北京体育大学学报
北京體育大學學報
북경체육대학학보
Journal of Beijing University of Physical Education
2014年
1期
64~70
,共null页
磷脂酰肌醇-3激酶(PI3K) 蛋白激酶B(PKB Akt) 哺乳动物雷帕霉素靶蛋白(mTOR) 减量训
燐脂酰肌醇-3激酶(PI3K) 蛋白激酶B(PKB Akt) 哺乳動物雷帕黴素靶蛋白(mTOR) 減量訓
린지선기순-3격매(PI3K) 단백격매B(PKB Akt) 포유동물뢰파매소파단백(mTOR) 감량훈
PI3K; PKB/Akt; mTOR; tapering; cardiac muscle ; upstream protein of signal pathway
减量训练对于防止运动性疾病和过度疲劳的发生起重要作用,对于促进心脏朝着有利于运动员机能提高的方向发展至关重要。研究利用72只SPF级7周龄雄性SD大鼠,经过1周适应性训练后,随机分为8组:3周大运动量训练组、3d,6d和2周减量训练组及其相应的对照组。3周大运动量训练采用中等强度和大强度交替进行,运动时间逐渐延长,然后进行减量训练。训练后取左心室肌组织,测定PI3K、Akt的蛋白和磷酸化采用Western blotting方法。结果显示:减量训练过程中,心肌PI3K P85蛋白表达有升高趋势。PI3K是减量训练诱导心肌保护的重要调节物。不同的运动负荷对Akt蛋白表达量和磷酸化产生不同的影响。在3周大运动量训练后进行减量训练,心肌Akt蛋白表达显著增加。在3周大运动量训练后进行6d减量训练,Akt^Ser473磷酸化显著增加,2周减量训练后,心肌Akt^Ser473磷酸化显著增加。减量训练过程中,心肌Akt蛋白表达和Akt^Ser473磷酸化有升高趋势。减量训练通过增加Akt蛋白表达和Akt^Ser473磷酸化对心肌起保护作用。
減量訓練對于防止運動性疾病和過度疲勞的髮生起重要作用,對于促進心髒朝著有利于運動員機能提高的方嚮髮展至關重要。研究利用72隻SPF級7週齡雄性SD大鼠,經過1週適應性訓練後,隨機分為8組:3週大運動量訓練組、3d,6d和2週減量訓練組及其相應的對照組。3週大運動量訓練採用中等彊度和大彊度交替進行,運動時間逐漸延長,然後進行減量訓練。訓練後取左心室肌組織,測定PI3K、Akt的蛋白和燐痠化採用Western blotting方法。結果顯示:減量訓練過程中,心肌PI3K P85蛋白錶達有升高趨勢。PI3K是減量訓練誘導心肌保護的重要調節物。不同的運動負荷對Akt蛋白錶達量和燐痠化產生不同的影響。在3週大運動量訓練後進行減量訓練,心肌Akt蛋白錶達顯著增加。在3週大運動量訓練後進行6d減量訓練,Akt^Ser473燐痠化顯著增加,2週減量訓練後,心肌Akt^Ser473燐痠化顯著增加。減量訓練過程中,心肌Akt蛋白錶達和Akt^Ser473燐痠化有升高趨勢。減量訓練通過增加Akt蛋白錶達和Akt^Ser473燐痠化對心肌起保護作用。
감량훈련대우방지운동성질병화과도피로적발생기중요작용,대우촉진심장조착유리우운동원궤능제고적방향발전지관중요。연구이용72지SPF급7주령웅성SD대서,경과1주괄응성훈련후,수궤분위8조:3주대운동량훈련조、3d,6d화2주감량훈련조급기상응적대조조。3주대운동량훈련채용중등강도화대강도교체진행,운동시간축점연장,연후진행감량훈련。훈련후취좌심실기조직,측정PI3K、Akt적단백화린산화채용Western blotting방법。결과현시:감량훈련과정중,심기PI3K P85단백표체유승고추세。PI3K시감량훈련유도심기보호적중요조절물。불동적운동부하대Akt단백표체량화린산화산생불동적영향。재3주대운동량훈련후진행감량훈련,심기Akt단백표체현저증가。재3주대운동량훈련후진행6d감량훈련,Akt^Ser473린산화현저증가,2주감량훈련후,심기Akt^Ser473린산화현저증가。감량훈련과정중,심기Akt단백표체화Akt^Ser473린산화유승고추세。감량훈련통과증가Akt단백표체화Akt^Ser473린산화대심기기보호작용。
Tapering is important to prevent exercise diseases and overtraining, also it's benefit for heart to improve exercise performance. Seventy-two 7 weeks old male SD rats were in this study. After one week adaptability training they were divided into 8 groups, including 3 weeks high volume training group, 3 days tapering group, 6 clays tapering group, 2 weeks tapering group, and all the paralleled control groups. The rats in 3 weeks high volume training group took moderate intensity exercise and high intensity training alternatively, the training time were gradually increased, then rats took tapering. Tapering was including 3 days, 6 clays and 2 weeks respectively for different groups. After training, the left ventricle cardiac muscle was collected, and the protein expressions and protein phosphorylation of PI3K and Akt were detected by Western blotting. The results showed that PI3K P85 protein expression in cardiac muscle had increased during tapering. It indicated that PI3K was an important modulation for cardiac muscle. Different loads training had different effects on Akt protein expression and Akt phosphorylation. The expression of protein Akt in cardiac muscle increased significantly after 3 weeks high volume exercise combined tapering. The phosphorylation of protein Akt^Ser473 increased significantly after 3 weeks high volume training combined 6 days tapering, as well as after 2 weeks tapering. The expression of protein Akt and phosphorylation of protein Akt^Ser473 in cardiac muscle had increasing trends during tapering. Results demonstrated that tapering could protect cardiac muscle by increasing Akt protein expression and Akt^ Ser473 phosphorylation.