天津体育学院学报
天津體育學院學報
천진체육학원학보
Journal of Tianjin Institute of Physical Education
2013年
1期
8~11
,共null页
刘晓莉 陈艳芬 朱成才 乔德才
劉曉莉 陳豔芬 硃成纔 喬德纔
류효리 진염분 주성재 교덕재
运动疲劳 海马CA1区 学习记忆 长时程增强 钙神经素
運動疲勞 海馬CA1區 學習記憶 長時程增彊 鈣神經素
운동피로 해마CA1구 학습기억 장시정증강 개신경소
exercise induced fatigue; hippocampus CA1 area; learning and memory function; LTP; CaN
目的：通过观察运动疲劳对大鼠学习记忆、海马CA1区长时程增强（LTP）效应和钙神经素（CaN）蛋白表达的影响,探讨运动疲劳影响学习记忆功能的生理机制。方法：32只健康雄性8周鼠龄SD大鼠,随机分为安静对照组（CG）和运动疲劳组（FG）,每组16只。运动疲劳组做连续7天递增负荷跑台运动,每天运动至力竭,对照组不加干预。7天建模结束后,运用避暗穿梭实验检测2组大鼠学习记忆能力,并分别采用在体脑立体定位—神经电生理方法观察海马CA1区LTP效应和免疫组织化学方法观察大鼠海马CA1区CaN蛋白表达水平。结果：与CG大鼠相比,FG大鼠避暗潜伏期缩短,错误次数增加,且差异显著（P〈0.01）;最大场兴奋性突出后电位（fEPSP）的平均幅值FG大鼠（113.81%±2.36%）较CG大鼠（129.31%±2.38%）显著降低（P〈0.01）;FG大鼠CaN蛋白表达也较对照组显著上调（P〈0.01）。结论：运动疲劳可降低大鼠学习记忆能力,抑制海马CA1区LTP效应,上调CaN蛋白表达。提示：运动疲劳降低大鼠学习记忆功能的生理机制可能与海马CA1区LTP抑制及CaN蛋白表达增多有关。
目的：通過觀察運動疲勞對大鼠學習記憶、海馬CA1區長時程增彊（LTP）效應和鈣神經素（CaN）蛋白錶達的影響,探討運動疲勞影響學習記憶功能的生理機製。方法：32隻健康雄性8週鼠齡SD大鼠,隨機分為安靜對照組（CG）和運動疲勞組（FG）,每組16隻。運動疲勞組做連續7天遞增負荷跑檯運動,每天運動至力竭,對照組不加榦預。7天建模結束後,運用避暗穿梭實驗檢測2組大鼠學習記憶能力,併分彆採用在體腦立體定位—神經電生理方法觀察海馬CA1區LTP效應和免疫組織化學方法觀察大鼠海馬CA1區CaN蛋白錶達水平。結果：與CG大鼠相比,FG大鼠避暗潛伏期縮短,錯誤次數增加,且差異顯著（P〈0.01）;最大場興奮性突齣後電位（fEPSP）的平均幅值FG大鼠（113.81%±2.36%）較CG大鼠（129.31%±2.38%）顯著降低（P〈0.01）;FG大鼠CaN蛋白錶達也較對照組顯著上調（P〈0.01）。結論：運動疲勞可降低大鼠學習記憶能力,抑製海馬CA1區LTP效應,上調CaN蛋白錶達。提示：運動疲勞降低大鼠學習記憶功能的生理機製可能與海馬CA1區LTP抑製及CaN蛋白錶達增多有關。
목적：통과관찰운동피로대대서학습기억、해마CA1구장시정증강（LTP）효응화개신경소（CaN）단백표체적영향,탐토운동피로영향학습기억공능적생리궤제。방법：32지건강웅성8주서령SD대서,수궤분위안정대조조（CG）화운동피로조（FG）,매조16지。운동피로조주련속7천체증부하포태운동,매천운동지력갈,대조조불가간예。7천건모결속후,운용피암천사실험검측2조대서학습기억능력,병분별채용재체뇌입체정위—신경전생리방법관찰해마CA1구LTP효응화면역조직화학방법관찰대서해마CA1구CaN단백표체수평。결과：여CG대서상비,FG대서피암잠복기축단,착오차수증가,차차이현저（P〈0.01）;최대장흥강성돌출후전위（fEPSP）적평균폭치FG대서（113.81%±2.36%）교CG대서（129.31%±2.38%）현저강저（P〈0.01）;FG대서CaN단백표체야교대조조현저상조（P〈0.01）。결론：운동피로가강저대서학습기억능력,억제해마CA1구LTP효응,상조CaN단백표체。제시：운동피로강저대서학습기억공능적생리궤제가능여해마CA1구LTP억제급CaN단백표체증다유관。
Objective： To observe the effects of exercise induced fatigue on learning and memory function,long-term potentiation and calcineurin protein expression of rats in hippocampal CA1 area and discuss the physiological mechanism of exercise-induced fatigue on learning and memory function.Methods： 32 healthy male SD rats at 8 weeks age were randomly divided into two groups（control group and fatigue group）.Seven days of increasing load exercise to build exercise-induced fatigue model.Control group was no intervention.After the end of last running,dark avoidance test was used to detect the changes of learning and memory function.At the end of the experiment,stereotaxic neuronal electrophysiological methods in vivo and immunohistochemistry were used to record LTP and observe the distribution and expression of CaN in hippocampus CA1 area.Results： Compared with CG rats,FG rats showed shorted latency time and increased numbers of error（P0.01）.FG rats average level of fEPSP（113.81 % ± 2.36 %）was lower than CG rats（129.31 %±2.38 %）（P0.01）.It found that the level of CaN was up-expressed in rats of fatigue group（P0.01）.Conclusion： Exercise-induced fatigue reduced the learning and memory abilities significantly,inhibited LTP in hippocampus CA1 and up-expressed the level of CaN.The results indicate that the physiological mechanism of exercise-induced fatigue reducing learning and memory function may be related to the inhibition of LTP and the increased expression of CaN protein in hippocampus.
