天津体育学院学报
天津體育學院學報
천진체육학원학보
Journal of Tianjin Institute of Physical Education
2014年
1期
1~5
,共null页
Ⅱ型糖尿病 有氧运动 肾脏损伤 Notch-1信号通路 动物实验
Ⅱ型糖尿病 有氧運動 腎髒損傷 Notch-1信號通路 動物實驗
Ⅱ형당뇨병 유양운동 신장손상 Notch-1신호통로 동물실험
type 2 diabetes; aerobic exercise; renal injury; Notch-1 signaling pathway; animal experiment
目的：观察有氧游泳运动对Ⅱ型糖尿病大鼠肾脏损伤的改善效果,并探讨其可能的机制.方法：45只4周龄健康雄性SD大鼠,随机抽取10只为正常对照组,基础饲料喂养;其余35只经高糖高脂喂养5周后,配合腹腔注射STZ（35 mg/kg.bw）诱导建立Ⅱ型糖尿病大鼠模型;7周后,将成模大鼠随机分为糖尿病安静组和糖尿病运动组,每组14只.3组大鼠均采用基础饲料喂养;糖尿病运动组大鼠进行8周有氧游泳运动;正常对照组、糖尿病安静组2组大鼠均自由活动,不施加任何干预.结果：（1）8周有氧游泳运动后,糖尿病运动组肾脏在电镜下的形态表现为肾小球三层结构较清晰,基底膜增厚不明显,足突融合减少,溶酶体增多现象等均有一定程度减少,较糖尿病安静组有明显改善;（2）糖尿病运动组血糖浓度和24 h UA排泄量较糖尿病安静组显著降低（分别为P＜0.05或P＜0.01）;（3）糖尿病运动组肾皮质Jagged-1、Val1744NICD和Hes-1蛋白的表达较糖尿病安静组均显著降低（P＜0.05或P＜0.01）.结论：运动可提高Ⅱ型糖尿病大鼠肾功能,改善大鼠肾脏损伤,可能与其下调Ⅱ型糖尿病状态下激活的Notch-1信号通路有关.
目的：觀察有氧遊泳運動對Ⅱ型糖尿病大鼠腎髒損傷的改善效果,併探討其可能的機製.方法：45隻4週齡健康雄性SD大鼠,隨機抽取10隻為正常對照組,基礎飼料餵養;其餘35隻經高糖高脂餵養5週後,配閤腹腔註射STZ（35 mg/kg.bw）誘導建立Ⅱ型糖尿病大鼠模型;7週後,將成模大鼠隨機分為糖尿病安靜組和糖尿病運動組,每組14隻.3組大鼠均採用基礎飼料餵養;糖尿病運動組大鼠進行8週有氧遊泳運動;正常對照組、糖尿病安靜組2組大鼠均自由活動,不施加任何榦預.結果：（1）8週有氧遊泳運動後,糖尿病運動組腎髒在電鏡下的形態錶現為腎小毬三層結構較清晰,基底膜增厚不明顯,足突融閤減少,溶酶體增多現象等均有一定程度減少,較糖尿病安靜組有明顯改善;（2）糖尿病運動組血糖濃度和24 h UA排洩量較糖尿病安靜組顯著降低（分彆為P＜0.05或P＜0.01）;（3）糖尿病運動組腎皮質Jagged-1、Val1744NICD和Hes-1蛋白的錶達較糖尿病安靜組均顯著降低（P＜0.05或P＜0.01）.結論：運動可提高Ⅱ型糖尿病大鼠腎功能,改善大鼠腎髒損傷,可能與其下調Ⅱ型糖尿病狀態下激活的Notch-1信號通路有關.
목적：관찰유양유영운동대Ⅱ형당뇨병대서신장손상적개선효과,병탐토기가능적궤제.방법：45지4주령건강웅성SD대서,수궤추취10지위정상대조조,기출사료위양;기여35지경고당고지위양5주후,배합복강주사STZ（35 mg/kg.bw）유도건립Ⅱ형당뇨병대서모형;7주후,장성모대서수궤분위당뇨병안정조화당뇨병운동조,매조14지.3조대서균채용기출사료위양;당뇨병운동조대서진행8주유양유영운동;정상대조조、당뇨병안정조2조대서균자유활동,불시가임하간예.결과：（1）8주유양유영운동후,당뇨병운동조신장재전경하적형태표현위신소구삼층결구교청석,기저막증후불명현,족돌융합감소,용매체증다현상등균유일정정도감소,교당뇨병안정조유명현개선;（2）당뇨병운동조혈당농도화24 h UA배설량교당뇨병안정조현저강저（분별위P＜0.05혹P＜0.01）;（3）당뇨병운동조신피질Jagged-1、Val1744NICD화Hes-1단백적표체교당뇨병안정조균현저강저（P＜0.05혹P＜0.01）.결론：운동가제고Ⅱ형당뇨병대서신공능,개선대서신장손상,가능여기하조Ⅱ형당뇨병상태하격활적Notch-1신호통로유관.
Objective ： To investigate the effect of aerobic swimming exercise on improving renal injury in type 2 diabetic rats and its mechanism. Methods ： Forty-five male Spragne-Dawley （SD） rats for 4 weeks were randomly selected 10 SD rats as normal control group by normal diet feeding. The model of type 2 diabetic rats was duplicated in the study through 35 SD rats fed high-sugar-fat diet for five weeks together with intraperitoneal infecting of low dose of STZ （35mg/kg.bw）. After 7 weeks the model rats were randomly divided into diabetic control group （N=14） and diabetic exercise group （N=14）. The three groups were all fed by normal diet feeding. Diabetic exercise group was underwent the intervention of swimming exercise for 8 weeks, while normal control group and diabetic control group moved freely without any intervention. Results： （1）After swimming exercise for 8 weeks, the group treated by exercise could be found through electron microscope that it had clearer glomerular structure, such as no-obvious thickened glomerular basement membrane, decreased fusion of foot process, and reduced lysosome of renal tubular cells, and so on, which improved significantly comparing with diabetic control group. （2） Compared with dia- betic control group, the concentrations of blood glucose and the 24h mieroalbuminuria excretion in diabetic exercise group decreased significantly （P〈0.05 or P〈0.01）. （3） Compared with diabetic control group, diabetic exercise group of Jagged-1 and Val1744NICD and Hes-1 expressions in the renal cortex de- creased significantly （P〈0.05 or P〈0.01 ）. Conclusions： Exercise can protect renal function and improve renal injury for type 2 diabetes, which may be relat- ed to downregulating the activity of Notch- 1 signaling stimulated under type 2 diabetes conditions.
