CAJ | 학술논문

目的：探究早期游泳运动对哺乳期过度喂养肥胖大鼠（OF大鼠）内脏脂肪积累和食欲相关蛋白表达的长期影响，为运动防治哺乳期过度喂养型肥胖提供理论依据。方法：80只SD大鼠妊娠后第3天，随机选取22只母鼠孕期低强度游泳运动至分娩结束（10min／day，6days／week），其余安静至分娩。全部母鼠分娩后第2天随机调整每窝子鼠喂养数目为4只或10只，断乳后按体重配比随机抽取8只OF大鼠进行7周递增游泳运动，其余大鼠7周安静至取材。最终获得实验分组为，孕期安静＋断乳后7周安静正常喂养对照组（CNC2，n=8）、孕期安静＋断乳后7周安静OF组（CSC2，n=8）、孕期游泳运动＋断乳后7周安静OF组（KSC2，n=8）和孕期游泳运动＋断乳后7周游泳运动OF组（ESEl，n=8）。禁食后次日取材，采用免疫组化检测下丘脑弓状核NPY和POMC蛋白表达，放射免疫法测定血清in—sulin、leptin；酶化学法检测血糖和血脂；流式细胞术检测腹后壁脂肪细胞凋亡指数和脂肪细胞数目。结果：1）体成分和摄食量：整个幼龄期ESC2组体重、内脏脂肪垫重和摄食量与CSC2组相比差异不具显著性（P〉0．05）；ESE1组的体重、内脏脂肪垫重均显著低于CSC2组（P〈0．05）。2）血清指标：KSC2组的血清学指标与CSC2组相比差异均不具显著性（P〉0．05）；ESE1组FINS、IGR、leptin和TG指标均显著低于CSC2组OF大鼠（P〈0．05）。3）食欲肽：磷、E1组下丘脑弓状核NPY和POMC蛋白表达量显著高于（X2组（P〈0．01）；KSC2组和CSC2组NPY和POMC蛋白表达量相比差异不具显著性（P〉0．05）。4）脂肪细胞指标：与CSC2组相比，ESEl组腹后壁脂肪细胞凋亡指数显著升高（P〈0．01），脂肪细胞数目则显著降低（P〈0．01）；ESC2组腹后壁脂肪细胞凋亡指数和细胞数目与瞅：2组相比差异均无显著性（P〉0．05）。结论：单纯孕期低强度游泳运动对哺乳期过度喂养型肥胖大鼠没有长期改善效果，孕期结合断乳后7周的早期游泳运动具有改善哺乳期过度喂养型大鼠肥胖的效果。早期游泳运动是否能引发中枢肽一循环激素一脂肪细胞三者协同变化是有效改善哺乳期大鼠过度喂养型肥胖的关键。目的：探究早期遊泳運動對哺乳期過度餵養肥胖大鼠（OF大鼠）內髒脂肪積纍和食欲相關蛋白錶達的長期影響，為運動防治哺乳期過度餵養型肥胖提供理論依據。方法：80隻SD大鼠妊娠後第3天，隨機選取22隻母鼠孕期低彊度遊泳運動至分娩結束（10min／day，6days／week），其餘安靜至分娩。全部母鼠分娩後第2天隨機調整每窩子鼠餵養數目為4隻或10隻，斷乳後按體重配比隨機抽取8隻OF大鼠進行7週遞增遊泳運動，其餘大鼠7週安靜至取材。最終穫得實驗分組為，孕期安靜＋斷乳後7週安靜正常餵養對照組（CNC2，n=8）、孕期安靜＋斷乳後7週安靜OF組（CSC2，n=8）、孕期遊泳運動＋斷乳後7週安靜OF組（KSC2，n=8）和孕期遊泳運動＋斷乳後7週遊泳運動OF組（ESEl，n=8）。禁食後次日取材，採用免疫組化檢測下丘腦弓狀覈NPY和POMC蛋白錶達，放射免疫法測定血清in—sulin、leptin；酶化學法檢測血糖和血脂；流式細胞術檢測腹後壁脂肪細胞凋亡指數和脂肪細胞數目。結果：1）體成分和攝食量：整箇幼齡期ESC2組體重、內髒脂肪墊重和攝食量與CSC2組相比差異不具顯著性（P〉0．05）；ESE1組的體重、內髒脂肪墊重均顯著低于CSC2組（P〈0．05）。2）血清指標：KSC2組的血清學指標與CSC2組相比差異均不具顯著性（P〉0．05）；ESE1組FINS、IGR、leptin和TG指標均顯著低于CSC2組OF大鼠（P〈0．05）。3）食欲肽：燐、E1組下丘腦弓狀覈NPY和POMC蛋白錶達量顯著高于（X2組（P〈0．01）；KSC2組和CSC2組NPY和POMC蛋白錶達量相比差異不具顯著性（P〉0．05）。4）脂肪細胞指標：與CSC2組相比，ESEl組腹後壁脂肪細胞凋亡指數顯著升高（P〈0．01），脂肪細胞數目則顯著降低（P〈0．01）；ESC2組腹後壁脂肪細胞凋亡指數和細胞數目與瞅：2組相比差異均無顯著性（P〉0．05）。結論：單純孕期低彊度遊泳運動對哺乳期過度餵養型肥胖大鼠沒有長期改善效果，孕期結閤斷乳後7週的早期遊泳運動具有改善哺乳期過度餵養型大鼠肥胖的效果。早期遊泳運動是否能引髮中樞肽一循環激素一脂肪細胞三者協同變化是有效改善哺乳期大鼠過度餵養型肥胖的關鍵。
목적：탐구조기유영운동대포유기과도위양비반대서（OF대서）내장지방적루화식욕상관단백표체적장기영향，위운동방치포유기과도위양형비반제공이론의거。방법：80지SD대서임신후제3천，수궤선취22지모서잉기저강도유영운동지분면결속（10min／day，6days／week），기여안정지분면。전부모서분면후제2천수궤조정매와자서위양수목위4지혹10지，단유후안체중배비수궤추취8지OF대서진행7주체증유영운동，기여대서7주안정지취재。최종획득실험분조위，잉기안정＋단유후7주안정정상위양대조조（CNC2，n=8）、잉기안정＋단유후7주안정OF조（CSC2，n=8）、잉기유영운동＋단유후7주안정OF조（KSC2，n=8）화잉기유영운동＋단유후7주유영운동OF조（ESEl，n=8）。금식후차일취재，채용면역조화검측하구뇌궁상핵NPY화POMC단백표체，방사면역법측정혈청in—sulin、leptin；매화학법검측혈당화혈지；류식세포술검측복후벽지방세포조망지수화지방세포수목。결과：1）체성분화섭식량：정개유령기ESC2조체중、내장지방점중화섭식량여CSC2조상비차이불구현저성（P〉0．05）；ESE1조적체중、내장지방점중균현저저우CSC2조（P〈0．05）。2）혈청지표：KSC2조적혈청학지표여CSC2조상비차이균불구현저성（P〉0．05）；ESE1조FINS、IGR、leptin화TG지표균현저저우CSC2조OF대서（P〈0．05）。3）식욕태：린、E1조하구뇌궁상핵NPY화POMC단백표체량현저고우（X2조（P〈0．01）；KSC2조화CSC2조NPY화POMC단백표체량상비차이불구현저성（P〉0．05）。4）지방세포지표：여CSC2조상비，ESEl조복후벽지방세포조망지수현저승고（P〈0．01），지방세포수목칙현저강저（P〈0．01）；ESC2조복후벽지방세포조망지수화세포수목여추：2조상비차이균무현저성（P〉0．05）。결론：단순잉기저강도유영운동대포유기과도위양형비반대서몰유장기개선효과，잉기결합단유후7주적조기유영운동구유개선포유기과도위양형대서비반적효과。조기유영운동시부능인발중추태일순배격소일지방세포삼자협동변화시유효개선포유기대서과도위양형비반적관건。
