CAJ | 학술논문

目的：拟建立一次性大强度耐力运动动物模型，观察AMPK活性变化对Akt-mTOR信号通路的影响。方法：36只sD大鼠进行1次跑台运动，坡度5％，运动强度25m／min，运动时间60min。取样为运动前、运动0．5h、运动1h、运动后1h、运动后2h、运动后6h等6个点，每个采样点6只大鼠。采用同位素技术测定腓肠肌中AMPK活性的变化，采用Westernblot方法，测定Akt、mTOR总蛋白含量及其磷酸化变化。结果：1）与安静组相比，运动各组AMPK活性都升高，有显著性或非常显著性差异（P〈0．05或P〈0．01），AMPK活性在运动0．5h后开始升高，运动后1h达到最高，运动后6h开始下降但还高于安静组；2）与安静组相比，运动各组Akt、mTOR总蛋白含量没有显著性差异；3）与安静组相比，Akt^ser473。磷酸化在运动0．5h组显著升高（P〈0．05），在运动1h组非常显著性升高（P〈0．01），而在运动后1h组有下降趋势，但没有显著性差异，在运动后2h、6h组非常显著性下降（p〈0．01）；4）与安静组相比，mTOR^ser448。磷酸化在运动0．5h，1h组显著性升高（P〈0．05）；而在运动后1h、2h、6h组mTOR^ser448。磷酸化显著性下降（P〈0．05）。结论：1）AMPK活性升高，可能通过降低Akt^ser473磷酸化，抑制Akt-mTOR信号通路。2）一次性大强度耐力运动中，Akt^ser473磷酸化除受AMPK调节外，可能还受其它因素调节。
목적：의건립일차성대강도내력운동동물모형，관찰AMPK활성변화대Akt-mTOR신호통로적영향。방법：36지sD대서진행1차포태운동，파도5％，운동강도25m／min，운동시간60min。취양위운동전、운동0．5h、운동1h、운동후1h、운동후2h、운동후6h등6개점，매개채양점6지대서。채용동위소기술측정비장기중AMPK활성적변화，채용Westernblot방법，측정Akt、mTOR총단백함량급기린산화변화。결과：1）여안정조상비，운동각조AMPK활성도승고，유현저성혹비상현저성차이（P〈0．05혹P〈0．01），AMPK활성재운동0．5h후개시승고，운동후1h체도최고，운동후6h개시하강단환고우안정조；2）여안정조상비，운동각조Akt、mTOR총단백함량몰유현저성차이；3）여안정조상비，Akt^ser473。린산화재운동0．5h조현저승고（P〈0．05），재운동1h조비상현저성승고（P〈0．01），이재운동후1h조유하강추세，단몰유현저성차이，재운동후2h、6h조비상현저성하강（p〈0．01）；4）여안정조상비，mTOR^ser448。린산화재운동0．5h，1h조현저성승고（P〈0．05）；이재운동후1h、2h、6h조mTOR^ser448。린산화현저성하강（P〈0．05）。결론：1）AMPK활성승고，가능통과강저Akt^ser473린산화，억제Akt-mTOR신호통로。2）일차성대강도내력운동중，Akt^ser473린산화제수AMPK조절외，가능환수기타인소조절。
Objective ： The authors have probed into the effects of AMPK activation on Akt-mTOR signal pathway a- bout protein synthesis in rat skeletal muscle through the one-time high intensity exercise. Methods： 36 SD rats do a one-time treadmill exercise at a slope of 5% , an exercise intensity of 25 m/min, and an exercise time of 60 rain. The sampling times are different before exercise, after 0.5 h, 1 h of exercise, 1 h after exercise, 2 h after exercise and 6 h after exercise. The authors measured the changes of AMPK activity in gastrocnemius muscle by u- sing isotope technology. Phosphorylation mTORs^ser2448 was determined by western blot, as well as total Akt, total mTOR,phosphorylation Akts^ser473. Results： 1 ） The AMPK activity started to increase after 0. 5 h of exercise, reached its peak at 1 h after exercise, started to lower at 6 h after exercise but was still higher than that of rats in the control group ;2）In the course of exercise, Akt, roTOR total protein content, among the groups there were no significant differences. 3 ） Compared with those of rats in the control group, Akts^ser473 phosphorylation of rats in 0.5 h and 1 h exercise groups increased respectively, and the differences were significant or very significant （P 〈 0.05, P 〈0.01 ） ; compared with those of rats in the control group, AktS^ser473 phosphorylation of rats in the 2 h and 6 h af- ter exercise group decreased, and the differences were very significant （P 〈 0.01 ）. 4） Compared with those of rats in the control group, mTOR^ser448 phosphorylation of rats in 0.5 h and 1 h exercise groups increased respectively, and the differences were significant （P 〈 0.05 ） ; Compared with those of rats in the control group, mTOR^ser448 phospho-rylation of rats in the 1 h,2 h and 6 h after exercise group decreased, and the differences were significant （ P 〈 0.05）. Conclusion： 1 ） The activated AMPK may inhibit the Akt-mTOR signal pathway through decreasing Akt phosphorylation. 2） During one-time high intensity endurance exercise, Akt^ser473 phosphate may regulate by AMPK, and may also be affected by the other factors.