CAJ | 학술논문

联吡啶钌是一种电化学发光试剂，对乙酰氨基酚对其电致发光信号有增敏作用．基于此原理，建立了毛细管电泳电化学发光分析方法用于测定对乙酰氨基酚．讨论了磷酸盐缓冲液pH值、浓度等实验参数对于对乙酰氨基酚检测的影响．在最佳实验条件下，检测电位1．15V；钌联吡啶浓度5mmol／L；检测池缓冲溶液50mmol／L（pH7．5）；流动缓冲液50mmol／L（pH7．5）；进样高压10kV；进样时间8s，运行高压12kV，在3min内可实现对乙酰氨基酚的分离检测，对乙酰氨基酚在0．05～10mg／L内呈良好线性，检出限为0．01rag／L．对1．0mg／L对乙酰氨基酚进行7次重复测定，所得电化学发光信号和迁移时间的RSD分别为3．5％和1．6％（n=7）．在优化的实验条件下，应用此法测定了实际样品中对乙酰氨基酚的含量，回收率为92％～105％．
련필정조시일충전화학발광시제，대을선안기분대기전치발광신호유증민작용．기우차원리，건립료모세관전영전화학발광분석방법용우측정대을선안기분．토론료린산염완충액pH치、농도등실험삼수대우대을선안기분검측적영향．재최가실험조건하，검측전위1．15V；조련필정농도5mmol／L；검측지완충용액50mmol／L（pH7．5）；류동완충액50mmol／L（pH7．5）；진양고압10kV；진양시간8s，운행고압12kV，재3min내가실현대을선안기분적분리검측，대을선안기분재0．05～10mg／L내정량호선성，검출한위0．01rag／L．대1．0mg／L대을선안기분진행7차중복측정，소득전화학발광신호화천이시간적RSD분별위3．5％화1．6％（n=7）．재우화적실험조건하，응용차법측정료실제양품중대을선안기분적함량，회수솔위92％～105％．
A simple method is developed for the determination of paracetamol by electrochemi- lurninescence（ECL）detection with the aid of capillary electrophoresis, which is based on the fact that the ECL intensity of Ru（bpy）2 3 ＋ can be enhanced in the presence of paracetamol in PBS medium. Sample solution is injected to separation capillary of 40 cm length （25 μm i.d.） by electrokinetic iniection for 8 s at 10 kV. Under the optimized condition, the linear range from 0.05-10 mg/L for paracetamol and detection limit （S/N=3） of 0.1mg/L is achieved. The RSD of migration time and ECL intensity for continuous injections of lmg/L paracetamol （n = 7） are 1.6% and 3.5%, respectively.This method has been applied to the analysis of paracetamol in real samples, and the recovery ranged from 92% to 105%.