山东体育学院学报
山東體育學院學報
산동체육학원학보
Journal of Shandong Institute Of Physical Education And Sports
2013年
6期
53~56
,共null页
RBP4 胰岛素抵抗 IL-2 IL-2R CD4 CD8 免疫
RBP4 胰島素牴抗 IL-2 IL-2R CD4 CD8 免疫
RBP4 이도소저항 IL-2 IL-2R CD4 CD8 면역
RBP4, insulin resistance, IL - 2, IL - 2R, CD4, CD8, immunity
目的：研究运动对RBP4诱导的胰岛素抵抗大鼠外周血中IL-2、IL-2R的表达以及CD4、CD8活性的影响.方法：8周龄雄性SD大鼠给予重组RBP4 3 μg/g体重腹腔注射,12小时注射一次,持续注射4周.设RBP4＋运动组（RE）、RBP4安静组（RR）和正常安静对照组（C）,RE组予4周无负重游泳训练,60 min/天.免疫组化和免疫印迹法分别检测血清RBP4,胰岛素抵抗指数（HOMA-IR）.ELISA检测血清IL-2、IL-2R的表达;S-P一步法检测血液中CD4、CD8的活性.结果：RE组血清RBP4和HOMA-IR显著低于RR组.RE组较RR组大鼠CD4细胞数增多（P＜0.05）,CD8细胞数稍有减少（P＜0.05）,CD4/CD8比值明显增加（P＜0.01）;血清中IL-2水平明显升高（P＜0.05）,IL-2R水平降低（P＜0.05）,因而比值明显增加（P＜0.01）;RE组与RR组比胸腺指数（P＜0.05）、脾脏指数明显提高（P＜0.01）.结论：RBP4诱导的胰岛素抵抗大鼠免疫功能有所下降.而运动能够明显提升RBP4诱导的胰岛素抵抗大鼠免疫能力.
目的：研究運動對RBP4誘導的胰島素牴抗大鼠外週血中IL-2、IL-2R的錶達以及CD4、CD8活性的影響.方法：8週齡雄性SD大鼠給予重組RBP4 3 μg/g體重腹腔註射,12小時註射一次,持續註射4週.設RBP4＋運動組（RE）、RBP4安靜組（RR）和正常安靜對照組（C）,RE組予4週無負重遊泳訓練,60 min/天.免疫組化和免疫印跡法分彆檢測血清RBP4,胰島素牴抗指數（HOMA-IR）.ELISA檢測血清IL-2、IL-2R的錶達;S-P一步法檢測血液中CD4、CD8的活性.結果：RE組血清RBP4和HOMA-IR顯著低于RR組.RE組較RR組大鼠CD4細胞數增多（P＜0.05）,CD8細胞數稍有減少（P＜0.05）,CD4/CD8比值明顯增加（P＜0.01）;血清中IL-2水平明顯升高（P＜0.05）,IL-2R水平降低（P＜0.05）,因而比值明顯增加（P＜0.01）;RE組與RR組比胸腺指數（P＜0.05）、脾髒指數明顯提高（P＜0.01）.結論：RBP4誘導的胰島素牴抗大鼠免疫功能有所下降.而運動能夠明顯提升RBP4誘導的胰島素牴抗大鼠免疫能力.
목적：연구운동대RBP4유도적이도소저항대서외주혈중IL-2、IL-2R적표체이급CD4、CD8활성적영향.방법：8주령웅성SD대서급여중조RBP4 3 μg/g체중복강주사,12소시주사일차,지속주사4주.설RBP4＋운동조（RE）、RBP4안정조（RR）화정상안정대조조（C）,RE조여4주무부중유영훈련,60 min/천.면역조화화면역인적법분별검측혈청RBP4,이도소저항지수（HOMA-IR）.ELISA검측혈청IL-2、IL-2R적표체;S-P일보법검측혈액중CD4、CD8적활성.결과：RE조혈청RBP4화HOMA-IR현저저우RR조.RE조교RR조대서CD4세포수증다（P＜0.05）,CD8세포수초유감소（P＜0.05）,CD4/CD8비치명현증가（P＜0.01）;혈청중IL-2수평명현승고（P＜0.05）,IL-2R수평강저（P＜0.05）,인이비치명현증가（P＜0.01）;RE조여RR조비흉선지수（P＜0.05）、비장지수명현제고（P＜0.01）.결론：RBP4유도적이도소저항대서면역공능유소하강.이운동능구명현제승RBP4유도적이도소저항대서면역능력.
Objective： To study the effects of exercise on IL -2, IL -2R expression and CD4, CD8 activity in blood of RBP4 - induced insulin resistance rats. Methods：8 - week - old SD male rats were established by intraperitoneal injection with recombination RBP4 3 txg/g,2 times per day for 4 weeks. Model rats were divided into RBP4 ＋ exercise group （ RE ） , RBP4 ＋ rest group （ RR）, and normal control group （C）. RE group rata swam without load,60 rain per day for four weeks. IHC and western blotting were used to detect serum RBP4 and HOMA - IR. ELISA was used to detect serum IL-2,IL-2R expression. S -P method was used to detect activity of CIM and CD8 in the blood. Results：Serum RBP4 and HOMA -IR in RE group were significantly lower than RR group. Comparing to RR group, CIM increased（P 〈0.05） while CD8 decreased（P 〈0.05） in RE group, and CD4/CD8 ratio increased obviously（P 〈0.01 ） in RR group. IL- 2 expression grew obviously（P 〈 0. 05） while IL-2R reduced（P 〈0.05）, so the IL-2/IL-2R ratio increased obviously（P 〈0.01）.Thymus index（P 〈 0.05 ） and spleen index（ P 〈 0.01 ）in RE group rise distinctly when compare with RR group. Conclusion： Immune function of RBP4 - induced insulin resistance rats had been weakend. But exercise can strengthen the immune function of them.
