CAJ | 학술논문

目的：建立SD大鼠运动预处理模型和一次性力竭运动模型，通过观察大鼠大脑皮质微结构、超微结构的变化以及指标测试，探讨运动预处理对一次性力竭后大脑皮质缺血再灌注的保护机制，为科学地进行运动训练提供神经生物学参考依据。方法：选用雄性4月龄SD大鼠70只，适应性喂养1周后随机分为：空白对照组（C组），一次性力竭组（E组）和运动预处理组（EP组），C组、E组大鼠常规喂养6周，EP组采用间歇训练法运动预处理6周。6周后除c组外，其余组大鼠采用一次性力竭训练，并分别在即刻、24小时、48小时取材。测定大鼠脑组织SOD、GSH—PX、MDA；制作切片观察不同时间各组脑组织微结构和超微结构变化。结果：①自由基检测结果：各时相点大鼠脑组织EP组SOD，GSH—PX活性均显著性高于C组和E组（P〈0．05）；其中EP组在即刻较其它组出现非常显著性（P〈0．01），而在第2个时相点E组和EP组均出现非常显著性下降（P〈0．01），在第3个时相点出现显著性上升（P〈0．05）；E组和EP组之间MDA含量没有显著性差（P〉0．05），但是E组MDA含量却高于EP组②大鼠大脑皮质微结构和超微结构结果：EP组各时相点神经元细胞核损伤、线粒体损伤均明显好于E组。结论：运动预处理可提高力竭运动后大脑组织SOD、GSH—PX活性，减轻脂质过氧化程度，提高大脑清除自由基能力，从而有效预防一次性力竭运动造成的脑缺血再灌注损伤，起到对机体的内源性保护作用。
목적：건립SD대서운동예처리모형화일차성력갈운동모형，통과관찰대서대뇌피질미결구、초미결구적변화이급지표측시，탐토운동예처리대일차성력갈후대뇌피질결혈재관주적보호궤제，위과학지진행운동훈련제공신경생물학삼고의거。방법：선용웅성4월령SD대서70지，괄응성위양1주후수궤분위：공백대조조（C조），일차성력갈조（E조）화운동예처리조（EP조），C조、E조대서상규위양6주，EP조채용간헐훈련법운동예처리6주。6주후제c조외，기여조대서채용일차성력갈훈련，병분별재즉각、24소시、48소시취재。측정대서뇌조직SOD、GSH—PX、MDA；제작절편관찰불동시간각조뇌조직미결구화초미결구변화。결과：①자유기검측결과：각시상점대서뇌조직EP조SOD，GSH—PX활성균현저성고우C조화E조（P〈0．05）；기중EP조재즉각교기타조출현비상현저성（P〈0．01），이재제2개시상점E조화EP조균출현비상현저성하강（P〈0．01），재제3개시상점출현현저성상승（P〈0．05）；E조화EP조지간MDA함량몰유현저성차（P〉0．05），단시E조MDA함량각고우EP조②대서대뇌피질미결구화초미결구결과：EP조각시상점신경원세포핵손상、선립체손상균명현호우E조。결론：운동예처리가제고력갈운동후대뇌조직SOD、GSH—PX활성，감경지질과양화정도，제고대뇌청제자유기능력，종이유효예방일차성력갈운동조성적뇌결혈재관주손상，기도대궤체적내원성보호작용。
Objective ： In this study the establishment of SD rat model and a one - time pre - movement model of exhaustive exercise, by observing the micro - structure of rat cerebral cortex, ultrastructur- al changes, and indicators of exercise tests on a one - time pre - exhausted cerebral cortex caused by lack of blood- reperfusion injury protection mechanisms, in order to provide a scientific reference for exercise training. After adaptive feeding for 1 week,70 four - month - old SD male rats were randomly divided into three major groups ： the control group （ C group）, a one - time exhaustive group （E group）, exercise preconditioning group （EP group）. C group and E group were fed 6 weeks routinely, while EP group were pretreated 6 weeks by intermittent training. 6 weeks later, all groups except C group took an exhaustive swimming training, and separately selected sampling in- stantly, 24 hours, 48 hours after training. SOD, GSH - PX activity and MDA levels of rats cerebral cortex were detected, and rat cerebral cortex micro -structure and ultrastructure observed in defer- ent time by sections. Results：（2）Compared with C and E group, SOD and GSH -PX activity of EP group were obviously higher at deferent time points, especially very significantly higher than the oth- er groups （P 〈0.01）at instant time. While SOD and GSH- PX activity of E and EP group de- creased significantly at the second time point （P 〈0.01 ） and obviously increased again at the third time point （P 〈 0.05）. Compared with EP group, MDA content of E group was no obviously differ- ent, while MDA content of E group was higher. （2）Rat cerebral cortex micro - structure and ultra- structure ： The injuring of neuron nucleus and mitochondrion those in EP group were all smaller than in E group at each time point, which indicated that high -intensity interval training can be effective in preventing a result of a one - time exhausted the body＇s ability to get rid of free radicals. cerebral ischemia- reperfusion injury and enhance