山东体育科技
山東體育科技
산동체육과기
Shandong Sports Science & Technology
2014年
1期
67~71
,共null页
运动 左旋肉碱 肥胖 肝脏 胆固醇
運動 左鏇肉堿 肥胖 肝髒 膽固醇
운동 좌선육감 비반 간장 담고순
exercise ; L - carnitine ; obesity ; liver; cholesterol
目的：探讨运动与肉碱补充对肝脏胆固醇代谢的影响.方法：通过饲喂高脂日粮将C57BL/6小鼠建立营养性肥胖模型.随机分为4组：对照组、运动组、左旋肉碱组及运动结合左旋肉碱组.5周后采集血液、肝脏组织,检测血清和肝脏中的胆固醇水平及胆固醇代谢关键基因3-羟-3-甲基戊二酸单酰CoA还原酶（HMGCR）、肝脏X受体（LXR）和固醇调节元件结合蛋白2（SREBP2）的基因表达.结果：无论是运动还是左旋肉碱补充均使肥胖小鼠体重显著降低,而运动跟肉碱同时处理对体重的影响更大.运动显著增高血清中总胆固醇（Tch）的水平（P＜0.05）,并且显著增高低密度脂蛋白（LDL）的水平（P＜0.05）.运动与肉碱同时处理显著增高血清高密度脂蛋白（HDL）的水平（P＜0.05）.运动及运动与肉碱同时处理均显著上调胆固醇合成的限速酶——HMGCR的蛋白表达（P＜0.05）,并且与单纯肉碱组相比,运动对HMGCR的蛋白表达有上调的趋势（P=0.073）.运动与肉碱同时处理显著上调LXR和SREBP2的基因表达（P＜0.05）.结论：运动结合左旋肉碱补充通过刺激HMGCR、LXR和SREBP2的表达上调HDL的水平,运动主要通过HMGCR调节胆固醇及脂蛋白的代谢,而单纯左旋肉碱补充对胆固醇代谢没有显著影响.
目的：探討運動與肉堿補充對肝髒膽固醇代謝的影響.方法：通過飼餵高脂日糧將C57BL/6小鼠建立營養性肥胖模型.隨機分為4組：對照組、運動組、左鏇肉堿組及運動結閤左鏇肉堿組.5週後採集血液、肝髒組織,檢測血清和肝髒中的膽固醇水平及膽固醇代謝關鍵基因3-羥-3-甲基戊二痠單酰CoA還原酶（HMGCR）、肝髒X受體（LXR）和固醇調節元件結閤蛋白2（SREBP2）的基因錶達.結果：無論是運動還是左鏇肉堿補充均使肥胖小鼠體重顯著降低,而運動跟肉堿同時處理對體重的影響更大.運動顯著增高血清中總膽固醇（Tch）的水平（P＜0.05）,併且顯著增高低密度脂蛋白（LDL）的水平（P＜0.05）.運動與肉堿同時處理顯著增高血清高密度脂蛋白（HDL）的水平（P＜0.05）.運動及運動與肉堿同時處理均顯著上調膽固醇閤成的限速酶——HMGCR的蛋白錶達（P＜0.05）,併且與單純肉堿組相比,運動對HMGCR的蛋白錶達有上調的趨勢（P=0.073）.運動與肉堿同時處理顯著上調LXR和SREBP2的基因錶達（P＜0.05）.結論：運動結閤左鏇肉堿補充通過刺激HMGCR、LXR和SREBP2的錶達上調HDL的水平,運動主要通過HMGCR調節膽固醇及脂蛋白的代謝,而單純左鏇肉堿補充對膽固醇代謝沒有顯著影響.
목적：탐토운동여육감보충대간장담고순대사적영향.방법：통과사위고지일량장C57BL/6소서건립영양성비반모형.수궤분위4조：대조조、운동조、좌선육감조급운동결합좌선육감조.5주후채집혈액、간장조직,검측혈청화간장중적담고순수평급담고순대사관건기인3-간-3-갑기무이산단선CoA환원매（HMGCR）、간장X수체（LXR）화고순조절원건결합단백2（SREBP2）적기인표체.결과：무론시운동환시좌선육감보충균사비반소서체중현저강저,이운동근육감동시처리대체중적영향경대.운동현저증고혈청중총담고순（Tch）적수평（P＜0.05）,병차현저증고저밀도지단백（LDL）적수평（P＜0.05）.운동여육감동시처리현저증고혈청고밀도지단백（HDL）적수평（P＜0.05）.운동급운동여육감동시처리균현저상조담고순합성적한속매——HMGCR적단백표체（P＜0.05）,병차여단순육감조상비,운동대HMGCR적단백표체유상조적추세（P=0.073）.운동여육감동시처리현저상조LXR화SREBP2적기인표체（P＜0.05）.결론：운동결합좌선육감보충통과자격HMGCR、LXR화SREBP2적표체상조HDL적수평,운동주요통과HMGCR조절담고순급지단백적대사,이단순좌선육감보충대담고순대사몰유현저영향.
Objective：To investigate the effects of exercise and L- carnitine supplementation on cholesterol metabolism in the liver. Methods：To establish nutritional obesity model by fedding high- fat diets to C57BL/6 mice. Obesity mice were randomly divided into 4 groups, tile control group, the exercise group, fedding L- carnitine group and exercise combined L- carnitine supplementation group. 5 weeks later, miee were killed, and blood, liver tissue were collected. Lipids in serum and cholesterol metabolism key enzymes 3 - hydroxy - 3 - methyl - glutao＇l CoA reductase （ HMGCR ）, liver X receptor （ LXR ） and sterol regulatory element binding protein 2 （SREBP2） in liver were tested. Results ：The body weights were significantly low in 3 experiment groups （ P 〈 0. 05 ）, especially in exercise combined L - carnitine supplementation group. Total cholesterol （Tch） and low density lipoprotein （LDL） in serum were significantly increased in exercise group （P 〈 O. 05）. High density lipoprotein （HDL） in serum was significantly increased in exercise combined L- carnitine supplementation group（ P 〈 O. 05）. The expression of cholesterol synthesis rate - limiting enzyme - HMGCR was significantly up- regulated in exercise group and in exercise combined L- carnitine supplementation group （P 〈 O. 05 ） , and compared with fedding L - carnitine group, there was upregulated tendency of HMGCR protein expression （P = O. 073 ） in exercise group. The expression of LXR and SREBP2 was upregulated in exercise combined L - carnitine supplementation group （P 〈 O. 05 ）. Conclusion： Exercise combined with L - carnitine supplemental＇. may up - regulated the concentration of HDL by stimulation expression of HMGCR, LXR and SREBP2. Exercise regulates the metabolism of cholesterol and lipoprotein mainly through LXR and SREBP2, while L - carnitine supplementation major influence HMGCR to regulate cholesterol metabolism.
