北京体育大学学报
北京體育大學學報
북경체육대학학보
Journal of Beijing University of Physical Education
2012年
7期
52~57
,共null页
崔建梅 药宏亮 刘鸿宇 薛金娟
崔建梅 藥宏亮 劉鴻宇 薛金娟
최건매 약굉량 류홍우 설금연
雪芙蓉胶囊 运动能力 雄激素 乳酸 伏隔核 c-fos
雪芙蓉膠囊 運動能力 雄激素 乳痠 伏隔覈 c-fos
설부용효낭 운동능력 웅격소 유산 복격핵 c-fos
Xuefurong capsule; sports ability; testosterone; lactic acid; nucleus accumbens; c-fos
目的：观察中药雪芙蓉胶囊对雄性大鼠力竭跑台时间、血清雄激素及乳酸水平、血浆pH值及大脑伏隔核c-fos表达的影响。方法：将30只雄性成年大鼠随机分为3组,即安静对照组、训练组、中药训练组。中药训练组每天灌服雪芙蓉胶囊（5 mg/kg）,8周后测试大鼠力竭跑台时间,检测血清睾酮、乳酸含量及血浆pH值,用免疫组织化学方法结合图像半定量方法对c-fos神经元的数量、面积及灰度进行测量和分析。结果：与训练组比较,中药训练组大鼠力竭跑台时间延长、血清睾酮及血浆pH增高（P〈0.01,P〈0.05,P〈0.05）、血清乳酸下降（P〈0.05）;伏隔核c-fos免疫阳性细胞数量和阳性产物面积表达均降低（P〈0.05）,灰度没有显著差异（P〉0.05）。结论：中药雪芙蓉胶囊可提高大鼠的运动能力,延缓中枢疲劳,机理可能与此中药提高力竭运动大鼠血清睾酮水平及血浆pH值,同时降低大鼠血清乳酸水平及大脑伏隔核c-fos的过度表达有关。
目的：觀察中藥雪芙蓉膠囊對雄性大鼠力竭跑檯時間、血清雄激素及乳痠水平、血漿pH值及大腦伏隔覈c-fos錶達的影響。方法：將30隻雄性成年大鼠隨機分為3組,即安靜對照組、訓練組、中藥訓練組。中藥訓練組每天灌服雪芙蓉膠囊（5 mg/kg）,8週後測試大鼠力竭跑檯時間,檢測血清睪酮、乳痠含量及血漿pH值,用免疫組織化學方法結閤圖像半定量方法對c-fos神經元的數量、麵積及灰度進行測量和分析。結果：與訓練組比較,中藥訓練組大鼠力竭跑檯時間延長、血清睪酮及血漿pH增高（P〈0.01,P〈0.05,P〈0.05）、血清乳痠下降（P〈0.05）;伏隔覈c-fos免疫暘性細胞數量和暘性產物麵積錶達均降低（P〈0.05）,灰度沒有顯著差異（P〉0.05）。結論：中藥雪芙蓉膠囊可提高大鼠的運動能力,延緩中樞疲勞,機理可能與此中藥提高力竭運動大鼠血清睪酮水平及血漿pH值,同時降低大鼠血清乳痠水平及大腦伏隔覈c-fos的過度錶達有關。
목적：관찰중약설부용효낭대웅성대서력갈포태시간、혈청웅격소급유산수평、혈장pH치급대뇌복격핵c-fos표체적영향。방법：장30지웅성성년대서수궤분위3조,즉안정대조조、훈련조、중약훈련조。중약훈련조매천관복설부용효낭（5 mg/kg）,8주후측시대서력갈포태시간,검측혈청고동、유산함량급혈장pH치,용면역조직화학방법결합도상반정량방법대c-fos신경원적수량、면적급회도진행측량화분석。결과：여훈련조비교,중약훈련조대서력갈포태시간연장、혈청고동급혈장pH증고（P〈0.01,P〈0.05,P〈0.05）、혈청유산하강（P〈0.05）;복격핵c-fos면역양성세포수량화양성산물면적표체균강저（P〈0.05）,회도몰유현저차이（P〉0.05）。결론：중약설부용효낭가제고대서적운동능력,연완중추피로,궤리가능여차중약제고력갈운동대서혈청고동수평급혈장pH치,동시강저대서혈청유산수평급대뇌복격핵c-fos적과도표체유관。
Objective： To explore the effects of Xuefurong capsule on the time of exhausted treadmill, the level of serum testosterone,lactic acid （LA）, the PH of blood plasma, and expression of c-fos within nucleus aeeumbens （Nac） Of brain in the male rats. Method： 30 SD rats were randomly divided into 3 groups： the sedentary control group,the training group,and the Chinese-medicine and training group. The Chinese-medicine and training group were forced to take Xuefurong capsule every day （5mg/kg） and lasted 8 weeks. Then the time of exhausted treadmill of rats was tested, the level of serum testosterone, the LA and the PH of blood plasma were examined, c-los expression within Nac was detected and analysised by using ABC immunohistochemical technique and semi quantitative. Result： Compared with the training group, the time of exhausted treadmill was prolonged（P 〈 0.01 ）, the level of testosterone and PH of blood plasma increased （ P 〈 0.05, P 〈 0.05）, the level of LA de- creased（P 〈 0.05） ; The amount and area of c-fos within Nac obviously decreased（ P 〈 0.05）, the gray degree was not significant different（ P 〉 0.05） in the Chinese medicine and training group. Conclusion： Taking Xue- furong capsules may enhance the sports ability, its mechanism might be that Xuefurong capsule increase the level of serum testosterone and PH blood of plasma, decrease the level of serum LA and excessive expression of c-fos within Nac at the same time.
