CAJ | 학술논문

应用放射免疫分析法（CharmⅡ）检测动物组织及尿样中残留四环素类药物，对猪的组织及尿样进行了金霉素标准品添加验证试验．选取6个样品进行添加并时每个样品检测3次，结果显示猪尿样在加标点1000μg／L，稀释因子50的条件下，其检测值在同一样品中变异系数0．50％～2．63％，样品间变异系数5．05％；猪的组织（猪瘦肉）在加标点100μg／kg，稀释因子4的条件下，其检测值在同一样品中变异系数0．49％～7．44％，样品间变异系数18．33％．对猪尿样降低稀释因子由说明书要求的150降到50，结果表明在稀释因子50的条件下，增大了的样本背景干扰对试剂盒检测基本无影响．应用该方法进行四环素类药物残留的大类筛选，其结果检测稳定性良好．
응용방사면역분석법（CharmⅡ）검측동물조직급뇨양중잔류사배소류약물，대저적조직급뇨양진행료금매소표준품첨가험증시험．선취6개양품진행첨가병시매개양품검측3차，결과현시저뇨양재가표점1000μg／L，희석인자50적조건하，기검측치재동일양품중변이계수0．50％～2．63％，양품간변이계수5．05％；저적조직（저수육）재가표점100μg／kg，희석인자4적조건하，기검측치재동일양품중변이계수0．49％～7．44％，양품간변이계수18．33％．대저뇨양강저희석인자유설명서요구적150강도50，결과표명재희석인자50적조건하，증대료적양본배경간우대시제합검측기본무영향．응용해방법진행사배소류약물잔류적대류사선，기결과검측은정성량호．
Charm Ⅱ radioimmunoassay was established for the total-tetracyclines residues in pork and urine. We have made verification tests to add the staiadard solution of chlorotetracycline in negative residues samples of pork and urine, selected 6 samples of pork and urine, respectively, and detected 3 times for each sample. For added samples of urine, the coefficient of variation （CV） of intra-group is between 0.50% to 2.63%, the CV of inter-group is 5.05 % at punctuate 1 000μg/L and dilution factor as 50 For added samples of pork, the CV of intra-group is between 0.49% to 7.44 %, the CV of inter-group is 18. 33 % at punctuate 100 μg/kg and dilution factor as 4. Its result suggests that therers almost no affection for the lower dilution factor from 150 to 50 which increases the background interference. The results of confirmatory tests suggest that the radioimmunoassay method can be used for the screening of tetracyclines residues in pork and urine.