CAJ | 학술논문

极低频电磁场能加快组织愈合、促进细胞分化等，但是对骨骼肌钝挫伤后恢复过程中的作用研究甚少。用极低频电磁场对骨骼肌钝挫伤大鼠模型进行处理，并通过组织学观察及生理、生化相关指标检测来分析判断ELFMF对骨骼肌损伤恢复的影响。结果发现，与假暴露组相比，磁场暴露组肌细胞排列更整齐，炎性细胞浸润少，细胞水肿程度轻，核内移现象少；磁场暴露组、注射bFGF组肌纤维排列紊乱程度、Z线飘移程度均较轻；极低频电磁场处理，能显著降低钝挫伤后血清CK、LDH及ALP水平。因此，极低频电磁场通过改变钝挫伤后腓肠肌肌细胞核增生，加快线粒体自身修复速度，减少肌细胞膜的通透性，加速受损肌细胞有氧代谢能力的恢复。极低频电磁场通过影响肌细胞核及线粒体的增生，缓解受损骨骼肌的能量危机，从而加速受损肌组织恢复速度。
겁저빈전자장능가쾌조직유합、촉진세포분화등，단시대골격기둔좌상후회복과정중적작용연구심소。용겁저빈전자장대골격기둔좌상대서모형진행처리，병통과조직학관찰급생리、생화상관지표검측래분석판단ELFMF대골격기손상회복적영향。결과발현，여가폭로조상비，자장폭로조기세포배렬경정제，염성세포침윤소，세포수종정도경，핵내이현상소；자장폭로조、주사bFGF조기섬유배렬문란정도、Z선표이정도균교경；겁저빈전자장처리，능현저강저둔좌상후혈청CK、LDH급ALP수평。인차，겁저빈전자장통과개변둔좌상후비장기기세포핵증생，가쾌선립체자신수복속도，감소기세포막적통투성，가속수손기세포유양대사능력적회복。겁저빈전자장통과영향기세포핵급선립체적증생，완해수손골격기적능량위궤，종이가속수손기조직회복속도。
Magnetic field therapy is suggested to enhance healing of injuries and promote cell differentiation, but lack of researches on skeletal muscle contusion in the restoration process of action. In this study we used extremely low frequency magnetic fields （ELFMFs） on skeletal muscle contusion in rat model, and by histological observation and physiological, biochemical indexes related to detection to analyze the ELFMF effects on skeletal muscle injury recovery. Compared with the natural control animals, the microcytes of sham animals showed no obvious mitochondria structure as well as myofibrils blurred and Z -line aligned disorderly. Length differences of sarcomere among the bFGF group, the exposed group and the sham group were significant （P 〈 0.05 ）. In terms of histochemistry, the exposed group and the injection group showed better recovery than the sham group. In terms of biochemistry, ferritin and transferrin in serum or tissue of individual group were determined and analyzed. The detailed mechanisms involved were still under investigation. ELFMFs could change contusion gastrocnemius muscle cell nuclei hyperplasia, accelerate mitochondrial repair speed, reduce muscle cell membrane permeability and accelerate the recovery rate of aerobic capacity in muscle cells. Therefore,ELFMFs can influence the regeneration of nuclei and mitochondria in iinpaired skeletal muscle, alleviate the energy crisis,inorder to accelerated the damaged muscle tissue recovery rate.