CAJ | 학술논문

目的：探讨有氧运动训练对小鼠心脏形态结构、功能及心室肌微小RNAs（microRNAs，miRNAs）的影响，研究关健miRNA在心脏对运动适应过程中的调节作用。方法：KM雄性小鼠随机分为安静对照组和8周有氧运动训练组，每组各10只。有氧运动训练组小鼠进行为期8周的无负重有氧训练，每周训练5天，前5周，每天早上固定时间运动1次，后3周每天在早上和晚上固定时间分别运动1次；第1周每次运动时间为30m．in，随后每周增加10min直至90min。有氧运动训练完成后，首先使用超声心动图检测全部小鼠心脏各项形态学和功能学指标，随后将小鼠颈椎脱位猝死取心肌组织，使用基因芯片扫描两组差异表达的miRNAs，使用实时定量PCR技术验证111iRNA-1，-133a，-29和-30c4个关键差异表达的miRNA，同时使用荧光定量PCR和酶联免疫吸附技术检测小鼠心肌组织结缔组织生长因子（connectivetissuegrowthfactor，CTGF）基因和蛋白的表达。结果：有氧运动训练明显提高了小鼠心室内径、心壁厚度和功能学指标RatioE／A，提示运动诱导小鼠心脏生理性肥大，增加了心室顺应性；与安静对照组相比，8周有氧运动训练后小鼠心肌组织中miRNA-1和-133a显著下降（P〈0．05），而miRNA-29和-30c则显著升高（P〈0．05）；同时有氧运动训练组小鼠心肌组织中CTGF基因和蛋白的表达低于对照组，差异具有统计学意义（P〈0．05）；进-步研究发现，有氧运动训练组小鼠心肌组织中rniRNA-30c表达量与CTGF蛋白表达量呈负相关（相关系数r=-0．818，P=0．004）。结论：有氧运动训练通过增加心室肌组织中miRNA-30c的表达量，抑制心肌组织中CTGF的表达和浓度，从而提高运动引起心脏生理性肥大过程中心室顺应性，有利于心脏功能的正常有效发挥。
목적：탐토유양운동훈련대소서심장형태결구、공능급심실기미소RNAs（microRNAs，miRNAs）적영향，연구관건miRNA재심장대운동괄응과정중적조절작용。방법：KM웅성소서수궤분위안정대조조화8주유양운동훈련조，매조각10지。유양운동훈련조소서진행위기8주적무부중유양훈련，매주훈련5천，전5주，매천조상고정시간운동1차，후3주매천재조상화만상고정시간분별운동1차；제1주매차운동시간위30m．in，수후매주증가10min직지90min。유양운동훈련완성후，수선사용초성심동도검측전부소서심장각항형태학화공능학지표，수후장소서경추탈위졸사취심기조직，사용기인심편소묘량조차이표체적miRNAs，사용실시정량PCR기술험증111iRNA-1，-133a，-29화-30c4개관건차이표체적miRNA，동시사용형광정량PCR화매련면역흡부기술검측소서심기조직결체조직생장인자（connectivetissuegrowthfactor，CTGF）기인화단백적표체。결과：유양운동훈련명현제고료소서심실내경、심벽후도화공능학지표RatioE／A，제시운동유도소서심장생이성비대，증가료심실순응성；여안정대조조상비，8주유양운동훈련후소서심기조직중miRNA-1화-133a현저하강（P〈0．05），이miRNA-29화-30c칙현저승고（P〈0．05）；동시유양운동훈련조소서심기조직중CTGF기인화단백적표체저우대조조，차이구유통계학의의（P〈0．05）；진-보연구발현，유양운동훈련조소서심기조직중rniRNA-30c표체량여CTGF단백표체량정부상관（상관계수r=-0．818，P=0．004）。결론：유양운동훈련통과증가심실기조직중miRNA-30c적표체량，억제심기조직중CTGF적표체화농도，종이제고운동인기심장생이성비대과정중심실순응성，유리우심장공능적정상유효발휘。
Objective：To explore the effects of aerobic exercise training on the cardiac structure and miRNAs, and analyze their role in cardiac adaption induced by aerobic exercise training. Methods： Male KM mice （n= 10/group） were randomized into two groups： 8 weeks aerobic exercise training group and sedentary control group. Mice in training group undertook a swim- ming protocol for 8 weeks, 5days/wk, from the 1st to 5th week, mice were trained one time a day in the morning, from the 6th to 8th week, mice were trained twice a day with a 12 h inter- val. In the 1st week, the training time was 30 min, and the time was increased 10 min per week until to 90 min. After the aerobic exercise training, all mice were measured by echocar- diography. MiRNAs analysis was performed by miRNA microarray and confirmed by real-time PCR. The level of connective tissue growth factor （CTGF） gene expression was determined by real-time PCR, CTGF protein concentration was assessed by ELISA. Results：Aerobic exercise training improved aerobic capacity, left ventricular and induced cardiac hypertrophy. MiRNAs- 1 and 133 a were siginificant downregulated in training group （ P 〈 0.05 ）, however, miRNAs- 29c and 30c expression increased in training group （P〈0.05）, correlated with a decrease in CTGF gene expression in training group （P〈0.05）. Furthermore, miRNA-30 c was inversely correlated to CTGF protein contration in training group （r =-0.818, P= 0.05）. Conclusion： Aerobic exercise training could increase miRNA-30c expression and decrease CTGF gene ex- pression and concentration in the heart, which is relevant to the improved left ventricular com- pliance and beneficial cardiac effects.