北京体育大学学报
北京體育大學學報
북경체육대학학보
Journal of Beijing University of Physical Education
2013年
4期
49~52
,共null页
大黄提取物 代谢组学 血脂异常 有氧运动 SD大鼠
大黃提取物 代謝組學 血脂異常 有氧運動 SD大鼠
대황제취물 대사조학 혈지이상 유양운동 SD대서
Rhubarb ; metabonomics ; dyslipidemia ; aerobic exercise ; SD rats
目的：基于UPLC-代谢组学技术表征大黄结合有氧运动对血脂异常大鼠血脂的调节作用。方法：用高脂饲料喂饲大鼠4周，同时进行4周的大黄、有氧运动、大黄与运动结合干预。实验第1d和第2周末眼眶静脉丛取血，第4周后处死动物，采用基于UPLC-代谢组学证明不同治疗组的治疗效果。比较血清TC、TG、HDL-C、LDL-C、SOD、GSH-Px、CAT和MDA的变化。结果：代谢组学与生化指标的结果结合证明在4周时间，血脂异常模型被成功建立，不同的时间段产生不同的损伤程度且明确了血脂异常模型的时间进展轨迹，显示了不同治疗组的对血脂异常的影响。代谢组学显示不仅大黄结合有氧运动组对血脂异常的有一定的治疗作用，而且二者结合的治疗效果优于单一大黄组或有氧运动组。生化指标显示大黄和有氧运动均可显著降低大鼠血清TC、TG和LDL-C提高、HDL-C水平；大黄结合有氧运动组大鼠血清SOD、GSH-Px、CAT显著升高同时MDA的水平下降，脂质过氧化产物水平显著降低。结论：大黄结合有氧运动可治疗血脂异常大鼠血脂异常。代谢组学显示血脂异常模型已成功建立，进而可推广代谢组学技术可以作为一种推测药理模型制作程度及是否成功的新方法，代谢组学也作为药物或有氧运动改善疾病程度及效果的一个新方法。
目的：基于UPLC-代謝組學技術錶徵大黃結閤有氧運動對血脂異常大鼠血脂的調節作用。方法：用高脂飼料餵飼大鼠4週，同時進行4週的大黃、有氧運動、大黃與運動結閤榦預。實驗第1d和第2週末眼眶靜脈叢取血，第4週後處死動物，採用基于UPLC-代謝組學證明不同治療組的治療效果。比較血清TC、TG、HDL-C、LDL-C、SOD、GSH-Px、CAT和MDA的變化。結果：代謝組學與生化指標的結果結閤證明在4週時間，血脂異常模型被成功建立，不同的時間段產生不同的損傷程度且明確瞭血脂異常模型的時間進展軌跡，顯示瞭不同治療組的對血脂異常的影響。代謝組學顯示不僅大黃結閤有氧運動組對血脂異常的有一定的治療作用，而且二者結閤的治療效果優于單一大黃組或有氧運動組。生化指標顯示大黃和有氧運動均可顯著降低大鼠血清TC、TG和LDL-C提高、HDL-C水平；大黃結閤有氧運動組大鼠血清SOD、GSH-Px、CAT顯著升高同時MDA的水平下降，脂質過氧化產物水平顯著降低。結論：大黃結閤有氧運動可治療血脂異常大鼠血脂異常。代謝組學顯示血脂異常模型已成功建立，進而可推廣代謝組學技術可以作為一種推測藥理模型製作程度及是否成功的新方法，代謝組學也作為藥物或有氧運動改善疾病程度及效果的一箇新方法。
목적：기우UPLC-대사조학기술표정대황결합유양운동대혈지이상대서혈지적조절작용。방법：용고지사료위사대서4주，동시진행4주적대황、유양운동、대황여운동결합간예。실험제1d화제2주말안광정맥총취혈，제4주후처사동물，채용기우UPLC-대사조학증명불동치료조적치료효과。비교혈청TC、TG、HDL-C、LDL-C、SOD、GSH-Px、CAT화MDA적변화。결과：대사조학여생화지표적결과결합증명재4주시간，혈지이상모형피성공건립，불동적시간단산생불동적손상정도차명학료혈지이상모형적시간진전궤적，현시료불동치료조적대혈지이상적영향。대사조학현시불부대황결합유양운동조대혈지이상적유일정적치료작용，이차이자결합적치료효과우우단일대황조혹유양운동조。생화지표현시대황화유양운동균가현저강저대서혈청TC、TG화LDL-C제고、HDL-C수평；대황결합유양운동조대서혈청SOD、GSH-Px、CAT현저승고동시MDA적수평하강，지질과양화산물수평현저강저。결론：대황결합유양운동가치료혈지이상대서혈지이상。대사조학현시혈지이상모형이성공건립，진이가추엄대사조학기술가이작위일충추측약리모형제작정도급시부성공적신방법，대사조학야작위약물혹유양운동개선질병정도급효과적일개신방법。
Objective： UPLC-based metabonomics study on therapeutic effect of Rhubarb and aerobic exercise in dyslipidemia rat. Methods ： The rats were raised by high fat diet for 4 weeks ; meanwhile these rats were treated with Rhubarb and aerobic exercise for 4 weeks. The blood samples were collected from retro-orbital puncture with ether and transferred into tubes on the first day and the second weekend. Then those rats were executed, and the model of dyslipidemia was then established and the model was verified by metabonomics method. The serum TC, TG, LDL-C, HDL-C, GSH-Px, SOD, CAT and MDA were determined. Results： According to metabonomie method and biochemical parameters, the hyperlipemia rat model was successfully established in 4 weeks. The re- sults showed the time progress of metabolic disorders in the different periods. The results showed that Rhubarb and aerobic exercise could decrease the concentration of serum LDL-C, TC and TG remarkably and raise the concen- tration of HDL-C. The activity of GSH-Px, SOD and CAT in Rhubarb in combination with aerobic exercise group is promoted and the level of MAD is decreased distinctly. Conclusion： Rhubarb in combination with aerobic exer- cise could treat the abnormity of serum lipid and has obviously anti-lipoperoxidation effect. The metabonomic re- sult showed the model of dyslipidemia was then established. The metabolomics technology can be used as a new method whether successful pharmacology model established or not. Metabolomics could also be a new method for studying on the therapeutic effect of aerobic exercise.
