CAJ | 학술논문

摘要：以河南宋河白酒发酵30d的糟醅为实验材料，通过富集培养及稀释涂布法分离厌氧及兼性厌氧菌，以菌落计数法确定最佳样品稀释度和适宜的厌氧菌培养方法，并采用形态学和生理生化特性进行种属的初步鉴定．结果表明：厌氧及兼性厌氧菌分离适宜稀释度为10^-3，最佳培养方法为抽真空培养法，共分离到5株己酸菌、6株丁酸菌、2株甲烷菌、6株乳酸菌．丁酸菌、己酸菌和乳酸菌革兰氏染色均为阳性，甲烷菌为革兰氏阴性菌．丁酸菌和己酸菌分别归梭菌属（Clostridium）和芽孢杆菌属（Bacillus），甲烷菌为假单胞菌属（Pseudomonas），乳酸菌为芽孢乳杆菌属（Sporolactobacillus）.
적요：이하남송하백주발효30d적조배위실험재료，통과부집배양급희석도포법분리염양급겸성염양균，이균락계수법학정최가양품희석도화괄의적염양균배양방법，병채용형태학화생리생화특성진행충속적초보감정．결과표명：염양급겸성염양균분리괄의희석도위10^-3，최가배양방법위추진공배양법，공분리도5주기산균、6주정산균、2주갑완균、6주유산균．정산균、기산균화유산균혁란씨염색균위양성，갑완균위혁란씨음성균．정산균화기산균분별귀사균속（Clostridium）화아포간균속（Bacillus），갑완균위가단포균속（Pseudomonas），유산균위아포유간균속（Sporolactobacillus）.
Anaerobic and facultative anaerobic bacteria were identified and isolated from songhe grain fermented for 30 days, strains were isolated by enrichment culturing and dilution coating plate, The best dilution and anaerobic cultured meth- od was determined by counting colony, as well, strains isolated were identified with the method of observing physical charac- terization and testing biochemical properties. The results showed that the optimal dilution used to separate anaerobic and fac- ultative anaerobic bacteria was 10-3 , the cultured method of pumping vacuum was suitable for culturing and isolating anaero bic bacteria, 5 caproic acid bacteria and 6 butyric acid bacteria which gram stain were positive were obtained and identified as Clostridium and Bacillus, 2 methane bacteria with negative gram staining were isolated and identified as Pseudomonas, 6 lactic acid bacteria which gram stain were positive were obtained and identified as Sporolactobacillus