北京体育大学学报
北京體育大學學報
북경체육대학학보
Journal of Beijing University of Physical Education
2013年
1期
64~68
,共null页
中等强度运动 左室肌 蛋白质组 差异表达 运动心脏重塑 能量代谢蛋白 热休克蛋白
中等彊度運動 左室肌 蛋白質組 差異錶達 運動心髒重塑 能量代謝蛋白 熱休剋蛋白
중등강도운동 좌실기 단백질조 차이표체 운동심장중소 능량대사단백 열휴극단백
moderate intensity exercise ; the muscle of left ventricular; protein group ; the differentia expression;exercise heart remodeling; energy metabolism protein; heart shock protein
目的：采用蛋白质组学技术，探究中等强度运动后大鼠左室肌蛋白质组的差异表达。方法：将SD大鼠分为运动组和对照组，训练完成后麻醉处死，计量大鼠心重，提取左室肌全蛋白样品，采用2一DE进行分离，选择运动后差异表达量≥10倍的备选目标蛋白点，进行质谱鉴定。结果：取5个目标蛋白点，经质谱鉴定4个。未知蛋白质训练后表达量下调≥12．5倍。结论：1）中等强度运动后，大鼠心脏发生形态学变化使心肌增厚，心收缩力增强；2）训练组大鼠在长期运动训练中，左心室心肌蛋白质组变化显著；3）鉴定出运动医学中还未涉及的4种蛋白质，分别是三磷酸腺苷合酶偶合因子6（CF6）、葡萄糖调节蛋白78（GRP78）、烯酰辅酶A水合酶（ECH）和ATP合酶a亚基（ATP synthase subunit alpha），其表达发生的顺应性改变说明，中等强度运动可提高心肌的能量代谢水平。
目的：採用蛋白質組學技術，探究中等彊度運動後大鼠左室肌蛋白質組的差異錶達。方法：將SD大鼠分為運動組和對照組，訓練完成後痳醉處死，計量大鼠心重，提取左室肌全蛋白樣品，採用2一DE進行分離，選擇運動後差異錶達量≥10倍的備選目標蛋白點，進行質譜鑒定。結果：取5箇目標蛋白點，經質譜鑒定4箇。未知蛋白質訓練後錶達量下調≥12．5倍。結論：1）中等彊度運動後，大鼠心髒髮生形態學變化使心肌增厚，心收縮力增彊；2）訓練組大鼠在長期運動訓練中，左心室心肌蛋白質組變化顯著；3）鑒定齣運動醫學中還未涉及的4種蛋白質，分彆是三燐痠腺苷閤酶偶閤因子6（CF6）、葡萄糖調節蛋白78（GRP78）、烯酰輔酶A水閤酶（ECH）和ATP閤酶a亞基（ATP synthase subunit alpha），其錶達髮生的順應性改變說明，中等彊度運動可提高心肌的能量代謝水平。
목적：채용단백질조학기술，탐구중등강도운동후대서좌실기단백질조적차이표체。방법：장SD대서분위운동조화대조조，훈련완성후마취처사，계량대서심중，제취좌실기전단백양품，채용2일DE진행분리，선택운동후차이표체량≥10배적비선목표단백점，진행질보감정。결과：취5개목표단백점，경질보감정4개。미지단백질훈련후표체량하조≥12．5배。결론：1）중등강도운동후，대서심장발생형태학변화사심기증후，심수축력증강；2）훈련조대서재장기운동훈련중，좌심실심기단백질조변화현저；3）감정출운동의학중환미섭급적4충단백질，분별시삼린산선감합매우합인자6（CF6）、포도당조절단백78（GRP78）、희선보매A수합매（ECH）화ATP합매a아기（ATP synthase subunit alpha），기표체발생적순응성개변설명，중등강도운동가제고심기적능량대사수평。
Objective： Using the technology of proteomics, the study probed into the differentially expressive pro- teome of the left ventricular muscle after medium intensity training. Methods ： The researchers divided SD rats into control group and exercise group. After exercise, the rats were killed after anesthesia. The researchers weighed heart of each rat, extracted whole protein samples of the left ventrieular muscle, and separated the protein samples by utilizing two-dimensional gel electrophoresis. The ahernatively objective protein spots whose differential expres- sive volume over lO-fold were selected for MS identification. Resuhs： Taking out five alternatively objective pro- tein spots, the researchers identified four by mass spectrum. The expression volume decreased more than 12.5- folds after unknown protein training. Conclusion ： 1 ） The morphologic changes of the rat heart made the cardiac muscle inerassation and strengthened the heart contraction after 8 weeks＇ medium intensity exercise. 2 ） During long-time training, the rats in the exercise group had significant change in the proteome of the left ventricular mus- cle. 3 ）The researchers identified 4 proteins which has not referred in the sports medicine field at present, name- ly, ATP synthase coupling factor 6, glucose-regulated protein 78, Enoyl-CoA hydratase and ATP synthase subunit alpha. The compliance changes indicated that moderate intensity training can improve the energy metabolism level of the cardiac muscle.
