周口师范学院学报
週口師範學院學報
주구사범학원학보
Journal of Zhoukou Normal University
2013年
2期
56~58
,共null页
抗EGFR单克隆抗体 体积排阻色谱法 离子交换色谱法 非还原SDS—PAGE 电荷异构体
抗EGFR單剋隆抗體 體積排阻色譜法 離子交換色譜法 非還原SDS—PAGE 電荷異構體
항EGFR단극륭항체 체적배조색보법 리자교환색보법 비환원SDS—PAGE 전하이구체
anti - EGFR monoclonal antibody; size exclusion chromatography; ion exchange chromatography; non - re-ducing SDS - PAGE; charge isoforms
应用体积排阻色谱法、非还原SDS—PAGE法、离子交换色谱法分析抗EGFR单克隆抗体纯度．结果表明：三种分析方法分析抗EGFR单克隆抗体纯度表现出明显差异，体积排阻色谱法检测出多聚体含量、非还原SDS—PAGE法检测出降解片段的含量、离子交换色谱法检测出多个电荷异构体．基于单克隆抗体结构的多样性，有必要开发多种分析方法监控单克隆抗体的纯度．
應用體積排阻色譜法、非還原SDS—PAGE法、離子交換色譜法分析抗EGFR單剋隆抗體純度．結果錶明：三種分析方法分析抗EGFR單剋隆抗體純度錶現齣明顯差異，體積排阻色譜法檢測齣多聚體含量、非還原SDS—PAGE法檢測齣降解片段的含量、離子交換色譜法檢測齣多箇電荷異構體．基于單剋隆抗體結構的多樣性，有必要開髮多種分析方法鑑控單剋隆抗體的純度．
응용체적배조색보법、비환원SDS—PAGE법、리자교환색보법분석항EGFR단극륭항체순도．결과표명：삼충분석방법분석항EGFR단극륭항체순도표현출명현차이，체적배조색보법검측출다취체함량、비환원SDS—PAGE법검측출강해편단적함량、리자교환색보법검측출다개전하이구체．기우단극륭항체결구적다양성，유필요개발다충분석방법감공단극륭항체적순도．
The purity of anti- EGFR monoclonal antibody was analyzed by such analysis methods as size exclusion chro- matography（SEC）, non- reducing SDS -PAGE and ion exchange chromatography. Significant difference was observed in pu- rity analysis, which was showed that aggregates could be detected by SEC, degradation fragment was found by non- reduc- ing SDS- PAGE, and several charge isoforms were detected by ion exchange chromatography. It is essential to apply many analysis methods to the purity of monoclonal antibody due to its structure diversity.
