CAJ | 학술논문

目的：1）观察3 d低氧暴露、耐力运动对大鼠肺组织TF、TFPI-1 mRNA表达的影响。2）通过TF/TFPI-1的比值变化来观察机体凝血状态的变化趋势。方法：6周龄雄性SD大鼠32只,随机分为4组：常氧安静组、常氧耐力运动组、低氧安静组、低氧耐力运动组。采用常压低氧舱以13.6%的氧浓度（相当于海拔3 500 m的氧浓度）进行低氧训练,低氧训练强度为30 m/min,常氧耐力运动强度为35 m/min,持续运动1 h/d,训练3 d。采用实时荧光定量PCR技术测试大鼠肺组织TF、TFPI-1 mRNA表达的变化。结果：1）低氧安静组的TF、TFPI-1 mRNA表达较常氧安静组相比均有非常显著性升高（P〈0.01）,TF/TFPI-1有显著性升高（P〈0.05）;2）低氧耐力运动组的TF、TFPI-1 mRNA表达较常氧安静组有显著性升高（P〈0.05）,但TF/TFPI-1无明显差异;3）低氧耐力运动组的TF、TFPI-1 mRNA表达及TF/TFPI-1 3者较均低氧安静组有显著性下降（P〈0.05）。结论：1）大鼠在13.6%的低氧环境下暴露3 d后,可促进血液凝血;2）3 d低氧耐力运动可降低低氧暴露对血液凝血状态的影响程度,提示高原耐力运动可降低或避免血液凝血状态的紊乱。
목적：1）관찰3 d저양폭로、내력운동대대서폐조직TF、TFPI-1 mRNA표체적영향。2）통과TF/TFPI-1적비치변화래관찰궤체응혈상태적변화추세。방법：6주령웅성SD대서32지,수궤분위4조：상양안정조、상양내력운동조、저양안정조、저양내력운동조。채용상압저양창이13.6%적양농도（상당우해발3 500 m적양농도）진행저양훈련,저양훈련강도위30 m/min,상양내력운동강도위35 m/min,지속운동1 h/d,훈련3 d。채용실시형광정량PCR기술측시대서폐조직TF、TFPI-1 mRNA표체적변화。결과：1）저양안정조적TF、TFPI-1 mRNA표체교상양안정조상비균유비상현저성승고（P〈0.01）,TF/TFPI-1유현저성승고（P〈0.05）;2）저양내력운동조적TF、TFPI-1 mRNA표체교상양안정조유현저성승고（P〈0.05）,단TF/TFPI-1무명현차이;3）저양내력운동조적TF、TFPI-1 mRNA표체급TF/TFPI-1 3자교균저양안정조유현저성하강（P〈0.05）。결론：1）대서재13.6%적저양배경하폭로3 d후,가촉진혈액응혈;2）3 d저양내력운동가강저저양폭로대혈액응혈상태적영향정도,제시고원내력운동가강저혹피면혈액응혈상태적문란。
Objective： 1 ） To investigate the effect of hypoxic training on TF, TFPI-1 mRNA expression in rats＇ lung. 2） Through the ratio of TF/TFPI-1, the researchers investigate the changing of blood clotting state. Meth- od： thirty-two male SD rats were selected from fifty and divided into 4 groups randomly： normoxic sedentary （NS）, hypoxie sedentary （HS）, normoxie training （NT）, bypoxic training （HT）. According to the groups, hy- poxic exposure groups performed training on a treadmill in normobaric hypoxic cabin （artificial normobaric hypoxia at 13.6% oxygen content, equal to 3 500 m altitude） , 30 m/rain, 1 h/d, 3 d, or normoxic training in a normoxic room in 35 m/min for the same duration. Tree days later, the lung samples were removed. For training groups, they were removed 24 hours after the last training period. The changes of TF and TFPI-1 mRNA were tested by RT-PCR （real-time PCR）. Result： 1） Compared to NS, TF, TFPI-1 mRNA expression increased in HS（P 〈0. 01 ） , and the ratio of TF/TFPI-1 also significantly improved （P 〈0.05）. 2） Compared to NT, TF, TFPI-1 mR- NA expression increased in HT （P 〈 0.05 ） , but the ratio of TF/TFPI-1 had no significant difference. 3 ） Com- pared to HS, TF, TFPI-1 mRNA expression and the ratio of TF/TFPI-1 decreased in HT （ P 〈 0.05 ）. Conclu- sion ： 1 ） Three-day of acute hypoxic exposure could promote blood clotting state. 2 ） Three-day hypoxic training could reduce the degree of blood clotting state, which provided theory basis that hypoxic training could avoid or re-duce blood coagulation disorders.