中国体育科技
中國體育科技
중국체육과기
China Sport Science and Technology
2013年
5期
139~144
,共null页
乳腺癌 紫杉醇 运动 生存时间 细胞凋亡 核转录因子-κB 鼠 动物实验
乳腺癌 紫杉醇 運動 生存時間 細胞凋亡 覈轉錄因子-κB 鼠 動物實驗
유선암 자삼순 운동 생존시간 세포조망 핵전록인자-κB 서 동물실험
breast cancer; paclitaxel; exercise; survival time; apoptosis; NF-κB; rat; animnal experiment
目的:通过制备4T1小鼠乳腺癌移植模型,研究有氧运动、紫杉醇的单独及联合干预对小鼠4T1乳腺癌移植模型荷瘤生长的影响,并探讨其有关机制。方法:雌性6~8周龄BALB/c小鼠按体重随机分为正常对照组(N)和模型组。模型组小鼠接种4T1细胞。建模成功后,按体重、瘤大小分为生理盐水组(C)、紫杉醇组(P)、运动组(E)和联合组(EP)。P、EP组腹腔注射紫杉醇,剂量20mg/kg/w,其他组注射等体积生理盐水。运动强度采用17m/min,30min/次,5次/周,共5周。结果:1)EP组的瘤重、瘤体比、荷瘤体积均低于C组,差异显著(P〈0.05)。其他各组和C组相比,虽然瘤重低于C组,但均无显著性差异(P〉0.05)。2)P组、E组和EP组与C组相比,存活时间延长,有显著性差异(P〈0.05)。EP组与P组和E组相比,其延长生存时间的差异非常显著(P〈0.01)。3)与C组相比,EP组瘤细胞早、晚期凋亡比例增加,有显著性差异(P〈0.05)。4)与C组相比,EP组NF-κBp65的表达下降,差异非常显著(P〈0.01),其他各组与C组相比无显著差异(P〉0.05)。各组IκBα的表达无显著差异(P〉0.05)。与C组和P组相比,E、EP组的Phospho-IκBα的表达下降,差异非常显著(P〈0.01)。结论:中强度运动联合紫杉醇能抑制小鼠乳腺癌移植模型荷瘤的生长,延长其生存时间。减少癌细胞IκBα的磷酸化,调节NF-κB的活性,促进瘤细胞的凋亡,可能是运动联合紫杉醇影响小鼠乳腺癌移植模型荷瘤生长的机制之一。
目的:通過製備4T1小鼠乳腺癌移植模型,研究有氧運動、紫杉醇的單獨及聯閤榦預對小鼠4T1乳腺癌移植模型荷瘤生長的影響,併探討其有關機製。方法:雌性6~8週齡BALB/c小鼠按體重隨機分為正常對照組(N)和模型組。模型組小鼠接種4T1細胞。建模成功後,按體重、瘤大小分為生理鹽水組(C)、紫杉醇組(P)、運動組(E)和聯閤組(EP)。P、EP組腹腔註射紫杉醇,劑量20mg/kg/w,其他組註射等體積生理鹽水。運動彊度採用17m/min,30min/次,5次/週,共5週。結果:1)EP組的瘤重、瘤體比、荷瘤體積均低于C組,差異顯著(P〈0.05)。其他各組和C組相比,雖然瘤重低于C組,但均無顯著性差異(P〉0.05)。2)P組、E組和EP組與C組相比,存活時間延長,有顯著性差異(P〈0.05)。EP組與P組和E組相比,其延長生存時間的差異非常顯著(P〈0.01)。3)與C組相比,EP組瘤細胞早、晚期凋亡比例增加,有顯著性差異(P〈0.05)。4)與C組相比,EP組NF-κBp65的錶達下降,差異非常顯著(P〈0.01),其他各組與C組相比無顯著差異(P〉0.05)。各組IκBα的錶達無顯著差異(P〉0.05)。與C組和P組相比,E、EP組的Phospho-IκBα的錶達下降,差異非常顯著(P〈0.01)。結論:中彊度運動聯閤紫杉醇能抑製小鼠乳腺癌移植模型荷瘤的生長,延長其生存時間。減少癌細胞IκBα的燐痠化,調節NF-κB的活性,促進瘤細胞的凋亡,可能是運動聯閤紫杉醇影響小鼠乳腺癌移植模型荷瘤生長的機製之一。
목적:통과제비4T1소서유선암이식모형,연구유양운동、자삼순적단독급연합간예대소서4T1유선암이식모형하류생장적영향,병탐토기유관궤제。방법:자성6~8주령BALB/c소서안체중수궤분위정상대조조(N)화모형조。모형조소서접충4T1세포。건모성공후,안체중、류대소분위생리염수조(C)、자삼순조(P)、운동조(E)화연합조(EP)。P、EP조복강주사자삼순,제량20mg/kg/w,기타조주사등체적생리염수。운동강도채용17m/min,30min/차,5차/주,공5주。결과:1)EP조적류중、류체비、하류체적균저우C조,차이현저(P〈0.05)。기타각조화C조상비,수연류중저우C조,단균무현저성차이(P〉0.05)。2)P조、E조화EP조여C조상비,존활시간연장,유현저성차이(P〈0.05)。EP조여P조화E조상비,기연장생존시간적차이비상현저(P〈0.01)。3)여C조상비,EP조류세포조、만기조망비례증가,유현저성차이(P〈0.05)。4)여C조상비,EP조NF-κBp65적표체하강,차이비상현저(P〈0.01),기타각조여C조상비무현저차이(P〉0.05)。각조IκBα적표체무현저차이(P〉0.05)。여C조화P조상비,E、EP조적Phospho-IκBα적표체하강,차이비상현저(P〈0.01)。결론:중강도운동연합자삼순능억제소서유선암이식모형하류적생장,연장기생존시간。감소암세포IκBα적린산화,조절NF-κB적활성,촉진류세포적조망,가능시운동연합자삼순영향소서유선암이식모형하류생장적궤제지일。
Objective:The aim of this research is to study the effect of exercise conbined with paclitaxel on tumor growth in 4T1 Mouse Breast Cancer Model and discuss the mechanism.Methods:Six-to eight-week-old female BALB/c mice were randomly assigned into two groups of normal control(N,8animals)and model.For the transplantable tumor model studies,0.8 ×105viable 4T1breast cancer cells were injected s.c.into the right axilla of BALB/c mice.5days after inoculation,the mice were randomized into four groups:(1)saline(C),(2)paclitaxel-only(P),(3)exercise-only in moderate-intensity(E)(4)exercise in moderate-intensity+paclitaxel(EP),16animals per group.The mice of P and EP group received i.p.injections of paclitaxel(20 mg/kg/w)during the whole experimental period.The mice of other groups received same volume of saline.Exercise groups performed progressive treadmill running up to 17m/min at 0% grade for 30minutes,5d/wk for 5weeks.Results:1)The weight of tumor,tumor volume and ratio of tumor weight and body weight of mice were significantly lower in EP group than C group(P0.05).2)The survival time of mice was prolonged in the group of P,EP and E.The time was significantly longer in EP than other groups(P 0.01).3)The rate of early and late apoptosis cells of mice were significantly higher in EP(P 0.05).4)The expression of NF-κB p65was lower than C group(P0.01),the expression of p-IκBαof mice was lower in EP(P0.01)than C and P group.Conclusion:Moderate intensity exercise combined with paclitaxel can effectively inhibit the growth of tumor,prolong the survival time with tumors in vivo for mice.One of mechanisms of exercise combined with paclitaxel may have a role in development of tumor is that induce apoptosis of tumor cells.