广州体育学院学报
廣州體育學院學報
엄주체육학원학보
Journal of Guangzhou Physical Education Institute
2012年
5期
102~107
,共null页
徐锋鹏 胡敏 宋吉锐 林文搜 盛佳智
徐鋒鵬 鬍敏 宋吉銳 林文搜 盛佳智
서봉붕 호민 송길예 림문수 성가지
游泳 红细胞生成 造血微环境 成骨细胞 脂肪细胞
遊泳 紅細胞生成 造血微環境 成骨細胞 脂肪細胞
유영 홍세포생성 조혈미배경 성골세포 지방세포
swimming exercise ; erythropoiesis ; hematological microenvironments ; osteob|ast ; adipocyte
探讨12周游泳运动对大鼠骨髓造血微环境及红细胞生成能力的影响,为运动提高骨髓造血能力提供理论依据。4周龄SD大鼠16只随机分为运动组和对照组。运动组进行12周递增负荷游泳运动,干预期后经腹主动脉取外周血测试血常规;取股骨测量骨髓红系爆式集落形成单位(BFU—E)、成纤维样集落形成单位(CFU—F)及成骨成脂诱导分化能力;取胫骨近侧干骺端制作HE切片,观测脂肪细胞面积。结果表明,和对照组相比,12周游泳运动使大鼠骨髓BFU—E集落数明显增多(P〈0.05);骨髓成骨诱导茜素红s染色OD值明显增大,成脂分化油红O染色OD值明显减小(均P〈0.05);同时胫骨HE切片脂肪细胞面积明显减少(P〈0.05)。结论:(1)游泳运动可以促进大鼠骨髓成骨细胞分化,减少脂肪细胞分化,改善造血微环境;(2)游泳运动促进骨髓红系造血能力。
探討12週遊泳運動對大鼠骨髓造血微環境及紅細胞生成能力的影響,為運動提高骨髓造血能力提供理論依據。4週齡SD大鼠16隻隨機分為運動組和對照組。運動組進行12週遞增負荷遊泳運動,榦預期後經腹主動脈取外週血測試血常規;取股骨測量骨髓紅繫爆式集落形成單位(BFU—E)、成纖維樣集落形成單位(CFU—F)及成骨成脂誘導分化能力;取脛骨近側榦骺耑製作HE切片,觀測脂肪細胞麵積。結果錶明,和對照組相比,12週遊泳運動使大鼠骨髓BFU—E集落數明顯增多(P〈0.05);骨髓成骨誘導茜素紅s染色OD值明顯增大,成脂分化油紅O染色OD值明顯減小(均P〈0.05);同時脛骨HE切片脂肪細胞麵積明顯減少(P〈0.05)。結論:(1)遊泳運動可以促進大鼠骨髓成骨細胞分化,減少脂肪細胞分化,改善造血微環境;(2)遊泳運動促進骨髓紅繫造血能力。
탐토12주유영운동대대서골수조혈미배경급홍세포생성능력적영향,위운동제고골수조혈능력제공이론의거。4주령SD대서16지수궤분위운동조화대조조。운동조진행12주체증부하유영운동,간예기후경복주동맥취외주혈측시혈상규;취고골측량골수홍계폭식집락형성단위(BFU—E)、성섬유양집락형성단위(CFU—F)급성골성지유도분화능력;취경골근측간후단제작HE절편,관측지방세포면적。결과표명,화대조조상비,12주유영운동사대서골수BFU—E집락수명현증다(P〈0.05);골수성골유도천소홍s염색OD치명현증대,성지분화유홍O염색OD치명현감소(균P〈0.05);동시경골HE절편지방세포면적명현감소(P〈0.05)。결론:(1)유영운동가이촉진대서골수성골세포분화,감소지방세포분화,개선조혈미배경;(2)유영운동촉진골수홍계조혈능력。
The purpose of present study was to examine whether 12 weeks of swimming exercise could improve the rats" medul- lary hematological microenvironments and enhance the erythroid progenitors proliferation ability. The 16 Sprague - Dawley rats, 4 weeks old, were randomly assigned to control group and swimming group, each with 8 rats. The swimming group underwent 12 weeks progressive swimming program, with 90 minutes per day, 6 days per week. At the end of the intervention, the rats were sacrificed under urethane, blood was drawn from abdominal aorta for regular test, both femurs and the left tibia were re- moved for bone marrow cell culture or histology analysis. The results showed that 12 weeks swimming had a significant role on the proliferation ability of the erythroid progenitors, with the BFU - E number increased significantly, compared with the con- trol group ( p 〈 O. 05 ). The osteoblast differentiation of bone marrow mesenchymal stem cell ( MSC ) was enhanced in the swimming group, while the adipocyte differentiation was reduced significantly, compared with the control group ( both p 〈 O. 05, respectively). At the same time, decreased adiposity was also observed in the proximal tibia metaphysis of the swimming rats. In conclusion, swimming exercise promotes erythropoiesis and does so through improvements in medullary hematological microenvironments.