CAJ | 학술논문

观察有氧运动联合谷氨酰胺（Gln）补充对2型糖尿病（T2DM）大鼠血清胰高血糖素样肽-1（GLP-1）、胰岛素（INS）及空腹血糖（FBG）水平的影响。将雄性SD大鼠60只（（179．8±19．2）g）随机分为健康对照组（C组，26只）和糖尿病造模组（D组，34只）。C组普通饲料喂养，D组高脂喂养。4周后D组大鼠腹腔注射35mg／kg链脲佐菌素（STZ）诱导T2DM。成模后两组大鼠进一步随机分为：安静组（CQ、DQ）、运动组（CE、DE）、Gln组（CG、DG）、运动加Gln组（CEG、DEG）。运动组大鼠进行6周游泳运动。Gln组改用质量分数为2％L-Gln饲料喂养。腹主动脉取血测FBG、胰岛素及GLP-1水平。结果：6周游泳运动或Gln补充，均明显提高D组大鼠GLP-1和胰岛素水平，显著降低FBG值，明显改善大鼠多饮多食症状，但对体重影响不明显。6周运动明显增加C组大鼠胰岛素水平，显著降低其FBG值，对GLP-1没有明显影响；6用的Gln补充明显降低C组大鼠的进食量，对FBG、胰岛素及GLP-1水平均无明显影响。当运动加Gln补充时，对D组大鼠FBG的控制、血浆GLP-1的增加、胰岛素水平的提高以及消耗症状的改善等均较运动或Gln补充单独作用时明显，但对体重的影响没有明显差异；二者联合对C组大鼠GLP-1的影响较运动或Gln补充单独作用时明显，但对FBG、胰岛素和体重的影响没有明显差异。结果表明：长期有氧运动或Gln补充可提高T2DM大鼠GLP-1水平，增加胰岛素分泌，降低血糖，改善多食多饮症状。当运动联合Gln补充时，降低T2DM大鼠血糖及升高GLP-1和胰岛素水平均较运动或Gln单因素作用明显。
관찰유양운동연합곡안선알（Gln）보충대2형당뇨병（T2DM）대서혈청이고혈당소양태-1（GLP-1）、이도소（INS）급공복혈당（FBG）수평적영향。장웅성SD대서60지（（179．8±19．2）g）수궤분위건강대조조（C조，26지）화당뇨병조모조（D조，34지）。C조보통사료위양，D조고지위양。4주후D조대서복강주사35mg／kg련뇨좌균소（STZ）유도T2DM。성모후량조대서진일보수궤분위：안정조（CQ、DQ）、운동조（CE、DE）、Gln조（CG、DG）、운동가Gln조（CEG、DEG）。운동조대서진행6주유영운동。Gln조개용질량분수위2％L-Gln사료위양。복주동맥취혈측FBG、이도소급GLP-1수평。결과：6주유영운동혹Gln보충，균명현제고D조대서GLP-1화이도소수평，현저강저FBG치，명현개선대서다음다식증상，단대체중영향불명현。6주운동명현증가C조대서이도소수평，현저강저기FBG치，대GLP-1몰유명현영향；6용적Gln보충명현강저C조대서적진식량，대FBG、이도소급GLP-1수평균무명현영향。당운동가Gln보충시，대D조대서FBG적공제、혈장GLP-1적증가、이도소수평적제고이급소모증상적개선등균교운동혹Gln보충단독작용시명현，단대체중적영향몰유명현차이；이자연합대C조대서GLP-1적영향교운동혹Gln보충단독작용시명현，단대FBG、이도소화체중적영향몰유명현차이。결과표명：장기유양운동혹Gln보충가제고T2DM대서GLP-1수평，증가이도소분비，강저혈당，개선다식다음증상。당운동연합Gln보충시，강저T2DM대서혈당급승고GLP-1화이도소수평균교운동혹Gln단인소작용명현。
In order to observe the effects of aerobic exercising combined with glutamine （Gln） supplement on the serum glucagon like peptide 1 （GLP-1）, insulin （INS） and fasting blood glucose （FBG） levels of rats with type 2 diabetes mellitus （T2DM）, the authors divided 60 male SD rats （（179.8＋19.2） g） randomly into a healthy control group （group C, 26 rats） and a diabetic model establishment ground （group D, 34 rats）. The rats in group C were fed with common feed, while the rats in group D were fed with high fat feed. 4 weeks later, the rats in group D were in- jected with 35 mg/kg of streptozotocin （STZ） to induce T2DM. After model formation, the rats in the two groups were further divided into calm groups （CQ, DQ）, exercising groups （CE, DE）, Gin groups （CG, DG） and exercising plus Gin groups （CEG~ DEG） randomly. The rats in the exercising groups did a 6-week swimming exercise. The rats in the Gln groups were fed with Gln a mass fraction of 2% L. Abdominal aorta blood was taken to test FBC1 insulin and GLP-1 levels. Results： 6-week swimming exercise or Gin supplement significantly increased the GLP-1 and insulin concentrations of the rats in group D, significantly decreased FBG value, significantly improved the symptom of polyphagia of the rats in group D, but had no significant effect on the body weight. 6-week exercising significantly increased the insulin level of the rats in group C, significantly decreased their FBG value, had no significant effect on GLP-1; 6-week Gin supplement significantly decreased the food intake of the rats in group C, had no significant effect on their FBG insulin and GLP-1 levels. When exercising was combined with Gln supplement, the control of FBG the increase of plasma GLP-1, the increase of insulin level and the improvement of consumption symptom of the rats in group D were more significant than those showed when exercising or Gin supplement worked separately, but there was no significant difference in the effect on the body weight; the effect of the combi- nation of exercising and Gin supplement on GLP-1 of the rats in group C was more significant than the effect produced when exercising or Gin supplement worked separately, but had no significant effect on FGB, insulin and body weight. The results indicated the followings： long term aerobic exercising or Gin supplement can increase the GLP-1 level of rats with T2DM, increase their insulin secretion, lower their blood sugar, and improve their polyphagia symptom; when exercising is combined with Gin supplement, it can lower the blood sugar of rats with T2DM, and its effect on increasing their GLP-1 and insulin levels is more significant than the effect produced when exercising or Gin supplement works as a single factor.