CAJ | 학술논문

目的：旨在研究运动对AMPKα2三种不同基因状态鼠骨骼肌细胞核内HDAC5/PGC1-MEF2结合量的影响,以探讨运动提高骨骼肌MEF2-GLUT4 DNA结合活性的可能机制。方法：野生鼠,AMPKα2基因高表达和敲除鼠各20只,分别随机分为安静对照组和跑台运动组,运动时间均为1 h,跑台速度为20 m/min。运动后3 h取材。结果：野生鼠1 h跑台运动后,骨骼肌细胞核内HDAC5与MEF2结合量显著减低,而PGC1和MEF2结合量显著升高;AMPKα2基因高表达及敲除鼠骨骼肌细胞核内HDAC5与MEF2结合量与野生鼠相比均没有显著差异,AMPKα2基因高表达鼠骨骼肌细胞核内PGC1与MEF2结合量显著高于野生鼠。结论：运动可能通过减少骨骼肌细胞核内MEF2和HDAC5结合量,并提高MEF2和PGC1的结合量而调节MEF2/GLUT4结合活性;AMPKα2参与调解运动诱导的MEF2/GLUT4的结合活性的增加并不是通过降低HDAC5和MEF2结合量实现的,而是通过提高PGC1与MEF2结合量增多而实现。
목적：지재연구운동대AMPKα2삼충불동기인상태서골격기세포핵내HDAC5/PGC1-MEF2결합량적영향,이탐토운동제고골격기MEF2-GLUT4 DNA결합활성적가능궤제。방법：야생서,AMPKα2기인고표체화고제서각20지,분별수궤분위안정대조조화포태운동조,운동시간균위1 h,포태속도위20 m/min。운동후3 h취재。결과：야생서1 h포태운동후,골격기세포핵내HDAC5여MEF2결합량현저감저,이PGC1화MEF2결합량현저승고;AMPKα2기인고표체급고제서골격기세포핵내HDAC5여MEF2결합량여야생서상비균몰유현저차이,AMPKα2기인고표체서골격기세포핵내PGC1여MEF2결합량현저고우야생서。결론：운동가능통과감소골격기세포핵내MEF2화HDAC5결합량,병제고MEF2화PGC1적결합량이조절MEF2/GLUT4결합활성;AMPKα2삼여조해운동유도적MEF2/GLUT4적결합활성적증가병불시통과강저HDAC5화MEF2결합량실현적,이시통과제고PGC1여MEF2결합량증다이실현。
Objective： The effects of exercise on the content of MEF2–bound HDAC5/PGC1 were investigated in skeletal muscle of AMPKα2-WT/KO/OE mice to explore the possible mechanism of improving the association activity of the MEF2-GLUT4 DNA of skeletal muscle.Methods： Wild-type（WT;n=20）,AMPKα2 over-expression（OE;n=20） and knockout（KO;n=20） C57/BL mice were randomly subdivided into two groups： control group and exercise group.Mice were killed 3 h after treadmill running for 60 min at 20 m/min on the experimental day.Results： After one hour running exercise,MEF2–bound HDAC5 decreased,whereas MEF2–bound PGC1 increased in wild type mice.MEF2 bound HDAC5 was normal in α2-OE and α2-KO muscles after 1 hour exercise.MEF2-combined PGC1 in OE mice was higher than that in WT mice.Conclusion： Exercise increases binding of MEF2A binding by decreasing HDAC5-MEF2 complexes and increasing PGC1-MEF2 complexes;Exercise-induced AMPK activation increases the binding of MEF2A to the Glut4 promoter by a mechanism not involving the decreased nuclear HDAC5-MEF2 complexes,but involving the increased nuclear PGC-1 combined with MEF2.