CAJ | 학술논문

目的观察晚期运动预适应保护效应中大鼠心肌αPKC表达和分布的变化,探讨该变化的作用及机制。方法将SD大鼠随机分为对照组（C组）、力竭组（EE组）、晚期运动预适应组（LEP组）和晚期运动预适应＋力竭组（LEP＋EE组）。采用一次间歇性大强度跑台运动建立运动预适应动物模型,采用一次力竭跑台运动建立力竭动物模型。运用蛋白质印迹法,定量检测大鼠心肌组织αPKC和p-αPKC表达水平;运用免疫组织化学法,定位检测大鼠心肌细胞αPKC和p-αPKC的分布。结果 1）蛋白质印迹。①αPKC：与C组比较,EE组和LEP组αPKC表达水平显著升高（P〈0.05）;与EE组比较,LEP＋EE组显著降低（P〈0.05）。②p-αPKC：与C组比较,EE组p-αPKC表达水平显著升高（P〈0.05）;与EE组比较,LEP＋EE组显著降低（P〈0.05）。2）免疫组织化学。①αPKC：免疫阳性产物呈棕黄色,以颗粒状弥散分布在胞质中或聚集在闰盘处;各组差异在于颜色有深有浅,颗粒或大或小。②p-αPKC：免疫阳性产物呈棕黄色,C组和LEP＋EE组分布在胞质中;除上述现象外,EE组有细胞膜聚集和核染现象,LEP组出现细胞膜聚集现象。结论αPKC参与了LEP效应对力竭运动大鼠心肌的保护作用,在该作用中αPKC表现为表达水平和激活水平均下调与定位改变。
목적관찰만기운동예괄응보호효응중대서심기αPKC표체화분포적변화,탐토해변화적작용급궤제。방법장SD대서수궤분위대조조（C조）、력갈조（EE조）、만기운동예괄응조（LEP조）화만기운동예괄응＋력갈조（LEP＋EE조）。채용일차간헐성대강도포태운동건립운동예괄응동물모형,채용일차력갈포태운동건립력갈동물모형。운용단백질인적법,정량검측대서심기조직αPKC화p-αPKC표체수평;운용면역조직화학법,정위검측대서심기세포αPKC화p-αPKC적분포。결과 1）단백질인적。①αPKC：여C조비교,EE조화LEP조αPKC표체수평현저승고（P〈0.05）;여EE조비교,LEP＋EE조현저강저（P〈0.05）。②p-αPKC：여C조비교,EE조p-αPKC표체수평현저승고（P〈0.05）;여EE조비교,LEP＋EE조현저강저（P〈0.05）。2）면역조직화학。①αPKC：면역양성산물정종황색,이과립상미산분포재포질중혹취집재윤반처;각조차이재우안색유심유천,과립혹대혹소。②p-αPKC：면역양성산물정종황색,C조화LEP＋EE조분포재포질중;제상술현상외,EE조유세포막취집화핵염현상,LEP조출현세포막취집현상。결론αPKC삼여료LEP효응대력갈운동대서심기적보호작용,재해작용중αPKC표현위표체수평화격활수평균하조여정위개변。
The paper observes the change of expression and distribution of αPKC in the myocardium of rat during the cardio-protective effect of the late exercise preconditioning and explores the function and mechanism of the change.Methods： Rats were randomly assigned to C group,EE group,LEP group and LEP＋EE group.The rat model of EP was set up by means of the single bout of intermittent high-intensity treadmill exercise,and the rat model of exhaustion in the manner of exhaustive treadmill exercise.Western Blot was introduced to quantitatively determine the content of αPKC and p-αPKC in the myocardium of rat.Immuno-histochemistry method was used to determine the distribution of αPKC and p-αPKC.Results： 1） protein mark.① αPKC： compared with C group,there is a significant increase of expression distribution for EE group and LEP group（P〈0.05）;compared with EE group,there is a significant decrease for LEP＋EE（P〈0.05）.② p-αPKC： compared with C group,there is a significant increase of expression level for EE group（P〈0.05）;compared with EE group,there is a significant decrease for LEP＋EE（P〈0.05）.2） Immuno-histochemistry.① αPKC： immune positive products appear orange yellow and scatter in cytoplast or gather around intercalated disc in the form of particles,with the different shades of color and particles in each group.② p-αPKC： immune positive products appear orange yellow and scatter in cytoplast in C group and LEP＋EE group.Besides,they gather around cytomembrane or appear on it.Conclusion： αPKC plays a role in the myocardium of rat during the cardioprotection of LEP effect in EE,which shows the downturn of both expression level and stimulation level and the change of orientation.