山东体育学院学报
山東體育學院學報
산동체육학원학보
Journal of Shandong Institute Of Physical Education And Sports
2014年
1期
85~89
,共null页
诱导型一氧化氮合酶 单核苷酸多态性 低氧训练
誘導型一氧化氮閤酶 單覈苷痠多態性 低氧訓練
유도형일양화담합매 단핵감산다태성 저양훈련
inducible nitric oxide synthase, single nucleotide polymorphism, hypoxia training
目的：选取低氧反应基因——诱导型一氧化氮合酶（inducible nitric oxide synthase,iN-OS）基因序列上rs10459953、rs1060822单核苷酸多态性（single nucleotide polymorphism,SNP）位点,研究其与低氧训练后生理表型指标关联性.方法：采用关联研究方法（Association-Study）,聚合酶链反应-限制片段长度多态性（PCR-RFLP）实验解析技术,选取35名健康受试者在模拟海拔2500 m高度进行4周高住-高练-低训（living high-exercise high-training low,Hi-Hi-Lo）.研究iNOS基因SNP rs10459953和SNPrs1060822与低氧训练后最大摄氧量（maximal oxygen uptake,VO2 max）、血红蛋白（hemoglobin,Hb）、红细胞数（red blood cell,RBC）等生理表型指标关联性.结果：1）4周低氧训练后,SNP rs10459953不同基因型之间VO2 max、Hb、RBC变化量均无显著性差异.2）4周低氧训练后,SNP rs1060822不同基因型间Hb、RBC变化量有统计学意义（P＜0.05）,其中CT基因型受试者Hb、RBC显著增加（P＜0.05）,且在低氧环境下定量负荷实验中,SNP rs1060822的CT基因型受试者低氧训练后期SpO2动态曲线显著高于初期（P＜0.05）.结论：iNOS基因SNP rs1060822与低氧训练后血象指标存在一定关联性,CT基因型表现出一定优势.
目的：選取低氧反應基因——誘導型一氧化氮閤酶（inducible nitric oxide synthase,iN-OS）基因序列上rs10459953、rs1060822單覈苷痠多態性（single nucleotide polymorphism,SNP）位點,研究其與低氧訓練後生理錶型指標關聯性.方法：採用關聯研究方法（Association-Study）,聚閤酶鏈反應-限製片段長度多態性（PCR-RFLP）實驗解析技術,選取35名健康受試者在模擬海拔2500 m高度進行4週高住-高練-低訓（living high-exercise high-training low,Hi-Hi-Lo）.研究iNOS基因SNP rs10459953和SNPrs1060822與低氧訓練後最大攝氧量（maximal oxygen uptake,VO2 max）、血紅蛋白（hemoglobin,Hb）、紅細胞數（red blood cell,RBC）等生理錶型指標關聯性.結果：1）4週低氧訓練後,SNP rs10459953不同基因型之間VO2 max、Hb、RBC變化量均無顯著性差異.2）4週低氧訓練後,SNP rs1060822不同基因型間Hb、RBC變化量有統計學意義（P＜0.05）,其中CT基因型受試者Hb、RBC顯著增加（P＜0.05）,且在低氧環境下定量負荷實驗中,SNP rs1060822的CT基因型受試者低氧訓練後期SpO2動態麯線顯著高于初期（P＜0.05）.結論：iNOS基因SNP rs1060822與低氧訓練後血象指標存在一定關聯性,CT基因型錶現齣一定優勢.
목적：선취저양반응기인——유도형일양화담합매（inducible nitric oxide synthase,iN-OS）기인서렬상rs10459953、rs1060822단핵감산다태성（single nucleotide polymorphism,SNP）위점,연구기여저양훈련후생리표형지표관련성.방법：채용관련연구방법（Association-Study）,취합매련반응-한제편단장도다태성（PCR-RFLP）실험해석기술,선취35명건강수시자재모의해발2500 m고도진행4주고주-고련-저훈（living high-exercise high-training low,Hi-Hi-Lo）.연구iNOS기인SNP rs10459953화SNPrs1060822여저양훈련후최대섭양량（maximal oxygen uptake,VO2 max）、혈홍단백（hemoglobin,Hb）、홍세포수（red blood cell,RBC）등생리표형지표관련성.결과：1）4주저양훈련후,SNP rs10459953불동기인형지간VO2 max、Hb、RBC변화량균무현저성차이.2）4주저양훈련후,SNP rs1060822불동기인형간Hb、RBC변화량유통계학의의（P＜0.05）,기중CT기인형수시자Hb、RBC현저증가（P＜0.05）,차재저양배경하정량부하실험중,SNP rs1060822적CT기인형수시자저양훈련후기SpO2동태곡선현저고우초기（P＜0.05）.결론：iNOS기인SNP rs1060822여저양훈련후혈상지표존재일정관련성,CT기인형표현출일정우세.
Objective： In this study, we selected two single nucleotide polymorphisms （SNP）, rs10459953 and rs1060822, in the hypoxia response gene, inducible nitric oxide synthase（iNOS） , to investigate association between these polymorphisms of iNOS gene and physiological phenotype parameters response to hypoxia training. Methods ：35 healthy subjects completed a g weeks＂living high - exercise high - training low＂ in a simulated ahitude of 2500m height, and using association - study of polymerase chain reaction- restriction fragment length polymorphism（ PCR- RFLP） for genotype analysis. To investigate association between SNP rs10459953 and rs1060822 of iNOS gene and physi- ological phenotype parameters including maximal oxygen uptake （VO2max）, hemoglobin （Hb）, red blood cell （ RBC ） and other parameters after hypoxia training. Results ： （ 1 ） After the 4 weeks hypoxia ＇ training,there was no significant difference in VOEmax, Hb or RBC between different genotypes of SNP rs10459953. （2） After the 4 weeks hypoxia training, carriers with CT genotype of SNP rs1060822 showed significantly higher increase in Hb and RBC （ P 〈 0.05 ）. There were significant differences in carriers with CT genetype of SNP rs1060822 in SpO2 during period of exercising before and after 4 weeks hypoxia training （ P 〈 0.05 ） , and SpO2 curve was higher after hypoxia training. Conclusion ：These findings suggested that SNP rM060822 of iNOS gene was possibly associated with hemogram parameters after hypoxia training, and CT genotype showed a certain advantage.
