CAJ | 학술논문

微生物是影响古代壁画安全保存与展示的一个重要因素,其检测是壁画保护研究的重要环节。分子生物学技术通过直接分析微生物遗传物质确定种属,可以避免传统检测技术必需的微生物培养过程,不仅快速、全面,更重要的是能够确定主要致病菌。本研究用分子生物学技术检测中国北方两个5世纪壁画墓,确定导致壁画菌害的主要种群为假诺卡氏菌（Pseudonocardia sp.）,为微生物病害治理和预防提供了基本依据。
미생물시영향고대벽화안전보존여전시적일개중요인소,기검측시벽화보호연구적중요배절。분자생물학기술통과직접분석미생물유전물질학정충속,가이피면전통검측기술필수적미생물배양과정,불부쾌속、전면,경중요적시능구학정주요치병균。본연구용분자생물학기술검측중국북방량개5세기벽화묘,학정도치벽화균해적주요충군위가낙잡씨균（Pseudonocardia sp.）,위미생물병해치리화예방제공료기본의거。
Microorganisms are a major concern in preservation and exhibition of ancient mural paintings. Their identification is a major step leading toward better conservation. Compared with traditional methods, molecular biol- ogy - based techniques directly analyze the genetic materials of the microorganisms, avoiding the culturing process and providing a fast and comprehensive method for the identification of major pathogens. Molecular biological techniques were used to detect microorganisms in two tombs built in the 5th century in North China. Pseudonocardia species as the main pathogens was identified. Results provided important information for planning the treatment and prevention of mural damage caused by microorganisms.