CAJ | 학술논문

目的：探讨终身跑台、爬梯训练对C57BL／6自然衰老小鼠Sarcopenia关联的自噬相关基因的影响，从而推测自噬在Sarcopenia发生发展中的作用，以及推测运动预防Sarcopenia的作用机理。方法：36只C57BL／6小鼠随机分为青年对照组（YC）、老年对照组（OC）、终身跑台组（LE）、终身爬梯组（LR），每组各9只，其中YC组为3月龄，其余各组自然生长至20月龄。LE组和LR组分别进行为期17个月的跑台和爬梯训练，利用实时荧光定量PCR的生物学方法检测自噬相关基因LC3、Beclinl、和LAMP-2mRNA的表达。结果：1）C57BL／6自然衰老小鼠骨骼肌质量下降，出现严重Sarcopenia怔状。2）与YC组比较，OC组小鼠LC3b、LAMP-2bmRNA表达量显著升高，LC3a、Beclinl和LAMP-2amRNA的变化无显著性差异。3）与OC组相比，终身耐力训练可使LC3a显著升高；使LC3b、Beclinl、LAMP一2a、LAMP一2bmRNA表达量显著降低。4）与OC组相比，终身抗阻训练可使LC3a、Beclinl、LAMP一2amRNA表达量降低，但无显著性差异；使LC3BmRNA得表达量显著降低。5）与LE组相比，LR组Beclinl、LAMP-2a、LAMP-2b表达量升高，日J具有极显著性差异。结论：1）20月龄C57BL／6小鼠符合Sarcopenia研究标准，可作为研究Sarcopenia的模式动物。2）20月龄C57BL／6小鼠腓肠肌巨自噬及CMA活性显著升高，可能造成细胞过度白噬造成蛋白质降解增加，进而诱导产生Sarcopenia。3）终身耐力运动可以有效抑制细胞过度自体吞噬，减少蛋白质降解，缓解Sarcopenia怔状。4）终身抗阻训练可在一定范围内降低自体吞噬活性，增加蛋白质合成，缓解Sarcopenia怔状。5）终身耐力训练控制骨骼肌过度自噬效果要优于终身抗阻训练。
목적：탐토종신포태、파제훈련대C57BL／6자연쇠로소서Sarcopenia관련적자서상관기인적영향，종이추측자서재Sarcopenia발생발전중적작용，이급추측운동예방Sarcopenia적작용궤리。방법：36지C57BL／6소서수궤분위청년대조조（YC）、노년대조조（OC）、종신포태조（LE）、종신파제조（LR），매조각9지，기중YC조위3월령，기여각조자연생장지20월령。LE조화LR조분별진행위기17개월적포태화파제훈련，이용실시형광정량PCR적생물학방법검측자서상관기인LC3、Beclinl、화LAMP-2mRNA적표체。결과：1）C57BL／6자연쇠로소서골격기질량하강，출현엄중Sarcopenia정상。2）여YC조비교，OC조소서LC3b、LAMP-2bmRNA표체량현저승고，LC3a、Beclinl화LAMP-2amRNA적변화무현저성차이。3）여OC조상비，종신내력훈련가사LC3a현저승고；사LC3b、Beclinl、LAMP일2a、LAMP일2bmRNA표체량현저강저。4）여OC조상비，종신항조훈련가사LC3a、Beclinl、LAMP일2amRNA표체량강저，단무현저성차이；사LC3BmRNA득표체량현저강저。5）여LE조상비，LR조Beclinl、LAMP-2a、LAMP-2b표체량승고，일J구유겁현저성차이。결론：1）20월령C57BL／6소서부합Sarcopenia연구표준，가작위연구Sarcopenia적모식동물。2）20월령C57BL／6소서비장기거자서급CMA활성현저승고，가능조성세포과도백서조성단백질강해증가，진이유도산생Sarcopenia。3）종신내력운동가이유효억제세포과도자체탄서，감소단백질강해，완해Sarcopenia정상。4）종신항조훈련가재일정범위내강저자체탄서활성，증가단백질합성，완해Sarcopenia정상。5）종신내력훈련공제골격기과도자서효과요우우종신항조훈련。
Objective： This article is designed to determine the alterations of Autophagy in 20 -nlonths C57BL/6 skeletal muscle with Sareopenia, to observe the effect of a life-long endurance and resistance exercise on the Auto- phagy related to genes expression, and to provide some effective ways that can counterwork Sarcopenia for skeletal muscle. Methods： 36 C57BL/6 mice were randomly divided into young control group （YC） , older control group （OC） , life-long treadmill group （LE） , life-long ladder group （LR） , 9 mice for each group. The age of YC group mice were 3 months and other groups were raised naturally to 20 months. The LE and LR groups were engaged in a treadmill and ladder-climbing training for 17 months respectively. They did exercise once a day and three days a week. Then Real-Time PCR was used to detect the Autophagy related genes LC3, Beclinl a,ld LAMP -2 mRNA expression. Results ： 1 ） With the age growing, skeletal muscle mass decreased significantly in natural aging mice, and the Sarcopenia was occurred in these mice. 2） Compared with the YC group, LC3b, LAMP -2b mRNA ex- pressions were significantly increased in OC group, and LAMP - 2a mRNA expression wash ＇ t significautly changed. 3） Compared with the OC group, lifelong endurance training significantly increased the ratio of LC3a/ LC3b, and decreased Beclinl, LAMP -2a, LAMP -2b mRNA expression. 4） Compared with the OC group, life- long resistance training decreased LC3a, Beclinl, LAMP- 2a mRNA expression, but not significantly; and LC3b mRNA expression decreased significantly. 5） Compared with the LE group, the expressions of Beclinl, LAMP- 2a, LAMP -2b increased a lot. Conclusion： 1 ） 20 months old C57BL/6 mice can be used as the model animal of Sarcopenia. 2） With the age growing, the autophagy flux increased, protein degradation increased, then lead to Sarcopenia. 3 ） Lifelong endurance training can effectively inhibit excessive autophagy, thus ease Sarcopenia symp- toms. 4） Lifelong resistance training can reduce Autophagic activity and protein degradation in a certain range to prevent the development of sarcopenia. 5 ） Life-long endurance training had a better effect on the control of exces- sive autophagy than the life-long resistance training.