CAJ | 학술논문

目的：建立高效液相色谱法测定二十五味珊瑚丸中西红花苷含量的方法。方法：采用HPLC法，色谱柱：DionexC18柱（250ram×4．60mm，5um）；流动相为甲醇-水（52：48），流速为0．8ml／min，检测波长为440nm，柱温为250C；进样量10μl；用标准曲线法定量，测定二十五味珊瑚丸中西红花苷含量。结果：西红花苷I在33μg-330μg范围内与峰面积呈良好的线性关系（r=0．9999），西红花苷Ⅱ在62μg-310μg范围内与峰面积呈良好的线性关系（r=0．9998），平均回收率为103．73％，RSD为1．68％（n=6）。结论：本方法操作简单、重现性好，可用于测定二十五味珊瑚丸中西红花苷I＆Ⅱ含量。
목적：건립고효액상색보법측정이십오미산호환중서홍화감함량적방법。방법：채용HPLC법，색보주：DionexC18주（250ram×4．60mm，5um）；류동상위갑순-수（52：48），류속위0．8ml／min，검측파장위440nm，주온위250C；진양량10μl；용표준곡선법정량，측정이십오미산호환중서홍화감함량。결과：서홍화감I재33μg-330μg범위내여봉면적정량호적선성관계（r=0．9999），서홍화감Ⅱ재62μg-310μg범위내여봉면적정량호적선성관계（r=0．9998），평균회수솔위103．73％，RSD위1．68％（n=6）。결론：본방법조작간단、중현성호，가용우측정이십오미산호환중서홍화감I＆Ⅱ함량。
The content of crocin in the 25 odors Coral pill were quantitatively measured with standard curve by using HPLC （chromatographic column： Dionex C18 （250mm×4.60mm, 5urn）; mobile phrase consisted of MeOH-water （52：48）;flow rate： 0.8ml/min; detection wavelength： 440nm; column temperature： 25%; sample load： 10μl）to establish a determining method of the crocin [ ＆ Ⅱ in the 25 odors Coral pill. The results showed that there is a good linear relationship between peak area and in the range of 33-330μg （r=0.9999） of crocin and between peak area in the range of 62-310μg （r=0.9998） of crocin at a mean recovery rate of 103.73% and 1.68% of RSD （n=6）. In conclusion, this method could be used to determine the crocin in the 25 odors Coral pill with simple operation and good repeatability,