27m/min운동조,고온안정조、고온15m/min운동조화고온27m/min운동조등6조,매조8지。고온조대서경고온예처리24h후,여기타운동조진행일차성급성운동60min후즉각취재검측해마구열휴극단백Hsp70、병이철(MDA)함량,초양화물기화매SOD、Ca^2+-ATPase활성。결과발현실온27m/min운동조교지실온안정조,기대서해마구Hsp70적표체수평명현승고(P〈0.05);고온예처리각조적Hsp70표체수평,현저고우실온각조(P〈0.05);여실온운동조비교,고온예처리운동조해마SOD적활성제고겁명현(P〈0.01);각고온조MDA함량수운동강도증대이강저,단조간차이무현저성(P〉0.05);고온예처리각조Ca^2+-ATPase활성균현저고우실온각조(P〈0.05)。결과설명:고온예처리유도Hsp70적대량표체가제고운동시해마적SOD활성,강저MDA함량,제고Ca^2+-ATPase활성。제시Hsp70가능통과제고항양화능력,이감소자유기대해마구적손해,대신경계통구유일정적보호의의。
In order to probe into the influence of heat shock protein 70 induced by the joint intervention of high temperature pretreatment and one-time acute exercising with different intensities on flee radicals and Ca^ 2+-ATPase in the hippocampus area of rats, the authors divided 48 SD 3-month old rats into 6 groups: a room temperature calm group, a room temperature 15m/min exercising group, a room temperature 27m/min exercising group, a high temperature calm group, a high temperature 15m/min exercising group, and a high temperature 27m/min exercising group. 24h after the rats in high temperature groups (8 in each group) were pretreated by high temperature, they did a 60min one-time acute exercise together with the rats in other exercising groups, and immediately after that, hot shock protein Hsp70 and MDA contents as well as SOD and Ca^ 2+-ATPase activities in the hippocampus were sam- ple taken and tested. The results were as follows: comparing the rats in the room temperature 27m/min exercising group with the rats in the room temperature calm group, the level of expression of Hsp70 in the hippocampus area rose significantly (P〈0.05); the levels of expression of Hsp70 of the rats in groups pretreated by high temperature were significantly higher than those of the rats in room temperature groups (P〈0.05); as compared with the rats in room temperature exercising groups, the SOD activity of hippocampus of the rates in exercising groups pretreated by high temperature increased extremely significantly (P〈0.01); the MDA contents of the rats in high temperature groups decreased as exercise intensity increased, but showed no significant difference; the Ca^ 2+-ATPase activities of the rats in groups pretreated by high temperature were all significantly higher than those of the rats in room temperature groups (P〈0.05). The results indicated the followings: a lot of Hsp70 expression induced by high temperature pretreatment can boost the SOD activity of hippocampus during exercising, lower MDA content, and boost Ca^ 2+-ATPase activity. It is suggested that Hsp70 can reduce the damage to the hippocampus area cased by free radi- cals by enhancing antioxidation ability, thus it is provided with certain significance in protecting the nervous system.