体育科学
體育科學
체육과학
China Sport Science
2013年
5期
41~49
,共null页
葛新发 董贵俊 王玉站 李可峰 潘卫东
葛新髮 董貴俊 王玉站 李可峰 潘衛東
갈신발 동귀준 왕옥참 리가봉 반위동
重复运动 骨骼肌 离心运动 蛋白质组 鼠 动物实验
重複運動 骨骼肌 離心運動 蛋白質組 鼠 動物實驗
중복운동 골격기 리심운동 단백질조 서 동물실험
repetitive exercise, skeletal muscle, eccentric exercise, proteome ; rat ; animal experitment
目的:通过蛋白质组学研究手段分析重复大强度运动对骨骼肌损伤修复过程中蛋白质组表达情况,并从蛋白质组变异角度分析重复运动对骨骼肌损伤修复可能的作用机制;方法:72只Wistar大鼠随机分为正常对照组、一次离心运动组及一周后重复运动组。对照组不运动,其余组大鼠进行下坡跑,速度为18m/min,坡度-16°,运动时间为30min后休息5min,再运动30min。运动时间共60min。重复运动在一次运动后一周后进行。在运动后即刻、24h、48h、72h、168h取股四头肌进行蛋白质提取、双向电泳及特异蛋白质鉴定,并按照蛋白质功能对特异蛋白质进行分类;结果:重复运动后的修复过程中,能量代谢相关蛋白和细胞损伤修复相关蛋白表达在重复运动组中主要表现为下调为主,特别是在运动后0~72h之间。重复运动组中骨骼肌细胞结构蛋白0~24h内上调和下调数目无明显差异,在72h时,下调数目明显多于上调蛋白数目。损伤修复过程中表达变化的蛋白质中,能量代谢相关蛋白有异柠檬酸脱氢酶、磷酸葡萄糖变位酶、烟酰胺腺嘌呤二核苷酸脱氢酶、血色素结合蛋白、丙酮酸脱氢酶、磷酸丙糖异构酶,细胞损伤修复相关蛋白有泛素羧基末端水解酶、免疫球蛋白入轻链、胞内氯离子通道蛋白、蛋白磷酸酶2C蛋白、谷胱苷肽过氧化物酶、双功能过氧化物酶等表达活跃,骨骼肌细胞结构蛋白有热休克蛋白、内质网驻地蛋白、角蛋白、肌球蛋白、线粒体内膜蛋白等。结论:重复运动后能量代谢关键酶表达,加速损伤细胞能量供应,同时在24h-48h之间进行可以减缓骨骼肌收缩蛋白降解、清除自由基、加快细胞胞吞、减缓炎症反应从而加快骨骼肌损伤修复。
目的:通過蛋白質組學研究手段分析重複大彊度運動對骨骼肌損傷脩複過程中蛋白質組錶達情況,併從蛋白質組變異角度分析重複運動對骨骼肌損傷脩複可能的作用機製;方法:72隻Wistar大鼠隨機分為正常對照組、一次離心運動組及一週後重複運動組。對照組不運動,其餘組大鼠進行下坡跑,速度為18m/min,坡度-16°,運動時間為30min後休息5min,再運動30min。運動時間共60min。重複運動在一次運動後一週後進行。在運動後即刻、24h、48h、72h、168h取股四頭肌進行蛋白質提取、雙嚮電泳及特異蛋白質鑒定,併按照蛋白質功能對特異蛋白質進行分類;結果:重複運動後的脩複過程中,能量代謝相關蛋白和細胞損傷脩複相關蛋白錶達在重複運動組中主要錶現為下調為主,特彆是在運動後0~72h之間。重複運動組中骨骼肌細胞結構蛋白0~24h內上調和下調數目無明顯差異,在72h時,下調數目明顯多于上調蛋白數目。損傷脩複過程中錶達變化的蛋白質中,能量代謝相關蛋白有異檸檬痠脫氫酶、燐痠葡萄糖變位酶、煙酰胺腺嘌呤二覈苷痠脫氫酶、血色素結閤蛋白、丙酮痠脫氫酶、燐痠丙糖異構酶,細胞損傷脩複相關蛋白有汎素羧基末耑水解酶、免疫毬蛋白入輕鏈、胞內氯離子通道蛋白、蛋白燐痠酶2C蛋白、穀胱苷肽過氧化物酶、雙功能過氧化物酶等錶達活躍,骨骼肌細胞結構蛋白有熱休剋蛋白、內質網駐地蛋白、角蛋白、肌毬蛋白、線粒體內膜蛋白等。結論:重複運動後能量代謝關鍵酶錶達,加速損傷細胞能量供應,同時在24h-48h之間進行可以減緩骨骼肌收縮蛋白降解、清除自由基、加快細胞胞吞、減緩炎癥反應從而加快骨骼肌損傷脩複。
목적:통과단백질조학연구수단분석중복대강도운동대골격기손상수복과정중단백질조표체정황,병종단백질조변이각도분석중복운동대골격기손상수복가능적작용궤제;방법:72지Wistar대서수궤분위정상대조조、일차리심운동조급일주후중복운동조。대조조불운동,기여조대서진행하파포,속도위18m/min,파도-16°,운동시간위30min후휴식5min,재운동30min。운동시간공60min。중복운동재일차운동후일주후진행。재운동후즉각、24h、48h、72h、168h취고사두기진행단백질제취、쌍향전영급특이단백질감정,병안조단백질공능대특이단백질진행분류;결과:중복운동후적수복과정중,능량대사상관단백화세포손상수복상관단백표체재중복운동조중주요표현위하조위주,특별시재운동후0~72h지간。중복운동조중골격기세포결구단백0~24h내상조화하조수목무명현차이,재72h시,하조수목명현다우상조단백수목。손상수복과정중표체변화적단백질중,능량대사상관단백유이저몽산탈경매、린산포도당변위매、연선알선표령이핵감산탈경매、혈색소결합단백、병동산탈경매、린산병당이구매,세포손상수복상관단백유범소최기말단수해매、면역구단백입경련、포내록리자통도단백、단백린산매2C단백、곡광감태과양화물매、쌍공능과양화물매등표체활약,골격기세포결구단백유열휴극단백、내질망주지단백、각단백、기구단백、선립체내막단백등。결론:중복운동후능량대사관건매표체,가속손상세포능량공응,동시재24h-48h지간진행가이감완골격기수축단백강해、청제자유기、가쾌세포포탄、감완염증반응종이가쾌골격기손상수복。
