山东体育学院学报
山東體育學院學報
산동체육학원학보
Journal of Shandong Institute Of Physical Education And Sports
2012年
6期
68~72
,共null页
烟酸 耐力训练 急性酒精性肝损伤 沉默信息调节因子
煙痠 耐力訓練 急性酒精性肝損傷 沉默信息調節因子
연산 내력훈련 급성주정성간손상 침묵신식조절인자
niacin; endurance training; alcohol -induced acute hepatic injury; silent information regulator
目的：研究补充烟酸及耐力训练对急性酒精性肝损伤大鼠SifT1表达、活性的影响及机制。研究方法：以Wistar大鼠建立急性酒精性肝损伤模型，以跑台运动为训练手段，并且给予大鼠补充烟酸，测定血清AIJT和As以及肝脏MnSOD活性、NAD＋／NADH比值、MDA含量、sirT1活性、线粒体ATP合成活力、MnSOD和sirT1mRNA表达。结果：急性酒精摄入导致血清ALT和AST水平、肝脏MDA含量均显著升高，线粒体ATP合成活力、肝脏NAD’／NADH比值、MnSOD及SirTl活性及mRNA表达均显著降低；耐力训练及补充烟酸再给予酒精摄入与只给予酒精摄入血清ALT和AST水平、肝脏MDA含量、线粒体ArrP合成活力、肝脏NAD＋／NADH比值、MnSOD及SirT1活性及mRNA表达变化规律一致，但变化幅度相对较小。结论：耐力训练及补充烟酸均能提高肝脏细胞内NAD＋／NADH的比值，使SirT1活性及表达升高，进而提高肝脏细胞抗氧化能力，达到预防／改善急性酒精性肝损伤的目的。
目的：研究補充煙痠及耐力訓練對急性酒精性肝損傷大鼠SifT1錶達、活性的影響及機製。研究方法：以Wistar大鼠建立急性酒精性肝損傷模型，以跑檯運動為訓練手段，併且給予大鼠補充煙痠，測定血清AIJT和As以及肝髒MnSOD活性、NAD＋／NADH比值、MDA含量、sirT1活性、線粒體ATP閤成活力、MnSOD和sirT1mRNA錶達。結果：急性酒精攝入導緻血清ALT和AST水平、肝髒MDA含量均顯著升高，線粒體ATP閤成活力、肝髒NAD’／NADH比值、MnSOD及SirTl活性及mRNA錶達均顯著降低；耐力訓練及補充煙痠再給予酒精攝入與隻給予酒精攝入血清ALT和AST水平、肝髒MDA含量、線粒體ArrP閤成活力、肝髒NAD＋／NADH比值、MnSOD及SirT1活性及mRNA錶達變化規律一緻，但變化幅度相對較小。結論：耐力訓練及補充煙痠均能提高肝髒細胞內NAD＋／NADH的比值，使SirT1活性及錶達升高，進而提高肝髒細胞抗氧化能力，達到預防／改善急性酒精性肝損傷的目的。
목적：연구보충연산급내력훈련대급성주정성간손상대서SifT1표체、활성적영향급궤제。연구방법：이Wistar대서건립급성주정성간손상모형，이포태운동위훈련수단，병차급여대서보충연산，측정혈청AIJT화As이급간장MnSOD활성、NAD＋／NADH비치、MDA함량、sirT1활성、선립체ATP합성활력、MnSOD화sirT1mRNA표체。결과：급성주정섭입도치혈청ALT화AST수평、간장MDA함량균현저승고，선립체ATP합성활력、간장NAD’／NADH비치、MnSOD급SirTl활성급mRNA표체균현저강저；내력훈련급보충연산재급여주정섭입여지급여주정섭입혈청ALT화AST수평、간장MDA함량、선립체ArrP합성활력、간장NAD＋／NADH비치、MnSOD급SirT1활성급mRNA표체변화규률일치，단변화폭도상대교소。결론：내력훈련급보충연산균능제고간장세포내NAD＋／NADH적비치，사SirT1활성급표체승고，진이제고간장세포항양화능력，체도예방／개선급성주정성간손상적목적。
Objective：To investigate the influence of niacin supplement and endurance training on the activity and expression of SirT1 and its mechanism in alcohol - induced acute hepatic injury in rats. Methods：It analyzed the level of ALT and AST in serum, NAD ＋/NADH, content of MDA, mito- chondrial ATP synthesis, activities and expressions of MnSOD and SirT1 in liver tissue in alcohol - induced acute hepatic injury rats after treadmill training and niacin supplement. Results： The acute alcohol treatment increases ALT and AST in serum and content of MDA in liver tissue, decreases mitochondrial ATP synthesis, ratio of NAD ~/NADH, activities and expression of MnSOD and SirT1 in alcohol - induced acute hepatic injury rats. The parameters of the above indicates the same after endurance training and niacin supplement but the extent is relatively lower. Conclusion：Endurance training and niacin supplement can increase ration of NAD ＋/NADH, which improves the activity and expression of SirT1, and then increases antioxidant ability of liver, which achieves the goal of protecting or improving alcoholinduced acute hepatic injury.