Objective： To investigate whether early swimming exercise has long-term effects on the appetite peptides of hypothalamus and visceral fat accumulation in overfed rats. Methods： Total 80 SD pregnant rats were enrolled, among which 22 rats began swimming（ 10rain per day, 6days per week）from the 3rd day of pregnancy to delivery . The rest rats kept sedentary till delivery. The pups were distributed randomly and assigned to small litters （4 pups） and normal litters （10 pups） on the second day after birth. 8 overfed pups were randomly selected and assigned to swim 7-week graded swimming training after weanling, the rest kept sedenta- ry. Then we had 4 groups：the pregnancy sedentary＋ normal little control group （CNC2, n= 8）, the pregnancy sedentary＋ OF control group （ CSC2, n = 8）, the pregnancy swimming ＋ OF group （ESC2, n= 8）and pregnancy swimming ＋ post weanling 7wks swimming OF group （ESE1, n= 8）. At the 10 weeks of age the animals were starved overnight and were decapita ted in the next day. NPY and POMC in the arcuate nucleus of hypothalamus were analyzedwith immunohistochemistrical technique. The fasting serum insulin and leptin were assayed by radioimmunoassay, the fasting serum glucose and lipids were assayed by enzymatic methods u sing commercial kits. The apoptotic index and the number of adipocyte in retroperitoneal （RP） fat pad were detected by flow cytometric analysis. Results ： 1 ） Body composition and food con-sumption.. No significant （P〈0. 05） differences were found between the Group ESC2 and CSC2 （P〈0.05） with regard to the body weight,internal fat mass and the food consumption per day at the 10 weeks of age. The body weight, internal fat mass and the food consumption per day were significantly lower in Group ESE1 than that was in Group CSC2（P〈0.01）. 2） Serum parameters No significant （P〈0.05） differences were found between Group ESC2 and Group CSC2 with regard to the Serum parameters at the 10 weeks of age. The serum levels of FIN, IGR, leptin and TG were significantly lower in Group ESE1 than those in Group CSC2 （P〈0.01）. 3）Appetite-Related peptides The positive expressions of NPY and POMC in the arcuate nucleus of hypothalamus were obviously higher in Group ESE1 than those in Group CSC2 （P〈0. 01）. The positive expressions of NPY and POMC were not significant differences in Group ESC2 than those in Group CSC2 （P〈0. 01）. 4）Adipocyte parameters.. RP fat pad apoptotic index was obviously higher in Group ESE1 than that in Group CSC2 （P〈0.01）. While the number of RP adipocyte was obviously lower in Group ESE1 than that in Group CSC2 （P〈0.01）. Conclusion：Maternal swimming itself during pregnancy in order to increas ing the level of physical activity has no long-term prevention effects on the interval fat accumu lation and the body weight programming in the overfed rats;The early swimming during the pregnancy and juvenile could improve the overfed obesity. The synergistic regulation among the central peptides, circulation hormones and the adipocyte is the key for the early swimming lasting improving the overfed obesity.