天津体育学院学报
天津體育學院學報
천진체육학원학보
Journal of Tianjin Institute of Physical Education
2013年
2期
180~184
,共null页
HSPA1A基因 多态性,单核苷酸 有氧运动能力
HSPA1A基因 多態性,單覈苷痠 有氧運動能力
HSPA1A기인 다태성,단핵감산 유양운동능력
HSPA1A;Polymorphism;single nucleotide;aerobic capacity
目的：探讨热休克蛋白70（Heat Shock Protein 70,HSP70）基因（HSPA1A）＋190G〉C单核苷酸多态性（Single Nucleotide Polymorphism,SNP）与有氧运动能力表型指标的关系。方法：试验对象为400名大学一年级学生,其中男生300名,女生100名,均为汉族。受试者以95%~105%个体通气无氧阈（VT）进行每周3次,每次5 000 m,为期18天的长跑训练。测定训练前后摄氧量绝对值（VO2）、摄氧量相对值（rVO2）、通气无氧阈（VT）和每分钟通气量（VE）,并进行跑节省化试验。采用ABI7900HT的Taqman基因分型技术,检测HSP1A1基因＋190G〉C位点基因型。结果：HSP1A1＋190G〉C位点基因多态性与有氧运动能力表型存在关联,随着G等位基因的增加,个体有氧运动能力也在增加;有氧耐力训练前后,不同基因型者VO2max、rVO2max、VT/VO2和VE均无统计学差异（P〉0.05）;有氧耐力训练前GG基因型个体RE/HR值显著低于GC和CC基因型者,且随着C基因型的增加,RE/HR值也逐渐增加,趋势检验发现具有剂量效应关系（Ptrend〈0.05）;有氧耐力训练后不同基因型RE/HR和RE/VE值以及他们的变化率间差异具有统计学意义（P〈0.05）,且随着C基因型的增加,RE/HR和RE/VE值以及他们的变化率也逐渐增加,趋势检验发现具有剂量效应关系（Ptrend〈0.05）。结论：HSPA1A基因＋190G〉C位点与有氧耐力训练后有氧运动能力表型存在关联,GG基因型个体具有较高的有氧运动能力水平。
目的：探討熱休剋蛋白70（Heat Shock Protein 70,HSP70）基因（HSPA1A）＋190G〉C單覈苷痠多態性（Single Nucleotide Polymorphism,SNP）與有氧運動能力錶型指標的關繫。方法：試驗對象為400名大學一年級學生,其中男生300名,女生100名,均為漢族。受試者以95%~105%箇體通氣無氧閾（VT）進行每週3次,每次5 000 m,為期18天的長跑訓練。測定訓練前後攝氧量絕對值（VO2）、攝氧量相對值（rVO2）、通氣無氧閾（VT）和每分鐘通氣量（VE）,併進行跑節省化試驗。採用ABI7900HT的Taqman基因分型技術,檢測HSP1A1基因＋190G〉C位點基因型。結果：HSP1A1＋190G〉C位點基因多態性與有氧運動能力錶型存在關聯,隨著G等位基因的增加,箇體有氧運動能力也在增加;有氧耐力訓練前後,不同基因型者VO2max、rVO2max、VT/VO2和VE均無統計學差異（P〉0.05）;有氧耐力訓練前GG基因型箇體RE/HR值顯著低于GC和CC基因型者,且隨著C基因型的增加,RE/HR值也逐漸增加,趨勢檢驗髮現具有劑量效應關繫（Ptrend〈0.05）;有氧耐力訓練後不同基因型RE/HR和RE/VE值以及他們的變化率間差異具有統計學意義（P〈0.05）,且隨著C基因型的增加,RE/HR和RE/VE值以及他們的變化率也逐漸增加,趨勢檢驗髮現具有劑量效應關繫（Ptrend〈0.05）。結論：HSPA1A基因＋190G〉C位點與有氧耐力訓練後有氧運動能力錶型存在關聯,GG基因型箇體具有較高的有氧運動能力水平。
목적：탐토열휴극단백70（Heat Shock Protein 70,HSP70）기인（HSPA1A）＋190G〉C단핵감산다태성（Single Nucleotide Polymorphism,SNP）여유양운동능력표형지표적관계。방법：시험대상위400명대학일년급학생,기중남생300명,녀생100명,균위한족。수시자이95%~105%개체통기무양역（VT）진행매주3차,매차5 000 m,위기18천적장포훈련。측정훈련전후섭양량절대치（VO2）、섭양량상대치（rVO2）、통기무양역（VT）화매분종통기량（VE）,병진행포절성화시험。채용ABI7900HT적Taqman기인분형기술,검측HSP1A1기인＋190G〉C위점기인형。결과：HSP1A1＋190G〉C위점기인다태성여유양운동능력표형존재관련,수착G등위기인적증가,개체유양운동능력야재증가;유양내력훈련전후,불동기인형자VO2max、rVO2max、VT/VO2화VE균무통계학차이（P〉0.05）;유양내력훈련전GG기인형개체RE/HR치현저저우GC화CC기인형자,차수착C기인형적증가,RE/HR치야축점증가,추세검험발현구유제량효응관계（Ptrend〈0.05）;유양내력훈련후불동기인형RE/HR화RE/VE치이급타문적변화솔간차이구유통계학의의（P〈0.05）,차수착C기인형적증가,RE/HR화RE/VE치이급타문적변화솔야축점증가,추세검험발현구유제량효응관계（Ptrend〈0.05）。결론：HSPA1A기인＋190G〉C위점여유양내력훈련후유양운동능력표형존재관련,GG기인형개체구유교고적유양운동능력수평。
Objective：To explore the associations between the heat shock protein 70 gene（HSPA1A）＋190G＆gt;C polymorphisms and the aerobic capacity to aerobic endurance training program among healthy freshmen.Methods：A total of 400 healthy freshmen were included in the present study,including 300 boys and 100 girls and all were Han nationality.All subjects performed an aerobic endurance training program of 18-week 5000m running,3 times a week with the intensity of 95%-105% aerobic threshold.VO2max,rVO2max,VE,VT and VE were measured before and after the aerobic endurance training.Polymorphisms of HSP1A1 ＋190G＆gt;C were detected by Taqman single nucleotide polymorphism（SNP）allelic discrimination by means of an ABI7900HT.Results：The ＋190G＆gt;C SNP in HSPA1A gene was associated with aerobic capacity in men and women,individual aerobic capacity is also increasing with the increase of the G allele.People with the HSPA1A GG genotype have lower RE/HR values,than carriers of the HSPA1A C190 allele after the endurance training（P＆lt;0.05）,and there was a dose response relationship among the three genotype groups（Ptrend＆lt;0.05）.After the endurance training the RE/HR and RE/VE values were significant reduced,and these values in GG genotype were significant lower than the C allele,and there were also dose response relationships among the three genotype groups（Ptrend＆lt;0.05）.Conclusion：The ＋190G＆gt;C SNP in HSPA1A gene was not only associated with aerobic capacity but also with endurance training promotion.
