山东体育学院学报
山東體育學院學報
산동체육학원학보
Journal of Shandong Institute Of Physical Education And Sports
2013年
4期
46~51
,共null页
白藜芦醇 大鼠 脊髓损伤 神经细胞凋亡 通路
白藜蘆醇 大鼠 脊髓損傷 神經細胞凋亡 通路
백려호순 대서 척수손상 신경세포조망 통로
resveratrol ; rats ; spinal cord injury; apoptosis of nerve cells; signal transduction path-ways
目的：探讨白藜芦醇（Resveratrol，Res）对大鼠脊髓损伤（Spinalcordinjury，SCI）后细胞凋亡及通路的影响。方法采用Alien’s脊髓打击损伤模型（T8），将27只SD大鼠随机分为假手术组（Sham组）；损伤组（Control组）；白藜芦醇处理组（Res组），每组9只。术后72h，应用透射电镜观察脊髓组织细胞凋亡形态变化，免疫组化及Westernblot等方法检测脊髓组织中凋亡蛋白表达的变化。结果：透射电镜观察显示Res能明显抑制脊髓神经细胞的凋亡。免疫组化及Westernblot检测显示Sham组Bcl-2、Bax呈极少量表达；Control组Bax大量表达（P〈0．05），Bcl-2表达则较低（P〈0．05），两者比例失调，p38MAPK及Caspase-3明显激活（P〈0．05），神经细胞凋亡增加显著；与Control组相比，Res组Bax蛋白表达明显降低（P〈0．05），Bcl-2表达则有所增高（P〈0．05），p38MAPK及Caspase-3的激活明显受到抑制（P〈0．05），脊髓组织超微结构损害有所改善，神经细胞凋亡显著减少。结论：Res通过提高Bcl-2／Bax蛋白表达比，抑制p38MAPK及Caspase-3的激活，使大鼠脊髓组织超微结构损害有所改善，神经细胞凋亡显著减少，并且可阻断p38MAPK介导的信号转导通路。提示Res对大鼠SCI组织的神经细胞凋亡有较好的调控作用。
目的：探討白藜蘆醇（Resveratrol，Res）對大鼠脊髓損傷（Spinalcordinjury，SCI）後細胞凋亡及通路的影響。方法採用Alien’s脊髓打擊損傷模型（T8），將27隻SD大鼠隨機分為假手術組（Sham組）；損傷組（Control組）；白藜蘆醇處理組（Res組），每組9隻。術後72h，應用透射電鏡觀察脊髓組織細胞凋亡形態變化，免疫組化及Westernblot等方法檢測脊髓組織中凋亡蛋白錶達的變化。結果：透射電鏡觀察顯示Res能明顯抑製脊髓神經細胞的凋亡。免疫組化及Westernblot檢測顯示Sham組Bcl-2、Bax呈極少量錶達；Control組Bax大量錶達（P〈0．05），Bcl-2錶達則較低（P〈0．05），兩者比例失調，p38MAPK及Caspase-3明顯激活（P〈0．05），神經細胞凋亡增加顯著；與Control組相比，Res組Bax蛋白錶達明顯降低（P〈0．05），Bcl-2錶達則有所增高（P〈0．05），p38MAPK及Caspase-3的激活明顯受到抑製（P〈0．05），脊髓組織超微結構損害有所改善，神經細胞凋亡顯著減少。結論：Res通過提高Bcl-2／Bax蛋白錶達比，抑製p38MAPK及Caspase-3的激活，使大鼠脊髓組織超微結構損害有所改善，神經細胞凋亡顯著減少，併且可阻斷p38MAPK介導的信號轉導通路。提示Res對大鼠SCI組織的神經細胞凋亡有較好的調控作用。
목적：탐토백려호순（Resveratrol，Res）대대서척수손상（Spinalcordinjury，SCI）후세포조망급통로적영향。방법채용Alien’s척수타격손상모형（T8），장27지SD대서수궤분위가수술조（Sham조）；손상조（Control조）；백려호순처리조（Res조），매조9지。술후72h，응용투사전경관찰척수조직세포조망형태변화，면역조화급Westernblot등방법검측척수조직중조망단백표체적변화。결과：투사전경관찰현시Res능명현억제척수신경세포적조망。면역조화급Westernblot검측현시Sham조Bcl-2、Bax정겁소량표체；Control조Bax대량표체（P〈0．05），Bcl-2표체칙교저（P〈0．05），량자비례실조，p38MAPK급Caspase-3명현격활（P〈0．05），신경세포조망증가현저；여Control조상비，Res조Bax단백표체명현강저（P〈0．05），Bcl-2표체칙유소증고（P〈0．05），p38MAPK급Caspase-3적격활명현수도억제（P〈0．05），척수조직초미결구손해유소개선，신경세포조망현저감소。결론：Res통과제고Bcl-2／Bax단백표체비，억제p38MAPK급Caspase-3적격활，사대서척수조직초미결구손해유소개선，신경세포조망현저감소，병차가조단p38MAPK개도적신호전도통로。제시Res대대서SCI조직적신경세포조망유교호적조공작용。
Objective ： To study the effect of resveratrol injection on apoptosis of nerve cells and signal transduction pathways after spinal cord injuries in Rats. Methods ： Rat model of spinal cord （T8） in-jury according to the model of Allen＇s weight hit. 27 rats were randomly assigned into three groups （ n = 9 ） ： Sham group, Control group, Res group. At 72 hours, the morphological changes of apoptotic neurons were observed by electron microscopic; the Bcl-2, Bax and Caspase-3 and p38MAPK expression were observed by immunohistochemical staining and detected by Western blot analysis. Results：Resveratrol treatment inhibited injury-induced apoptosis as assessed by electrical microsco-py; the results of immunohistochemical staining and Western blot that the Bcl - 2 and Bax protein expression in the spinal cord tissue of sham - operated group was low. Howev-er, in the control group, Bax protein was higher and Bcl -2 protein was lower than that in sham group （ P 〈 O. 05 ）. There was a disproportion between the two proteins. We also found that the Caspase -3 and p38MAPK were significantly activated, and the neuronal apoptosis increased signifi-cantly. Compared to the control group, the expression of Bax protein in resveratrol group was signifi- cantly reduced （P 〈 0.05 ） and the Bcl - 2 protein expression increased significantly （ P 〈 0.05 ）. The activation of Caspase-3 and p38MAPK was inhibited （P 〈 0.05 ）. The ultrastructure injury of spinal cord tissue was improved and the neuronal apoptosis decreased significantly. Conclusion： Res-veratrol can prevent apoptosis of nerve cells after spinal cord injury by elevating the ratio of Bcl - 2/ Bax, inhibiting the activation of Caspase-3 and p38MAPK and improving uhrastructure of spinal cord tissue. All the results above indicate that Res has protective effects which may exert through modulating apoptosis of spinal cord after spinal cord injury.
