上海体育学院学报
上海體育學院學報
상해체육학원학보
Journal of Shanghai Physical Education Institute
2013年
5期
68~72
,共null页
低氧 大鼠 皮层细胞 血管内皮生长因子 ELISA RT-PCR
低氧 大鼠 皮層細胞 血管內皮生長因子 ELISA RT-PCR
저양 대서 피층세포 혈관내피생장인자 ELISA RT-PCR
hypoxia; rat; cerebral cortex cell ; VEGF; ELISA;RT-PCR
观察不同氧浓度环境下不同时间处理对大脑皮层细胞分泌血管内皮生长因子（VEGF）及表达VEGFmRNA的影响。方法：原代培养SD大鼠大脑皮层细胞，5d后将低氧组细胞转移至低氧工作站，在1％和4％氧浓度环境下分别处理3h和6h，其相应的常氧对照组细胞则继续放置于c02孵箱中。采用ELISA法检测细胞培养液中VEGF蛋白含量，采用RT-PCR法检测细胞内VEGFmRNA表达水平。结果：与对照组相比，在4％和1％氧浓度环境下3h组细胞VEGFmRNA及蛋白表达无明显变化（P〉0．05），但均有增加趋势，而6h组引起VEGFmRNA及蛋白表达明显增高（P〈0．05），且1％氧浓度6h组增幅更大（P〈O．01）。结论：低氧可诱导大鼠大脑皮层细胞分泌VEGF和表达VEGFmRNA。且皮层细胞的反应性与低氧浓度、低氧刺激时间有关。
觀察不同氧濃度環境下不同時間處理對大腦皮層細胞分泌血管內皮生長因子（VEGF）及錶達VEGFmRNA的影響。方法：原代培養SD大鼠大腦皮層細胞，5d後將低氧組細胞轉移至低氧工作站，在1％和4％氧濃度環境下分彆處理3h和6h，其相應的常氧對照組細胞則繼續放置于c02孵箱中。採用ELISA法檢測細胞培養液中VEGF蛋白含量，採用RT-PCR法檢測細胞內VEGFmRNA錶達水平。結果：與對照組相比，在4％和1％氧濃度環境下3h組細胞VEGFmRNA及蛋白錶達無明顯變化（P〉0．05），但均有增加趨勢，而6h組引起VEGFmRNA及蛋白錶達明顯增高（P〈0．05），且1％氧濃度6h組增幅更大（P〈O．01）。結論：低氧可誘導大鼠大腦皮層細胞分泌VEGF和錶達VEGFmRNA。且皮層細胞的反應性與低氧濃度、低氧刺激時間有關。
관찰불동양농도배경하불동시간처리대대뇌피층세포분비혈관내피생장인자（VEGF）급표체VEGFmRNA적영향。방법：원대배양SD대서대뇌피층세포，5d후장저양조세포전이지저양공작참，재1％화4％양농도배경하분별처리3h화6h，기상응적상양대조조세포칙계속방치우c02부상중。채용ELISA법검측세포배양액중VEGF단백함량，채용RT-PCR법검측세포내VEGFmRNA표체수평。결과：여대조조상비，재4％화1％양농도배경하3h조세포VEGFmRNA급단백표체무명현변화（P〉0．05），단균유증가추세，이6h조인기VEGFmRNA급단백표체명현증고（P〈0．05），차1％양농도6h조증폭경대（P〈O．01）。결론：저양가유도대서대뇌피층세포분비VEGF화표체VEGFmRNA。차피층세포적반응성여저양농도、저양자격시간유관。
Objectives： It is to observe the effect on the expressions of vascular endothelial growth factor （VEGF） mRNA and protein in cerebral cortex ceils under the environment of different oxygen concentration and time treatment. Methods： The primary cerebral cortex cells of SD rats were designed into two hypoxia groups and two normoxia control groups for 5 days. Then, the cells of hypoxia groups were treated by 3 hours （ 3 h） and 6 hours （ 6 h） under the environment of 1% and 4% oxygen concentration respectively in hypoxia workstation, while the corresponding control groups remained in HHCP. The ELASA method was used to detect VEGF protein content in cultural supernatants, and the level of intracellularVEGF mRNA was tested by RT-PCR. Results： Compared with the control groups, the level of VEGF mRNA and protein of 3 h groups had no significant difference under the environment of 1% and 4% oxygen concentration, but with a rising tendency; While the level of VEGF mRNA and protein of 6 h groups had significant rise, with a higher rise for the group under the environment of 1% oxygen concentration. Conclusions： Hypoxia can induce cerebral cortex cells of rats to produce VEGF and express VEGF mRNA; its reaction is related to the oxygen concentration and time treatment.
