CAJ | 학술논문

目的：研究耐力训练和适量限食对骨骼肌内质网（endoplasmicreticulum，ER）分子伴侣表达及糖代谢改善的影响。方法：37只SD大鼠分为安静组（S）、限食安静组（DS）、训练组（T）和限食训练组（DT），训练大鼠采用递增负荷游泳耐力训练，限食大鼠进食量为正常进食组的70％，实验期4周。采用RT—PCR和westernblotting方法分别检测骨骼肌ER应激蛋白GRP78及ORP150的mRNA和蛋白表达，并测定血糖（bloodglucose，BG）、空腹血清胰岛素（fastingserllminsulin，FINS）、血浆胰高血糖素（glucagon，GLU）和股四头肌肌糖原（glycogen，Gn）、己糖激酶（hexokinase，HK）、丙酮酸激酶（pyruvatekinase，PK）活性。结果：（1）训练和限食及训练＋限食轻度上调GRP78或ORP150mRNA（＋24％-43％，P〉0．05）及蛋白（15％-30％，P〉0．05）表达；（2）训练和限食显著降低体重和体脂，训练＋限食作用更明显；限食明显降低安静BG，训练＋限食显著减慢BG恢复速度；训练和限食显著降低FINS水平，训练＋限食使其降低幅度更大；限食明显增加胰岛素敏感性（insulin sensitivity index，ISI）；训练、训练＋限食明显增加HK活性，训练和限食略增加、训练＋限食明显提高Gn含量；GRP78和ORP150mRNA表达与Gn含量显著正相关，ORP150蛋白表达与ISI显著正相关，Gn与HK显著正相关。结论：4周递增负荷游泳耐力训练和适量限食轻度上调了骨骼肌GRP78／ORP150表达，BG降低及其造成的肌细胞内游离糖水平低是可能的诱因；耐力训练和适量限食改善骨骼肌糖代谢，耐力训练与适量限食相结合的效果更好。
목적：연구내력훈련화괄량한식대골격기내질망（endoplasmicreticulum，ER）분자반려표체급당대사개선적영향。방법：37지SD대서분위안정조（S）、한식안정조（DS）、훈련조（T）화한식훈련조（DT），훈련대서채용체증부하유영내력훈련，한식대서진식량위정상진식조적70％，실험기4주。채용RT—PCR화westernblotting방법분별검측골격기ER응격단백GRP78급ORP150적mRNA화단백표체，병측정혈당（bloodglucose，BG）、공복혈청이도소（fastingserllminsulin，FINS）、혈장이고혈당소（glucagon，GLU）화고사두기기당원（glycogen，Gn）、기당격매（hexokinase，HK）、병동산격매（pyruvatekinase，PK）활성。결과：（1）훈련화한식급훈련＋한식경도상조GRP78혹ORP150mRNA（＋24％-43％，P〉0．05）급단백（15％-30％，P〉0．05）표체；（2）훈련화한식현저강저체중화체지，훈련＋한식작용경명현；한식명현강저안정BG，훈련＋한식현저감만BG회복속도；훈련화한식현저강저FINS수평，훈련＋한식사기강저폭도경대；한식명현증가이도소민감성（insulin sensitivity index，ISI）；훈련、훈련＋한식명현증가HK활성，훈련화한식략증가、훈련＋한식명현제고Gn함량；GRP78화ORP150mRNA표체여Gn함량현저정상관，ORP150단백표체여ISI현저정상관，Gn여HK현저정상관。결론：4주체증부하유영내력훈련화괄량한식경도상조료골격기GRP78／ORP150표체，BG강저급기조성적기세포내유리당수평저시가능적유인；내력훈련화괄량한식개선골격기당대사，내력훈련여괄량한식상결합적효과경호。
Purpose： The purpose is to study the effect of endurance training and moderate caloric restriction on the molecular chaperone of endoplasmic reticulum in skeletal muscle and glucose metabolism. Methods ： 37 SD rats were divided into silent group （S）, dietsilent group （DS）, training group （T） and diettraining group （DT） to undertake swimming endurance training by increasing load, during which the D group＇s food intake was only 70% of the normal groups for four weeks. RT - PCR and western blotting were adopted respectively to detect skeletal muscle ER, stress protein GRP78 and the mRNA and protein expression of ORP150. Blood glucose（BG）, fasting serum insulin（FINS） , plasma glucagon（ GLU）, the glycogen（Gn） of quadrieeps femoris, hexokinase （ HK）, and the activity of pyruvate kinase（PK） were also detected. Results：（1） training and diet as well as training ＋ diet mildly increased GRP78 and ORP150 mRNA （＋ 24% to43% , P 〉 0.05） and protein expression （15% - 15%, P 〉 0.05） ; （2） training and diet significantly reduced body weight and body fat while training ＋ diet displayed more obvious effect; diet significantly reduced silence BG, training ＋ diet significantly slowed BG＇s recovery; training and diet significantly reduced the level of FINS while training ＋ diet reduced it more. Diet significantly increased insulin sensitivity （ISI） ; Training and training ＋ diet largely increased HK＇s activity and training ＋ diet obviously increased the Gn content; the expression of GRP78 and ORP150 mRNA exhibited significantly positive correlation and so did the protein expression of ORP150 and ISI followed by Gn and HK. Conclusion ： 4 weeks of increasing load swimming endurance training and moderate diet mildly increased the expression of skeletal muscle/ORP150 GRP78, the possible reason of which might be the reduction of BG and its low level of free sugar. Endurance training and moderate diet improves the sugar metabolism of skeletal muscle and endurance training combined with moderate diet could produce better effect.