北京体育大学学报
北京體育大學學報
북경체육대학학보
Journal of Beijing University of Physical Education
2012年
10期
63~69
,共null页
龚豪杰 谢谨 张楠 张缨
龔豪傑 謝謹 張楠 張纓
공호걸 사근 장남 장영
葡萄糖运载体4 肌细胞增强因子2 组蛋白去乙酰化酶5 过氧化物酶体增生物激活受体γ共激活因子1
葡萄糖運載體4 肌細胞增彊因子2 組蛋白去乙酰化酶5 過氧化物酶體增生物激活受體γ共激活因子1
포도당운재체4 기세포증강인자2 조단백거을선화매5 과양화물매체증생물격활수체γ공격활인자1
GLUT4; MEF2; HDAC5; PGC1
目的:旨在研究运动对AMPKα2三种不同基因状态鼠骨骼肌细胞核内HDAC5/PGC1-MEF2结合量的影响,以探讨运动提高骨骼肌MEF2-GLUT4 DNA结合活性的可能机制。方法:野生鼠,AMPKα2基因高表达和敲除鼠各20只,分别随机分为安静对照组和跑台运动组,运动时间均为1 h,跑台速度为20 m/min。运动后3 h取材。结果:野生鼠1 h跑台运动后,骨骼肌细胞核内HDAC5与MEF2结合量显著减低,而PGC1和MEF2结合量显著升高;AMPKα2基因高表达及敲除鼠骨骼肌细胞核内HDAC5与MEF2结合量与野生鼠相比均没有显著差异,AMPKα2基因高表达鼠骨骼肌细胞核内PGC1与MEF2结合量显著高于野生鼠。结论:运动可能通过减少骨骼肌细胞核内MEF2和HDAC5结合量,并提高MEF2和PGC1的结合量而调节MEF2/GLUT4结合活性;AMPKα2参与调解运动诱导的MEF2/GLUT4的结合活性的增加并不是通过降低HDAC5和MEF2结合量实现的,而是通过提高PGC1与MEF2结合量增多而实现。
目的:旨在研究運動對AMPKα2三種不同基因狀態鼠骨骼肌細胞覈內HDAC5/PGC1-MEF2結閤量的影響,以探討運動提高骨骼肌MEF2-GLUT4 DNA結閤活性的可能機製。方法:野生鼠,AMPKα2基因高錶達和敲除鼠各20隻,分彆隨機分為安靜對照組和跑檯運動組,運動時間均為1 h,跑檯速度為20 m/min。運動後3 h取材。結果:野生鼠1 h跑檯運動後,骨骼肌細胞覈內HDAC5與MEF2結閤量顯著減低,而PGC1和MEF2結閤量顯著升高;AMPKα2基因高錶達及敲除鼠骨骼肌細胞覈內HDAC5與MEF2結閤量與野生鼠相比均沒有顯著差異,AMPKα2基因高錶達鼠骨骼肌細胞覈內PGC1與MEF2結閤量顯著高于野生鼠。結論:運動可能通過減少骨骼肌細胞覈內MEF2和HDAC5結閤量,併提高MEF2和PGC1的結閤量而調節MEF2/GLUT4結閤活性;AMPKα2參與調解運動誘導的MEF2/GLUT4的結閤活性的增加併不是通過降低HDAC5和MEF2結閤量實現的,而是通過提高PGC1與MEF2結閤量增多而實現。
목적:지재연구운동대AMPKα2삼충불동기인상태서골격기세포핵내HDAC5/PGC1-MEF2결합량적영향,이탐토운동제고골격기MEF2-GLUT4 DNA결합활성적가능궤제。방법:야생서,AMPKα2기인고표체화고제서각20지,분별수궤분위안정대조조화포태운동조,운동시간균위1 h,포태속도위20 m/min。운동후3 h취재。결과:야생서1 h포태운동후,골격기세포핵내HDAC5여MEF2결합량현저감저,이PGC1화MEF2결합량현저승고;AMPKα2기인고표체급고제서골격기세포핵내HDAC5여MEF2결합량여야생서상비균몰유현저차이,AMPKα2기인고표체서골격기세포핵내PGC1여MEF2결합량현저고우야생서。결론:운동가능통과감소골격기세포핵내MEF2화HDAC5결합량,병제고MEF2화PGC1적결합량이조절MEF2/GLUT4결합활성;AMPKα2삼여조해운동유도적MEF2/GLUT4적결합활성적증가병불시통과강저HDAC5화MEF2결합량실현적,이시통과제고PGC1여MEF2결합량증다이실현。
Objective: The effects of exercise on the content of MEF2–bound HDAC5/PGC1 were investigated in skeletal muscle of AMPKα2-WT/KO/OE mice to explore the possible mechanism of improving the association activity of the MEF2-GLUT4 DNA of skeletal muscle.Methods: Wild-type(WT;n=20),AMPKα2 over-expression(OE;n=20) and knockout(KO;n=20) C57/BL mice were randomly subdivided into two groups: control group and exercise group.Mice were killed 3 h after treadmill running for 60 min at 20 m/min on the experimental day.Results: After one hour running exercise,MEF2–bound HDAC5 decreased,whereas MEF2–bound PGC1 increased in wild type mice.MEF2 bound HDAC5 was normal in α2-OE and α2-KO muscles after 1 hour exercise.MEF2-combined PGC1 in OE mice was higher than that in WT mice.Conclusion: Exercise increases binding of MEF2A binding by decreasing HDAC5-MEF2 complexes and increasing PGC1-MEF2 complexes;Exercise-induced AMPK activation increases the binding of MEF2A to the Glut4 promoter by a mechanism not involving the decreased nuclear HDAC5-MEF2 complexes,but involving the increased nuclear PGC-1 combined with MEF2.