武汉体育学院学报
武漢體育學院學報
무한체육학원학보
Journal of Wuhan Institute of Physical Education
2014年
3期
76~81
,共null页
骨骼肌 细胞自噬 Beclin1 Atg9 Atg7 LC-3 急性递增负荷运动 时相性
骨骼肌 細胞自噬 Beclin1 Atg9 Atg7 LC-3 急性遞增負荷運動 時相性
골격기 세포자서 Beclin1 Atg9 Atg7 LC-3 급성체증부하운동 시상성
skeletal muscle; autophagy; Beclinl; Atgg; Atg7; LC-3; incremental load exercise; different phases
目的：通过建立急性递增负荷运动的时相性模型,检测骨骼肌细胞自噬启动阶段关键基因Bec-lin1、膜螯合关键基因Atg9、自噬泡延伸阶段关键基因Atg7和微管相关蛋白1轻链3（LC-3）表达的动态变化,探讨自噬发生不同阶段的关键基因在运动中的适应性情况.方法：40只健康雄性ICR小鼠,7～8周龄,体重19～21g,随机分为安静组（Con）、急性递增负荷运动45min（E45）、90min（E90）、120min（E120）和150min（E150）组,各组均8只.小鼠进行适应性跑台运动,坡度0°,速度0.8km/h,持续时间5min,共训练3天.正式运动遵循以下程序：第1级负荷：1km/h,15min;第2级负荷：1.2km/h,15min;第3级负荷：1.4～1.5km/h,各组分别运动到相应时间点.安静组于安静状态、运动组于运动中相应时间点即刻断颈椎处死,迅速取出完整的下肢腓肠肌.实时荧光定量PCR（Real-time PCR）检测骨骼肌Beclin1、Atg9、Atg7、LC-3基因表达水平.结果：（1）骨骼肌Beclin1 mRNA表达在递增负荷运动开始即急剧上升,在45min达到峰值,随后又呈现持续下降态势.（2）骨骼肌Atg9、Atg7 mRNA表达在递增负荷运动开始即稳步上升,在90min达到峰值,并维持一段时间,随后又呈现稳步下降态势.（3）骨骼肌LC-3 mRNA表达与安静组相比以及各组间相比均未见显著性差异.结论：自噬对递增负荷运动具有较好的应答能力,但这并不总是持续不变的,随着运动时间的延长,其应答能力逐渐降低,表现出一种良好的应激-适应的反应机制.
目的：通過建立急性遞增負荷運動的時相性模型,檢測骨骼肌細胞自噬啟動階段關鍵基因Bec-lin1、膜螯閤關鍵基因Atg9、自噬泡延伸階段關鍵基因Atg7和微管相關蛋白1輕鏈3（LC-3）錶達的動態變化,探討自噬髮生不同階段的關鍵基因在運動中的適應性情況.方法：40隻健康雄性ICR小鼠,7～8週齡,體重19～21g,隨機分為安靜組（Con）、急性遞增負荷運動45min（E45）、90min（E90）、120min（E120）和150min（E150）組,各組均8隻.小鼠進行適應性跑檯運動,坡度0°,速度0.8km/h,持續時間5min,共訓練3天.正式運動遵循以下程序：第1級負荷：1km/h,15min;第2級負荷：1.2km/h,15min;第3級負荷：1.4～1.5km/h,各組分彆運動到相應時間點.安靜組于安靜狀態、運動組于運動中相應時間點即刻斷頸椎處死,迅速取齣完整的下肢腓腸肌.實時熒光定量PCR（Real-time PCR）檢測骨骼肌Beclin1、Atg9、Atg7、LC-3基因錶達水平.結果：（1）骨骼肌Beclin1 mRNA錶達在遞增負荷運動開始即急劇上升,在45min達到峰值,隨後又呈現持續下降態勢.（2）骨骼肌Atg9、Atg7 mRNA錶達在遞增負荷運動開始即穩步上升,在90min達到峰值,併維持一段時間,隨後又呈現穩步下降態勢.（3）骨骼肌LC-3 mRNA錶達與安靜組相比以及各組間相比均未見顯著性差異.結論：自噬對遞增負荷運動具有較好的應答能力,但這併不總是持續不變的,隨著運動時間的延長,其應答能力逐漸降低,錶現齣一種良好的應激-適應的反應機製.
목적：통과건립급성체증부하운동적시상성모형,검측골격기세포자서계동계단관건기인Bec-lin1、막오합관건기인Atg9、자서포연신계단관건기인Atg7화미관상관단백1경련3（LC-3）표체적동태변화,탐토자서발생불동계단적관건기인재운동중적괄응성정황.방법：40지건강웅성ICR소서,7～8주령,체중19～21g,수궤분위안정조（Con）、급성체증부하운동45min（E45）、90min（E90）、120min（E120）화150min（E150）조,각조균8지.소서진행괄응성포태운동,파도0°,속도0.8km/h,지속시간5min,공훈련3천.정식운동준순이하정서：제1급부하：1km/h,15min;제2급부하：1.2km/h,15min;제3급부하：1.4～1.5km/h,각조분별운동도상응시간점.안정조우안정상태、운동조우운동중상응시간점즉각단경추처사,신속취출완정적하지비장기.실시형광정량PCR（Real-time PCR）검측골격기Beclin1、Atg9、Atg7、LC-3기인표체수평.결과：（1）골격기Beclin1 mRNA표체재체증부하운동개시즉급극상승,재45min체도봉치,수후우정현지속하강태세.（2）골격기Atg9、Atg7 mRNA표체재체증부하운동개시즉은보상승,재90min체도봉치,병유지일단시간,수후우정현은보하강태세.（3）골격기LC-3 mRNA표체여안정조상비이급각조간상비균미견현저성차이.결론：자서대체증부하운동구유교호적응답능력,단저병불총시지속불변적,수착운동시간적연장,기응답능력축점강저,표현출일충량호적응격-괄응적반응궤제.
This paper aimed to set up different phase models of acute incremental load exercise, and investigated the continuous changes of Beclinl, Atg9, Atg7 and LC-3 mRNA transcription in skeletal muscle with quantitative real--time PCR. The purpose was to know whether or not they could adapt to the incremental load exercise. 40 male ICR mice（7-8 weeks old, weight 19-21g） were randomly divided into control group （Con）, acute incremental load exercise for 45min group （E45）, 90min group （E90）, 120min group （El20） and 150min group （E150）, with 8 mice in each group. Three days adaptive treadmill training was finished before formal treadmill exercise （0% grade, 0. 8km/h, 5min）. Quantitative real-time PCR was used to detect the skeletal muscle Beclinl, Atg9, Atg7 as well as LC-- 3 mRNA gene expressions in each group. It was seen that the skeletal muscle Beclinl mRNA gene expression began to rise in the early stage of acute incremental load exercise and increased significantly at 45 min, and then it began to drop quickly. The skeletal muscle Atg9 and Atg7 mRNA gene expressions began to rise in the early stage of acute in- cremental load exercise and increased significantly at 90 min, after maintaining which for a few minutes, they began to drop quickly. The skeletal muscle LC-3 mRNA gene expression did not change significantly compared with the control group during the different stage of acute incremental load exercise. It could be concluded that autophagy might respond much to the incremental load exercise. However, its response ability gradually decreased with the extension of exercise, showing a mechanism of ＂stress-adaptation＂.
