CAJ | 학술논문

目的：研究短期力竭运动及其恢复期间大鼠心肌超微结构及心肌营养素-1的变化。方法：8周龄健康雄性大鼠36只为研究对象,将其随即分为安静对照组（C）、力竭运动后即刻（Exhaustive,E0）、后12 h组（E12）、后24 h组（E24）、后48 h组（E48）、后72 h（E72）6组,每组6只。各运动组分别进行为期一周的力竭运动,安静对照组和力竭运动组分别于安静状态和末次力竭运动后不同时间点宰杀,迅速采取心肌样本,电镜观察大鼠心肌超微结构的变化,采用ELISA酶联免疫吸附反应法检测大鼠心肌营养素-1。结果：力竭运动后及恢复期心肌超微形态结构发生了不同程度的改变,其中以E0组和E24组较明显,心肌肌原纤维紊乱、扭曲,局部线粒体数量减少,甚至出现膨胀和变形。另一方面,大鼠在进行一周的大负荷运动后,心肌中的心肌营养素-1含量发生变化,力竭运动组（E0、E12、E24、E48、E72）与安静对照组相比都有显著性升高（P〈0.01）;其中,心肌营养素-1在E0组表达最显著,E72组有所回落（P〈0.01）。结论：大负荷运动后及恢复期间,大鼠心肌内部结构遭到一定程度的破坏,同时大鼠心肌中的心肌营养素-1含量明显提高,且在E0组表达最高,提示大鼠心肌营养素-1在心肌损伤的修复中可能发挥重要作用。
목적：연구단기력갈운동급기회복기간대서심기초미결구급심기영양소-1적변화。방법：8주령건강웅성대서36지위연구대상,장기수즉분위안정대조조（C）、력갈운동후즉각（Exhaustive,E0）、후12 h조（E12）、후24 h조（E24）、후48 h조（E48）、후72 h（E72）6조,매조6지。각운동조분별진행위기일주적력갈운동,안정대조조화력갈운동조분별우안정상태화말차력갈운동후불동시간점재살,신속채취심기양본,전경관찰대서심기초미결구적변화,채용ELISA매련면역흡부반응법검측대서심기영양소-1。결과：력갈운동후급회복기심기초미형태결구발생료불동정도적개변,기중이E0조화E24조교명현,심기기원섬유문란、뉴곡,국부선립체수량감소,심지출현팽창화변형。령일방면,대서재진행일주적대부하운동후,심기중적심기영양소-1함량발생변화,력갈운동조（E0、E12、E24、E48、E72）여안정대조조상비도유현저성승고（P〈0.01）;기중,심기영양소-1재E0조표체최현저,E72조유소회락（P〈0.01）。결론：대부하운동후급회복기간,대서심기내부결구조도일정정도적파배,동시대서심기중적심기영양소-1함량명현제고,차재E0조표체최고,제시대서심기영양소-1재심기손상적수복중가능발휘중요작용。
Objective： The purpose of this paper was to study the changes of cardiac muscle uhrastrueture and to ex- plore the changes of cardiotrophin - 1 during heavy load exercise and recovery in rat. Methods ： 36 male SD rats of 8 weeks old were randomly divided in 6 groups, and each group had six rats. Control group （ C ） and after exhaus- tive exercise group （Exhaustive,E0）, 12 h group （El2）, 24 h group （E24）, 48 h group （FA8）, 72 h group （E72）. The rats of exercise groups carried on exhaustive loaded swimming every day for one week. After the last exhaustive exercise at different time points, each rat was anesthetized intraperitoneally to slaughter, and cardiac muscles were taken. A part of cardiac muscle were fixed for producing ultrathin sections and observed to the ultra- structural changes by transmission electron microscope. The other part was to measure cardiotrophin - 1 by Elisa. Result ： After exhaustive exercise, cardiac muscle ultrastruetures had different changes in E0 and E24. The myofi- bril disorder and distorted in a range. Some mitochondrias were reduced, swollen and deformed. On the other hand, the activity of CT- 1 in all exhaustive exercise groups （E0, El2, E24, E48, E72） had a significant im- provement compared with the control group（P 〈 0.01 ）. Especially, the cardiotrophin -1 （CT- 1 ） of the cardiac muscle clearly expressed to a peak in E0, then reduced significantly in E72 （P 〈 0.01 ）. Conclusion： The ultra- structure of cardiac muscle structure in exhaustive exercise groups were significantly damaged during heavy load exercise and recovery. At same time, the eardiotrophin - 1 （ CT - 1 ） of cardiac muscle was increased during heavy load exercise and recovery. CT - 1 expressed to a peak at E0,and it indicated that cardiotrophin - 1 can be an important role to accelerate the repair of cardiac muscle injury.