Objective:To research proteome variety on skeletal muscle damage repair process in- duced by one and repeated eccentric exercise and proteomics mechanism of skeletal muscle repairing made by repeated eccentric exercise; Methods: 72 Wistar rats were randomly divided into normal control group, one eccentric exercise group and repeat exercise group a week later. Control group was not done exercise, the rest groups were exercised with 18m/min of speed and -16 degrees of slope. Rats moved for 30min, rest for 5min and then moved for 30min. Repetitive motion was done after a week. Proteome extraction and dielectrophoresis were taken after 0h,24h,48h, 72h, 168h of one and repeated eccentric exercise, specific proteins were identified by MALDI--TOF MS and sorted according to its function. Results:Proteins expres- sion about energy metabolism and cell damage were reduced especially from Oh to 72h after repeated motion. The proteins number of decreased and increased expression were not different obviously from Oh to 24h and the decreased proteins number was more than number of in- creased proteins obviously at 72h after repeated motion. During process of damage repair, energy metabolism related proteins included isocitrate dehydrogenase, phosphate glucose mutase, nicotinamide adenine dinucleotide dehydrogenase, hemoglobin binding protein, pyruvate dehydrogenase, triose phosphate isomerase; cell damage repair related proteins were consisted of ubiquitin carboxyl terminal hydrolytic enzyme, immune globulin lambda light chain, intracellular chlorine ion channel protein, protein phosphatase 2c protein, glutathione peroxidase, double function peroxidase and express active; skeletal muscle cell structure protein have heat shock protein, endoplasmic retieulum stations protein and keratin, myosin, mitochondrial membrane protein, etc. Conclusion : Repeated eccentric exercise may promote key enzyme expression of energy metabolism and energy supply for damage cell, accelerate skeletal muscle contraction protein degradation and cell swallow, and scavenge free radicals and slow inflammatory reaction during 24-48h to speed up the skeletal muscle damage repair.